##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AviII AvrII BalI BbrPI
BbsI BbvCI BcgI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsgI BsiWI BspDI BspEI
BspLU11I BspMI BsrFI BssHII Bst1107I
BstBI BstXI BstZ17I Cfr10I ClaI
CpoI DraII DraIII Eco47III EcoNI
EcoO109I EcoRI FseI FspI HpaI
KpnI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NdeI
NgoMIV NruI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 70 enzymes) #
###################################
AatII 1989 GACGTC GACGT^C
AccI 664 GTCTAC GT^[AC][GT]AC
AflIII 1047 ACACGT A^C[GA][CT]GT
AhdI 1238 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 333 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 211 CCTCAGG CC^T[ACGT]AGG
AseI 926 ATTAAT AT^TAAT
AsnI 926 ATTAAT AT^TAAT
Asp700I 2004 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1238 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1940 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 169 CCCGGG C^[CT]CG[GA]G
BamHI 1406 GGATCC G^GATCC
BbeI 1759 GGCGCC GGCGC^C
BciVI 2034 GTATCC GTATCC(6/5)
BclI 1011 TGATCA T^GATCA
BglI 12 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 269 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 269 GCTGAGC GC^T[ACGT]AGC
BsaI 286 GAGACC GGTCTC(1/5)
BsoBI 169 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1056 TGTACA T^GTACA
BspHI 1398 TCATGA T^CATGA
BsrDI 1618 CATTGC GCAATG(2/0)
BsrGI 1056 TGTACA T^GTACA
BstAPI 1677 GCATTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 211 CCTCAGG CC^T[ACGT]AGG
CelII 269 GCTGAGC GC^T[ACGT]AGC
DraI 2081 TTTAAA TTT^AAA
DrdI 1388 GACAGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 19 CGGCCG [CT]^GGCC[GA]
EagI 19 CGGCCG C^GGCCG
Eam1105I 1238 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 19 CGGCCG C^GGCCG
Eco52I 19 CGGCCG C^GGCCG
Eco81I 211 CCTCAGG CC^T[ACGT]AGG
EcoRV 974 GATATC GAT^ATC
EcoT22I 1837 ATGCAT ATGCA^T
FauI 1450 GCGGG CCCGC(4/6)
FbaI 1011 TGATCA T^GATCA
HaeII 1759 GGCGCC [GA]GCGC^[CT]
HgaI 128 GACGC GACGC(5/10)
HincII 1556 GTTGAC GT[CT]^[GA]AC
HindII 1556 GTTGAC GT[CT]^[GA]AC
HindIII 592 AAGCTT A^AGCTT
KasI 1759 GGCGCC G^GCGCC
KspI 16 CCGCGG CCGC^GG
NarI 1759 GGCGCC GG^CGCC
NcoI 804 CCATGG C^CATGG
NheI 1864 GCTAGC G^CTAGC
NotI 18 GCGGCCGC GC^GGCCGC
NsiI 1837 ATGCAT ATGCA^T
PflFI 1940 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2143 GACTC GAGTC(4/5)
PshBI 926 ATTAAT AT^TAAT
PvuII 1039 CAGCTG CAG^CTG
RcaI 1398 TCATGA T^CATGA
SacI 998 GAGCTC GAGCT^C
SacII 16 CCGCGG CCGC^GG
SapI 995 GAAGAGC GCTCTTC(1/4)
SexAI 1296 ACCAGGT A^CC[AT]GGT
SfoI 1759 GGCGCC GGC^GCC
SmaI 169 CCCGGG CCC^GGG
SspBI 1056 TGTACA T^GTACA
TfiI 1720 GAATC G^A[AT]TC
Tth111I 1940 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 804 CCATGGATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 169 CCCGGG C^CCGGG
XmaI 169 CCCGGG C^CCGGG
XmnI 2004 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspHI 115 GAGCAC G[AT]GC[AT]^C
998 GAGCTC
BpmI 339 CTGGAG CTGGAG(16/14)
444 CTGGAG
BsiHKAI 115 GAGCAC G[AT]GC[AT]^C
998 GAGCTC
BsmBI 95 CGTCTC CGTCTC(1/5)
121 GAGACG
BsrBI 161 CCGCTC CCGCTC(-3/-3)
1745 GAGCGG
BssSI 118 CACGAG CACGAG(-5/-1)
1473 CACGAG
BstEII 308 GGTCACC G^GT[ACGT]ACC
630 GGTTACC
BstPI 308 GGTCACC G^GT[ACGT]ACC
630 GGTTACC
EcoO65I 308 GGTCACC G^GT[ACGT]ACC
630 GGTTACC
EcoT14I 804 CCATGG C^C[AT][AT]GG
1605 CCAAGG
HinfI 1720 GAATC G^A[ACGT]TC
2143 GACTC
PstI 1020 CTGCAG CTGCA^G
1684 CTGCAG
SspI 838 AATATT AAT^ATT
965 AATATT
StyI 804 CCATGG C^C[AT][AT]GG
1605 CCAAGG
TaqI 242 TCGA T^CGA
1434 TCGA
TthHB8I 242 TCGA T^CGA
1434 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaII BanI
BanII BbvI BcnI BfaI BmyI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII