##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AlwNI ApaI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I EcoRV EcoT22I
FauI FbaI FseI FspI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 422 GTCGAC GT^[AC][GT]AC
AccII 1621 CGCG CG^CG
AcyI 365 GACGCC G[GA]^CG[CT]C
Alw44I 1140 GTGCAC G^TGCAC
Aor51HI 392 AGCGCT AGC^GCT
ApaLI 1140 GTGCAC G^TGCAC
BbvCI 482 GCTGAGG CCTCAGC(-5/-2)
BsaBI 2046 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 365 GACGCC G[GA]^CG[CT]C
BsaI 1487 GGTCTC GGTCTC(1/5)
BsgI 997 CTGCAC GTGCAG(16/14)
BsmAI 1488 GTCTC GTCTC(1/5)
BsrDI 477 GCAATG GCAATG(2/0)
BstEII 1691 GGTCACC G^GT[ACGT]ACC
BstPI 1691 GGTCACC G^GT[ACGT]ACC
BstUI 1621 CGCG CG^CG
BstXI 1543 CCACGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 440 CGGCCA [CT]^GGCC[GA]
Eco47III 392 AGCGCT AGC^GCT
EcoO65I 1691 GGTCACC G^GT[ACGT]ACC
EcoRI 685 GAATTC G^AATTC
HaeII 392 AGCGCT [GA]GCGC^[CT]
Hin1I 365 GACGCC G[GA]^CG[CT]C
HindIII 207 AAGCTT A^AGCTT
HpaI 1390 GTTAAC GTT^AAC
MamI 2046 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1621 CGCG CG^CG
NcoI 1959 CCATGG C^CATGG
NspI 873 GCATGC [GA]CATG^[CT]
SalI 422 GTCGAC G^TCGAC
SphI 873 GCATGC GCATG^C
SspI 2528 AATATT AAT^ATT
SwaI 2417 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AciI 529 GCGG CCGC(-3/-1)
733 GCGG
AfaI 272 GTAC GT^AC
1761 GTAC
AocI 309 CCTCAGG CC^T[ACGT]AGG
1748 CCTGAGG
AseI 2012 ATTAAT AT^TAAT
2460 ATTAAT
AsnI 2012 ATTAAT AT^TAAT
2460 ATTAAT
Asp700I 971 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1338 GAATTTTTTC
AspHI 1140 GTGCAC G[AT]GC[AT]^C
1669 GAGCAC
BcnI 455 CCCGG CC^[GC]GG
511 CCGGG
BlpI 868 GCTCAGC GC^T[ACGT]AGC
877 GCTCAGC
BmyI 1140 GTGCAC G[AGT]GC[ACT]^C
1669 GAGCAC
Bpu1102I 868 GCTCAGC GC^T[ACGT]AGC
877 GCTCAGC
BsiHKAI 1140 GTGCAC G[AT]GC[AT]^C
1669 GAGCAC
Bsp1286I 1140 GTGCAC G[AGT]GC[ACT]^C
1669 GAGCAC
BspHI 663 TCATGA T^CATGA
1854 TCATGA
BspMI 858 ACCTGC ACCTGC(4/8)
1731 ACCTGC
Bsu36I 309 CCTCAGG CC^T[ACGT]AGG
1748 CCTGAGG
CelII 868 GCTCAGC GC^T[ACGT]AGC
877 GCTCAGC
CfoI 99 GCGC GCG^C
393 GCGC
DraII 1909 AGGGCCT [GA]G^G[ACGT]CC[CT]
2371 AGGCCCT
DsaI 1543 CCACGG C^C[GA][CT]GG
1959 CCATGG
Eco81I 309 CCTCAGG CC^T[ACGT]AGG
1748 CCTGAGG
EcoNI 976 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1744 CCTTCCTGAGG
EcoO109I 1909 AGGGCCT [GA]G^G[ACGT]CC[CT]
2371 AGGCCCT
HapII 456 CCGG C^CGG
511 CCGG
HgaI 365 GACGC GACGC(5/10)
667 GACGC
HhaI 99 GCGC GCG^C
393 GCGC
HinP1I 99 GCGC G^CGC
393 GCGC
HpaII 456 CCGG C^CGG
511 CCGG
MspA1I 905 CAGCTG C[AC]G^C[GT]G
2553 CAGCTG
MspI 456 CCGG C^CGG
511 CCGG
NciI 455 CCCGG CC^[GC]GG
511 CCGGG
PshBI 2012 ATTAAT AT^TAAT
2460 ATTAAT
PstI 1637 CTGCAG CTGCA^G
1837 CTGCAG
PvuII 905 CAGCTG CAG^CTG
2553 CAGCTG
RcaI 663 TCATGA T^CATGA
1854 TCATGA
RsaI 272 GTAC GT^AC
1761 GTAC
SfcI 1637 CTGCAG C^T[GA][CT]AG
1837 CTGCAG
XmnI 971 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1338 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BfaI BpmI BsaJI BseRI
BsiYI BslI BsmFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EcoRII EcoT14I
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII