##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AviII AvrII BbeI BbvCI
BcnI BglI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FseI
FspI HaeII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmeI
PshAI PvuI RcaI RsrII SacI
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Van91I XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 898 GTAGAC GT^[AC][GT]AC
AclI 2541 AACGTT AA^CGTT
AcyI 18 GACGCC G[GA]^CG[CT]C
AflII 1437 CTTAAG C^TTAAG
AflIII 221 ACATGT A^C[GA][CT]GT
AhdI 1888 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1462 CCTGAGG CC^T[ACGT]AGG
AseI 238 ATTAAT AT^TAAT
AsnI 238 ATTAAT AT^TAAT
AspEI 1888 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 640 GTGCTC G[AT]GC[AT]^C
AspI 649 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 643 CTCGAG C^[CT]CG[GA]G
BamHI 2229 GGATCC G^GATCC
BanI 676 GGCACC G^G[CT][GA]CC
BanII 1263 GGGCTC G[GA]GC[CT]^C
BbrPI 298 CACGTG CAC^GTG
BcgI 677 GCACCGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 548 GTATCC GTATCC(6/5)
BclI 2414 TGATCA T^GATCA
BfrI 1437 CTTAAG C^TTAAG
BglII 1179 AGATCT A^GATCT
BsaHI 18 GACGCC G[GA]^CG[CT]C
BsaI 1020 GAGACC GGTCTC(1/5)
BsiHKAI 640 GTGCTC G[AT]GC[AT]^C
BsmBI 211 GAGACG CGTCTC(1/5)
BsoBI 643 CTCGAG C^[CT]CG[GA]G
BspLU11I 221 ACATGT A^CATGT
BspMI 1011 GCAGGT ACCTGC(4/8)
BsrBI 96 GAGCGG CCGCTC(-3/-3)
BsrDI 1295 GCAATG GCAATG(2/0)
BstXI 2085 CCACGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1462 CCTGAGG CC^T[ACGT]AGG
CfoI 63 GCGC GCG^C
Eam1105I 1888 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1462 CCTGAGG CC^T[ACGT]AGG
EcoNI 758 CCTTTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1717 GATATC GAT^ATC
EcoT14I 888 CCAAGG C^C[AT][AT]GG
FbaI 2414 TGATCA T^GATCA
HhaI 63 GCGC GCG^C
Hin1I 18 GACGCC G[GA]^CG[CT]C
HinP1I 63 GCGC G^CGC
HincII 949 GTTGAC GT[CT]^[GA]AC
HindII 949 GTTGAC GT[CT]^[GA]AC
HindIII 2515 AAGCTT A^AGCTT
KspI 552 CCGCGG CCGC^GG
PaeR7I 643 CTCGAG C^TCGAG
PflFI 649 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 298 CACGTG CAC^GTG
PmlI 298 CACGTG CAC^GTG
PshBI 238 ATTAAT AT^TAAT
Psp1406I 2541 AACGTT AA^CGTT
PstI 1778 CTGCAG CTGCA^G
SacII 552 CCGCGG CCGC^GG
ScaI 742 AGTACT AGT^ACT
SexAI 902 ACCAGGT A^CC[AT]GGT
SphI 2078 GCATGC GCATG^C
SspI 1655 AATATT AAT^ATT
StuI 521 AGGCCT AGG^CCT
StyI 888 CCAAGG C^C[AT][AT]GG
Tth111I 649 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1368 CCAGTCGTCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 643 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccII 553 CGCG CG^CG
2118 CGCG
BalI 480 TGGCCA TGG^CCA
885 TGGCCA
BmyI 640 GTGCTC G[AGT]GC[ACT]^C
1263 GGGCTC
BsaAI 298 CACGTG [CT]AC^GT[GA]
545 CACGTA
BsgI 1095 CTGCAC GTGCAG(16/14)
301 GTGCAG
Bsp1286I 640 GTGCTC G[AGT]GC[ACT]^C
1263 GGGCTC
BstUI 553 CGCG CG^CG
2118 CGCG
DsaI 552 CCGCGG C^C[GA][CT]GG
680 CCGTGG
HgaI 18 GACGC GACGC(5/10)
790 GACGC
MluNI 480 TGGCCA TGG^CCA
885 TGGCCA
MscI 480 TGGCCA TGG^CCA
885 TGGCCA
MvnI 553 CGCG CG^CG
2118 CGCG
NspI 221 ACATGT [GA]CATG^[CT]
2078 GCATGC
PvuII 141 CAGCTG CAG^CTG
2002 CAGCTG
SfcI 1046 CTATAG C^T[GA][CT]AG
1778 CTGCAG
XbaI 780 TCTAGA T^CTAGA
2610 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbsI BbvI
BfaI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII