################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BbeI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI Eco47III EcoT22I FbaI FseI FspI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SbfI SfiI SfoI SfuI SgrAI SmaI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 1955 GTCGAC GT^[AC][GT]AC AccII 68 CGCG CG^CG AhdI 58 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 60 CCTGAGG CC^T[ACGT]AGG ApaI 453 GGGCCC GGGCC^C AspEI 58 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 305 GGATCC G^GATCC BanI 443 GGCACC G^G[CT][GA]CC BbrPI 448 CACGTG CAC^GTG BsaI 369 GAGACC GGTCTC(1/5) BsaWI 2077 ACCGGA [AT]^CCGG[AT] BsiWI 2456 CGTACG C^GTACG BspMI 1475 GCAGGT ACCTGC(4/8) BstAPI 2015 GCATTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1419 GGTGACC G^GT[ACGT]ACC BstPI 1419 GGTGACC G^GT[ACGT]ACC BstUI 68 CGCG CG^CG BstYI 305 GGATCC [GA]^GATC[CT] Bsu36I 60 CCTGAGG CC^T[ACGT]AGG DrdI 602 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 574 CCATGG C^C[GA][CT]GG Eam1105I 58 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 60 CCTGAGG CC^T[ACGT]AGG EcoNI 1469 CCTTTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1419 GGTGACC G^GT[ACGT]ACC HaeII 43 GGCGCT [GA]GCGC^[CT] HgaI 2070 GACGC GACGC(5/10) HincII 1955 GTCGAC GT[CT]^[GA]AC HindII 1955 GTCGAC GT[CT]^[GA]AC MflI 305 GGATCC [GA]^GATC[CT] MspA1I 38 CAGCGG C[AC]G^C[GT]G MvnI 68 CGCG CG^CG NcoI 574 CCATGG C^CATGG PmaCI 448 CACGTG CAC^GTG PmlI 448 CACGTG CAC^GTG SalI 1955 GTCGAC G^TCGAC ScaI 14 AGTACT AGT^ACT SexAI 506 ACCTGGT A^CC[AT]GGT SmlI 1661 CTCAAG C^T[CT][GA]AG SnaBI 2454 TACGTA TAC^GTA SpeI 942 ACTAGT A^CTAGT XbaI 801 TCTAGA T^CTAGA XhoII 305 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 897 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG 1810 CAGTCCCTG BbvCI 321 CCTCAGC CCTCAGC(-5/-2) 649 CCTCAGC BcgI 136 CGACCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 254 CGAAGTGGCTGC BglI 81 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 252 GCCGAAGTGGC BsaAI 448 CACGTG [CT]AC^GT[GA] 2454 TACGTA BsiEI 250 CGGCCG CG[GA][CT]^CG 2294 CGGCCG BssSI 1036 CACGAG CACGAG(-5/-1) 1978 CACGAG CfoI 44 GCGC GCG^C 1308 GCGC DraI 1867 TTTAAA TTT^AAA 2404 TTTAAA DraIII 445 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 505 CACCTGGTG EagI 250 CGGCCG C^GGCCG 2294 CGGCCG EclXI 250 CGGCCG C^GGCCG 2294 CGGCCG Eco52I 250 CGGCCG C^GGCCG 2294 CGGCCG EcoRV 579 GATATC GAT^ATC 712 GATATC HhaI 44 GCGC GCG^C 1308 GCGC HinP1I 44 GCGC G^CGC 1308 GCGC HindIII 1862 AAGCTT A^AGCTT 2499 AAGCTT MslI 1108 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1699 CATTGTAATG NdeI 1582 CATATG CA^TATG 2463 CATATG PpuMI 1504 AGGACCC [GA]G^G[AT]CC[CT] 1984 AGGACCC PshAI 1720 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1958 GACTCTCGTC SacI 1085 GAGCTC GAGCT^C 2211 GAGCTC SapI 211 GAAGAGC GCTCTTC(1/4) 568 GAAGAGC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI