################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccIII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp700I Asp718I AviII AvrII BbeI BbvCI BcnI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI HaeII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmeI PshAI PvuI RcaI RsrII SacI SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccI 898 GTAGAC GT^[AC][GT]AC AclI 2541 AACGTT AA^CGTT AcyI 18 GACGCC G[GA]^CG[CT]C AflII 1437 CTTAAG C^TTAAG AflIII 221 ACATGT A^C[GA][CT]GT AhdI 1888 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1462 CCTGAGG CC^T[ACGT]AGG AseI 238 ATTAAT AT^TAAT AsnI 238 ATTAAT AT^TAAT AspEI 1888 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 640 GTGCTC G[AT]GC[AT]^C AspI 649 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 643 CTCGAG C^[CT]CG[GA]G BamHI 2229 GGATCC G^GATCC BanI 676 GGCACC G^G[CT][GA]CC BanII 1263 GGGCTC G[GA]GC[CT]^C BbrPI 298 CACGTG CAC^GTG BcgI 677 GCACCGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 548 GTATCC GTATCC(6/5) BclI 2414 TGATCA T^GATCA BfrI 1437 CTTAAG C^TTAAG BglII 1179 AGATCT A^GATCT BsaHI 18 GACGCC G[GA]^CG[CT]C BsaI 1020 GAGACC GGTCTC(1/5) BsiHKAI 640 GTGCTC G[AT]GC[AT]^C BsmBI 211 GAGACG CGTCTC(1/5) BsoBI 643 CTCGAG C^[CT]CG[GA]G BspLU11I 221 ACATGT A^CATGT BspMI 1011 GCAGGT ACCTGC(4/8) BsrBI 96 GAGCGG CCGCTC(-3/-3) BsrDI 1295 GCAATG GCAATG(2/0) BstXI 2085 CCACGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1462 CCTGAGG CC^T[ACGT]AGG CfoI 63 GCGC GCG^C Eam1105I 1888 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1462 CCTGAGG CC^T[ACGT]AGG EcoNI 758 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1717 GATATC GAT^ATC EcoT14I 888 CCAAGG C^C[AT][AT]GG FbaI 2414 TGATCA T^GATCA HhaI 63 GCGC GCG^C Hin1I 18 GACGCC G[GA]^CG[CT]C HinP1I 63 GCGC G^CGC HincII 949 GTTGAC GT[CT]^[GA]AC HindII 949 GTTGAC GT[CT]^[GA]AC HindIII 2515 AAGCTT A^AGCTT KspI 552 CCGCGG CCGC^GG PaeR7I 643 CTCGAG C^TCGAG PflFI 649 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 298 CACGTG CAC^GTG PmlI 298 CACGTG CAC^GTG PshBI 238 ATTAAT AT^TAAT Psp1406I 2541 AACGTT AA^CGTT PstI 1778 CTGCAG CTGCA^G SacII 552 CCGCGG CCGC^GG ScaI 742 AGTACT AGT^ACT SexAI 902 ACCAGGT A^CC[AT]GGT SphI 2078 GCATGC GCATG^C SspI 1655 AATATT AAT^ATT StuI 521 AGGCCT AGG^CCT StyI 888 CCAAGG C^C[AT][AT]GG Tth111I 649 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1368 CCAGTCGTCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 643 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 553 CGCG CG^CG 2118 CGCG BalI 480 TGGCCA TGG^CCA 885 TGGCCA BmyI 640 GTGCTC G[AGT]GC[ACT]^C 1263 GGGCTC BsaAI 298 CACGTG [CT]AC^GT[GA] 545 CACGTA BsgI 1095 CTGCAC GTGCAG(16/14) 301 GTGCAG Bsp1286I 640 GTGCTC G[AGT]GC[ACT]^C 1263 GGGCTC BstUI 553 CGCG CG^CG 2118 CGCG DsaI 552 CCGCGG C^C[GA][CT]GG 680 CCGTGG HgaI 18 GACGC GACGC(5/10) 790 GACGC MluNI 480 TGGCCA TGG^CCA 885 TGGCCA MscI 480 TGGCCA TGG^CCA 885 TGGCCA MvnI 553 CGCG CG^CG 2118 CGCG NspI 221 ACATGT [GA]CATG^[CT] 2078 GCATGC PvuII 141 CAGCTG CAG^CTG 2002 CAGCTG SfcI 1046 CTATAG C^T[GA][CT]AG 1778 CTGCAG XbaI 780 TCTAGA T^CTAGA 2610 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BbsI BbvI BfaI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII