##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BalI BbeI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
Eco47III EcoT22I FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SbfI
SfiI SfoI SfuI SgrAI SmaI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1955 GTCGAC GT^[AC][GT]AC
AccII 68 CGCG CG^CG
AhdI 58 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 60 CCTGAGG CC^T[ACGT]AGG
ApaI 453 GGGCCC GGGCC^C
AspEI 58 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 305 GGATCC G^GATCC
BanI 443 GGCACC G^G[CT][GA]CC
BbrPI 448 CACGTG CAC^GTG
BsaI 369 GAGACC GGTCTC(1/5)
BsaWI 2077 ACCGGA [AT]^CCGG[AT]
BsiWI 2456 CGTACG C^GTACG
BspMI 1475 GCAGGT ACCTGC(4/8)
BstAPI 2015 GCATTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1419 GGTGACC G^GT[ACGT]ACC
BstPI 1419 GGTGACC G^GT[ACGT]ACC
BstUI 68 CGCG CG^CG
BstYI 305 GGATCC [GA]^GATC[CT]
Bsu36I 60 CCTGAGG CC^T[ACGT]AGG
DrdI 602 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 574 CCATGG C^C[GA][CT]GG
Eam1105I 58 GACCTGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 60 CCTGAGG CC^T[ACGT]AGG
EcoNI 1469 CCTTTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1419 GGTGACC G^GT[ACGT]ACC
HaeII 43 GGCGCT [GA]GCGC^[CT]
HgaI 2070 GACGC GACGC(5/10)
HincII 1955 GTCGAC GT[CT]^[GA]AC
HindII 1955 GTCGAC GT[CT]^[GA]AC
MflI 305 GGATCC [GA]^GATC[CT]
MspA1I 38 CAGCGG C[AC]G^C[GT]G
MvnI 68 CGCG CG^CG
NcoI 574 CCATGG C^CATGG
PmaCI 448 CACGTG CAC^GTG
PmlI 448 CACGTG CAC^GTG
SalI 1955 GTCGAC G^TCGAC
ScaI 14 AGTACT AGT^ACT
SexAI 506 ACCTGGT A^CC[AT]GGT
SmlI 1661 CTCAAG C^T[CT][GA]AG
SnaBI 2454 TACGTA TAC^GTA
SpeI 942 ACTAGT A^CTAGT
XbaI 801 TCTAGA T^CTAGA
XhoII 305 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 897 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
1810 CAGTCCCTG
BbvCI 321 CCTCAGC CCTCAGC(-5/-2)
649 CCTCAGC
BcgI 136 CGACCATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
254 CGAAGTGGCTGC
BglI 81 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
252 GCCGAAGTGGC
BsaAI 448 CACGTG [CT]AC^GT[GA]
2454 TACGTA
BsiEI 250 CGGCCG CG[GA][CT]^CG
2294 CGGCCG
BssSI 1036 CACGAG CACGAG(-5/-1)
1978 CACGAG
CfoI 44 GCGC GCG^C
1308 GCGC
DraI 1867 TTTAAA TTT^AAA
2404 TTTAAA
DraIII 445 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
505 CACCTGGTG
EagI 250 CGGCCG C^GGCCG
2294 CGGCCG
EclXI 250 CGGCCG C^GGCCG
2294 CGGCCG
Eco52I 250 CGGCCG C^GGCCG
2294 CGGCCG
EcoRV 579 GATATC GAT^ATC
712 GATATC
HhaI 44 GCGC GCG^C
1308 GCGC
HinP1I 44 GCGC G^CGC
1308 GCGC
HindIII 1862 AAGCTT A^AGCTT
2499 AAGCTT
MslI 1108 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1699 CATTGTAATG
NdeI 1582 CATATG CA^TATG
2463 CATATG
PpuMI 1504 AGGACCC [GA]G^G[AT]CC[CT]
1984 AGGACCC
PshAI 1720 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1958 GACTCTCGTC
SacI 1085 GAGCTC GAGCT^C
2211 GAGCTC
SapI 211 GAAGAGC GCTCTTC(1/4)
568 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI