################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccI AccII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII BbeI BcgI BciVI BfrI BglI BglII BsiWI BspDI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I ClaI CpoI DraI DsaI Eam1105I EcoO65I FseI FspI HpaI KasI KspI MfeI MluI MunI MvnI NarI NcoI NdeI NheI NruI NspV PacI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 1375 GGTACC G^GTACC AccIII 504 TCCGGA T^CCGGA AcyI 1134 GACGCC G[GA]^CG[CT]C Aor51HI 53 AGCGCT AGC^GCT Asp700I 1521 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1375 GGTACC G^GTACC AspI 114 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2176 CTCGAG C^[CT]CG[GA]G AvrII 432 CCTAGG C^CTAGG BamHI 100 GGATCC G^GATCC BbrPI 796 CACGTG CAC^GTG BbvCI 897 GCTGAGG CCTCAGC(-5/-2) BlnI 432 CCTAGG C^CTAGG BlpI 819 GCTGAGC GC^T[ACGT]AGC Bpu1102I 819 GCTGAGC GC^T[ACGT]AGC BsaAI 796 CACGTG [CT]AC^GT[GA] BsaBI 526 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1134 GACGCC G[GA]^CG[CT]C BsaI 2475 GAGACC GGTCTC(1/5) BseAI 504 TCCGGA T^CCGGA BsiEI 46 CGGCCG CG[GA][CT]^CG BsoBI 2176 CTCGAG C^[CT]CG[GA]G Bsp1407I 1001 TGTACA T^GTACA BspEI 504 TCCGGA T^CCGGA BsrGI 1001 TGTACA T^GTACA CelII 819 GCTGAGC GC^T[ACGT]AGC DraIII 2237 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 46 CGGCCG C^GGCCG EclXI 46 CGGCCG C^GGCCG Eco47III 53 AGCGCT AGC^GCT Eco52I 46 CGGCCG C^GGCCG EcoRI 1532 GAATTC G^AATTC EcoRV 1734 GATATC GAT^ATC EcoT22I 342 ATGCAT ATGCA^T Hin1I 1134 GACGCC G[GA]^CG[CT]C HindIII 652 AAGCTT A^AGCTT KpnI 1375 GGTACC GGTAC^C MaeII 797 ACGT A^CGT MamI 526 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 504 TCCGGA T^CCGGA NaeI 1680 GCCGGC GCC^GGC NgoMIV 1680 GCCGGC G^CCGGC NotI 45 GCGGCCGC GC^GGCCGC NsiI 342 ATGCAT ATGCA^T PaeR7I 2176 CTCGAG C^TCGAG PflFI 114 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 796 CACGTG CAC^GTG PmlI 796 CACGTG CAC^GTG SacI 2651 GAGCTC GAGCT^C SapI 618 GAAGAGC GCTCTTC(1/4) SbfI 867 CCTGCAGG CCTGCA^GG Sse8387I 867 CCTGCAGG CCTGCA^GG SspBI 1001 TGTACA T^GTACA Tth111I 114 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2176 CTCGAG C^TCGAG XmnI 1521 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 720 GAATTT [GA]^AATT[CT] 1532 GAATTC AocI 685 CCTGAGG CC^T[ACGT]AGG 1166 CCTTAGG ApaI 1559 GGGCCC GGGCC^C 2593 GGGCCC ApoI 720 GAATTT [GA]^AATT[CT] 1532 GAATTC BalI 380 TGGCCA TGG^CCA 1566 TGGCCA BseRI 640 GAGGAG GAGGAG(10/8) 2059 GAGGAG BsmBI 605 CGTCTC CGTCTC(1/5) 1132 GAGACG BsmI 1081 GCATTC GAATGC(1/-1) 1705 GCATTC BsrFI 707 ACCGGC [GA]^CCGG[CT] 1680 GCCGGC Bsu36I 685 CCTGAGG CC^T[ACGT]AGG 1166 CCTTAGG CfoI 54 GCGC GCG^C 151 GCGC Cfr10I 707 ACCGGC [GA]^CCGG[CT] 1680 GCCGGC DrdI 1855 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1894 GACTCTTCAGTC Eco81I 685 CCTGAGG CC^T[ACGT]AGG 1166 CCTTAGG FauI 1069 CCCGC CCCGC(4/6) 91 GCGGG HaeII 53 AGCGCT [GA]GCGC^[CT] 150 GGCGCT HhaI 54 GCGC GCG^C 151 GCGC HinP1I 54 GCGC G^CGC 151 GCGC HincII 1593 GTTGAC GT[CT]^[GA]AC 2156 GTTGAC HindII 1593 GTTGAC GT[CT]^[GA]AC 2156 GTTGAC MluNI 380 TGGCCA TGG^CCA 1566 TGGCCA MscI 380 TGGCCA TGG^CCA 1566 TGGCCA MspA1I 89 CAGCGG C[AC]G^C[GT]G 143 CAGCGG TaqI 1754 TCGA T^CGA 2177 TCGA TthHB8I 1754 TCGA T^CGA 2177 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII