################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AlwNI ApaI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BpuAI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I EcoRV EcoT22I FauI FbaI FseI FspI KasI KpnI Ksp632I KspI MaeII MfeI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 422 GTCGAC GT^[AC][GT]AC AccII 1621 CGCG CG^CG AcyI 365 GACGCC G[GA]^CG[CT]C Alw44I 1140 GTGCAC G^TGCAC Aor51HI 392 AGCGCT AGC^GCT ApaLI 1140 GTGCAC G^TGCAC BbvCI 482 GCTGAGG CCTCAGC(-5/-2) BsaBI 2046 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 365 GACGCC G[GA]^CG[CT]C BsaI 1487 GGTCTC GGTCTC(1/5) BsgI 997 CTGCAC GTGCAG(16/14) BsmAI 1488 GTCTC GTCTC(1/5) BsrDI 477 GCAATG GCAATG(2/0) BstEII 1691 GGTCACC G^GT[ACGT]ACC BstPI 1691 GGTCACC G^GT[ACGT]ACC BstUI 1621 CGCG CG^CG BstXI 1543 CCACGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 440 CGGCCA [CT]^GGCC[GA] Eco47III 392 AGCGCT AGC^GCT EcoO65I 1691 GGTCACC G^GT[ACGT]ACC EcoRI 685 GAATTC G^AATTC HaeII 392 AGCGCT [GA]GCGC^[CT] Hin1I 365 GACGCC G[GA]^CG[CT]C HindIII 207 AAGCTT A^AGCTT HpaI 1390 GTTAAC GTT^AAC MamI 2046 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1621 CGCG CG^CG NcoI 1959 CCATGG C^CATGG NspI 873 GCATGC [GA]CATG^[CT] SalI 422 GTCGAC G^TCGAC SphI 873 GCATGC GCATG^C SspI 2528 AATATT AAT^ATT SwaI 2417 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AciI 529 GCGG CCGC(-3/-1) 733 GCGG AfaI 272 GTAC GT^AC 1761 GTAC AocI 309 CCTCAGG CC^T[ACGT]AGG 1748 CCTGAGG AseI 2012 ATTAAT AT^TAAT 2460 ATTAAT AsnI 2012 ATTAAT AT^TAAT 2460 ATTAAT Asp700I 971 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1338 GAATTTTTTC AspHI 1140 GTGCAC G[AT]GC[AT]^C 1669 GAGCAC BcnI 455 CCCGG CC^[GC]GG 511 CCGGG BlpI 868 GCTCAGC GC^T[ACGT]AGC 877 GCTCAGC BmyI 1140 GTGCAC G[AGT]GC[ACT]^C 1669 GAGCAC Bpu1102I 868 GCTCAGC GC^T[ACGT]AGC 877 GCTCAGC BsiHKAI 1140 GTGCAC G[AT]GC[AT]^C 1669 GAGCAC Bsp1286I 1140 GTGCAC G[AGT]GC[ACT]^C 1669 GAGCAC BspHI 663 TCATGA T^CATGA 1854 TCATGA BspMI 858 ACCTGC ACCTGC(4/8) 1731 ACCTGC Bsu36I 309 CCTCAGG CC^T[ACGT]AGG 1748 CCTGAGG CelII 868 GCTCAGC GC^T[ACGT]AGC 877 GCTCAGC CfoI 99 GCGC GCG^C 393 GCGC DraII 1909 AGGGCCT [GA]G^G[ACGT]CC[CT] 2371 AGGCCCT DsaI 1543 CCACGG C^C[GA][CT]GG 1959 CCATGG Eco81I 309 CCTCAGG CC^T[ACGT]AGG 1748 CCTGAGG EcoNI 976 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1744 CCTTCCTGAGG EcoO109I 1909 AGGGCCT [GA]G^G[ACGT]CC[CT] 2371 AGGCCCT HapII 456 CCGG C^CGG 511 CCGG HgaI 365 GACGC GACGC(5/10) 667 GACGC HhaI 99 GCGC GCG^C 393 GCGC HinP1I 99 GCGC G^CGC 393 GCGC HpaII 456 CCGG C^CGG 511 CCGG MspA1I 905 CAGCTG C[AC]G^C[GT]G 2553 CAGCTG MspI 456 CCGG C^CGG 511 CCGG NciI 455 CCCGG CC^[GC]GG 511 CCGGG PshBI 2012 ATTAAT AT^TAAT 2460 ATTAAT PstI 1637 CTGCAG CTGCA^G 1837 CTGCAG PvuII 905 CAGCTG CAG^CTG 2553 CAGCTG RcaI 663 TCATGA T^CATGA 1854 TCATGA RsaI 272 GTAC GT^AC 1761 GTAC SfcI 1637 CTGCAG C^T[GA][CT]AG 1837 CTGCAG XmnI 971 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1338 GAATTTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AcsI AluI AlwI ApoI AvaII BbvI BfaI BpmI BsaJI BseRI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII