################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AviII AvrII BalI BbrPI BbsI BbvCI BcgI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiWI BspDI BspEI BspLU11I BspMI BsrFI BssHII Bst1107I BstBI BstXI BstZ17I Cfr10I ClaI CpoI DraII DraIII Eco47III EcoNI EcoO109I EcoRI FseI FspI HpaI KpnI MamI MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NruI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 70 enzymes) # ################################### AatII 1989 GACGTC GACGT^C AccI 664 GTCTAC GT^[AC][GT]AC AflIII 1047 ACACGT A^C[GA][CT]GT AhdI 1238 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 333 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 211 CCTCAGG CC^T[ACGT]AGG AseI 926 ATTAAT AT^TAAT AsnI 926 ATTAAT AT^TAAT Asp700I 2004 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1238 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1940 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 169 CCCGGG C^[CT]CG[GA]G BamHI 1406 GGATCC G^GATCC BbeI 1759 GGCGCC GGCGC^C BciVI 2034 GTATCC GTATCC(6/5) BclI 1011 TGATCA T^GATCA BglI 12 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 269 GCTGAGC GC^T[ACGT]AGC Bpu1102I 269 GCTGAGC GC^T[ACGT]AGC BsaI 286 GAGACC GGTCTC(1/5) BsoBI 169 CCCGGG C^[CT]CG[GA]G Bsp1407I 1056 TGTACA T^GTACA BspHI 1398 TCATGA T^CATGA BsrDI 1618 CATTGC GCAATG(2/0) BsrGI 1056 TGTACA T^GTACA BstAPI 1677 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 211 CCTCAGG CC^T[ACGT]AGG CelII 269 GCTGAGC GC^T[ACGT]AGC DraI 2081 TTTAAA TTT^AAA DrdI 1388 GACAGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 19 CGGCCG [CT]^GGCC[GA] EagI 19 CGGCCG C^GGCCG Eam1105I 1238 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 19 CGGCCG C^GGCCG Eco52I 19 CGGCCG C^GGCCG Eco81I 211 CCTCAGG CC^T[ACGT]AGG EcoRV 974 GATATC GAT^ATC EcoT22I 1837 ATGCAT ATGCA^T FauI 1450 GCGGG CCCGC(4/6) FbaI 1011 TGATCA T^GATCA HaeII 1759 GGCGCC [GA]GCGC^[CT] HgaI 128 GACGC GACGC(5/10) HincII 1556 GTTGAC GT[CT]^[GA]AC HindII 1556 GTTGAC GT[CT]^[GA]AC HindIII 592 AAGCTT A^AGCTT KasI 1759 GGCGCC G^GCGCC KspI 16 CCGCGG CCGC^GG NarI 1759 GGCGCC GG^CGCC NcoI 804 CCATGG C^CATGG NheI 1864 GCTAGC G^CTAGC NotI 18 GCGGCCGC GC^GGCCGC NsiI 1837 ATGCAT ATGCA^T PflFI 1940 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 2143 GACTC GAGTC(4/5) PshBI 926 ATTAAT AT^TAAT PvuII 1039 CAGCTG CAG^CTG RcaI 1398 TCATGA T^CATGA SacI 998 GAGCTC GAGCT^C SacII 16 CCGCGG CCGC^GG SapI 995 GAAGAGC GCTCTTC(1/4) SexAI 1296 ACCAGGT A^CC[AT]GGT SfoI 1759 GGCGCC GGC^GCC SmaI 169 CCCGGG CCC^GGG SspBI 1056 TGTACA T^GTACA TfiI 1720 GAATC G^A[AT]TC Tth111I 1940 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 804 CCATGGATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 169 CCCGGG C^CCGGG XmaI 169 CCCGGG C^CCGGG XmnI 2004 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 115 GAGCAC G[AT]GC[AT]^C 998 GAGCTC BpmI 339 CTGGAG CTGGAG(16/14) 444 CTGGAG BsiHKAI 115 GAGCAC G[AT]GC[AT]^C 998 GAGCTC BsmBI 95 CGTCTC CGTCTC(1/5) 121 GAGACG BsrBI 161 CCGCTC CCGCTC(-3/-3) 1745 GAGCGG BssSI 118 CACGAG CACGAG(-5/-1) 1473 CACGAG BstEII 308 GGTCACC G^GT[ACGT]ACC 630 GGTTACC BstPI 308 GGTCACC G^GT[ACGT]ACC 630 GGTTACC EcoO65I 308 GGTCACC G^GT[ACGT]ACC 630 GGTTACC EcoT14I 804 CCATGG C^C[AT][AT]GG 1605 CCAAGG HinfI 1720 GAATC G^A[ACGT]TC 2143 GACTC PstI 1020 CTGCAG CTGCA^G 1684 CTGCAG SspI 838 AATATT AAT^ATT 965 AATATT StyI 804 CCATGG C^C[AT][AT]GG 1605 CCAAGG TaqI 242 TCGA T^CGA 1434 TCGA TthHB8I 242 TCGA T^CGA 1434 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AvaII BanI BanII BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI XhoII