##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AviII
BbeI BcgI BciVI BfrI BglI
BglII BsiWI BspDI BspHI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI DsaI
Eam1105I EcoO65I FseI FspI HpaI
KasI KspI MfeI MluI MunI
MvnI NarI NcoI NdeI NheI
NruI NspV PacI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI Van91I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1375 GGTACC G^GTACC
AccIII 504 TCCGGA T^CCGGA
AcyI 1134 GACGCC G[GA]^CG[CT]C
Aor51HI 53 AGCGCT AGC^GCT
Asp700I 1521 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1375 GGTACC G^GTACC
AspI 114 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2176 CTCGAG C^[CT]CG[GA]G
AvrII 432 CCTAGG C^CTAGG
BamHI 100 GGATCC G^GATCC
BbrPI 796 CACGTG CAC^GTG
BbvCI 897 GCTGAGG CCTCAGC(-5/-2)
BlnI 432 CCTAGG C^CTAGG
BlpI 819 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 819 GCTGAGC GC^T[ACGT]AGC
BsaAI 796 CACGTG [CT]AC^GT[GA]
BsaBI 526 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1134 GACGCC G[GA]^CG[CT]C
BsaI 2475 GAGACC GGTCTC(1/5)
BseAI 504 TCCGGA T^CCGGA
BsiEI 46 CGGCCG CG[GA][CT]^CG
BsoBI 2176 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1001 TGTACA T^GTACA
BspEI 504 TCCGGA T^CCGGA
BsrGI 1001 TGTACA T^GTACA
CelII 819 GCTGAGC GC^T[ACGT]AGC
DraIII 2237 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 46 CGGCCG C^GGCCG
EclXI 46 CGGCCG C^GGCCG
Eco47III 53 AGCGCT AGC^GCT
Eco52I 46 CGGCCG C^GGCCG
EcoRI 1532 GAATTC G^AATTC
EcoRV 1734 GATATC GAT^ATC
EcoT22I 342 ATGCAT ATGCA^T
Hin1I 1134 GACGCC G[GA]^CG[CT]C
HindIII 652 AAGCTT A^AGCTT
KpnI 1375 GGTACC GGTAC^C
MaeII 797 ACGT A^CGT
MamI 526 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 504 TCCGGA T^CCGGA
NaeI 1680 GCCGGC GCC^GGC
NgoMIV 1680 GCCGGC G^CCGGC
NotI 45 GCGGCCGC GC^GGCCGC
NsiI 342 ATGCAT ATGCA^T
PaeR7I 2176 CTCGAG C^TCGAG
PflFI 114 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 796 CACGTG CAC^GTG
PmlI 796 CACGTG CAC^GTG
SacI 2651 GAGCTC GAGCT^C
SapI 618 GAAGAGC GCTCTTC(1/4)
SbfI 867 CCTGCAGG CCTGCA^GG
Sse8387I 867 CCTGCAGG CCTGCA^GG
SspBI 1001 TGTACA T^GTACA
Tth111I 114 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2176 CTCGAG C^TCGAG
XmnI 1521 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 720 GAATTT [GA]^AATT[CT]
1532 GAATTC
AocI 685 CCTGAGG CC^T[ACGT]AGG
1166 CCTTAGG
ApaI 1559 GGGCCC GGGCC^C
2593 GGGCCC
ApoI 720 GAATTT [GA]^AATT[CT]
1532 GAATTC
BalI 380 TGGCCA TGG^CCA
1566 TGGCCA
BseRI 640 GAGGAG GAGGAG(10/8)
2059 GAGGAG
BsmBI 605 CGTCTC CGTCTC(1/5)
1132 GAGACG
BsmI 1081 GCATTC GAATGC(1/-1)
1705 GCATTC
BsrFI 707 ACCGGC [GA]^CCGG[CT]
1680 GCCGGC
Bsu36I 685 CCTGAGG CC^T[ACGT]AGG
1166 CCTTAGG
CfoI 54 GCGC GCG^C
151 GCGC
Cfr10I 707 ACCGGC [GA]^CCGG[CT]
1680 GCCGGC
DrdI 1855 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACTCTTCAGTC
Eco81I 685 CCTGAGG CC^T[ACGT]AGG
1166 CCTTAGG
FauI 1069 CCCGC CCCGC(4/6)
91 GCGGG
HaeII 53 AGCGCT [GA]GCGC^[CT]
150 GGCGCT
HhaI 54 GCGC GCG^C
151 GCGC
HinP1I 54 GCGC G^CGC
151 GCGC
HincII 1593 GTTGAC GT[CT]^[GA]AC
2156 GTTGAC
HindII 1593 GTTGAC GT[CT]^[GA]AC
2156 GTTGAC
MluNI 380 TGGCCA TGG^CCA
1566 TGGCCA
MscI 380 TGGCCA TGG^CCA
1566 TGGCCA
MspA1I 89 CAGCGG C[AC]G^C[GT]G
143 CAGCGG
TaqI 1754 TCGA T^CGA
2177 TCGA
TthHB8I 1754 TCGA T^CGA
2177 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII