##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HgaI HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 384 GACGTC GACGT^C
AcyI 384 GACGTC G[GA]^CG[CT]C
ApaI 778 GGGCCC GGGCC^C
AspHI 943 GTGCTC G[AT]GC[AT]^C
AvaI 575 CTCGAG C^[CT]CG[GA]G
BalI 332 TGGCCA TGG^CCA
BanI 900 GGCACC G^G[CT][GA]CC
BbsI 1278 GAAGAC GAAGAC(2/6)
BglII 244 AGATCT A^GATCT
BpmI 50 CTGGAG CTGGAG(16/14)
BpuAI 1278 GAAGAC GAAGAC(2/6)
BsaAI 38 TACGTA [CT]AC^GT[GA]
BsaHI 384 GACGTC G[GA]^CG[CT]C
BsiEI 464 CGGCCG CG[GA][CT]^CG
BsiHKAI 943 GTGCTC G[AT]GC[AT]^C
BsmFI 476 GTCCC GGGAC(10/14)
BsoBI 575 CTCGAG C^[CT]CG[GA]G
BstBI 105 TTCGAA TT^CGAA
BstXI 191 CCAATCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1656 GCGC GCG^C
DsaI 608 CCATGG C^C[GA][CT]GG
EagI 464 CGGCCG C^GGCCG
EarI 1324 GAAGAG CTCTTC(1/4)
EclXI 464 CGGCCG C^GGCCG
Eco52I 464 CGGCCG C^GGCCG
EcoT14I 608 CCATGG C^C[AT][AT]GG
EcoT22I 1876 ATGCAT ATGCA^T
HapII 467 CCGG C^CGG
HhaI 1656 GCGC GCG^C
Hin1I 384 GACGTC G[GA]^CG[CT]C
HinP1I 1656 GCGC G^CGC
HindIII 1128 AAGCTT A^AGCTT
HpaII 467 CCGG C^CGG
Ksp632I 1324 GAAGAG CTCTTC(1/4)
MluNI 332 TGGCCA TGG^CCA
MscI 332 TGGCCA TGG^CCA
MspA1I 1157 CAGCTG C[AC]G^C[GT]G
MspI 467 CCGG C^CGG
NcoI 608 CCATGG C^CATGG
NsiI 1876 ATGCAT ATGCA^T
NspI 592 ACATGC [GA]CATG^[CT]
NspV 105 TTCGAA TT^CGAA
PaeR7I 575 CTCGAG C^TCGAG
PleI 1020 GAGTC GAGTC(4/5)
PvuII 1157 CAGCTG CAG^CTG
ScaI 1839 AGTACT AGT^ACT
SexAI 266 ACCTGGT A^CC[AT]GGT
SfcI 1584 CTGTAG C^T[GA][CT]AG
SfuI 105 TTCGAA TT^CGAA
SnaBI 38 TACGTA TAC^GTA
StuI 419 AGGCCT AGG^CCT
StyI 608 CCATGG C^C[AT][AT]GG
Tsp45I 1809 GTGAC ^GT[GC]AC
XhoI 575 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 375 GTAGAC GT^[AC][GT]AC
1586 GTAGAC
AlwI 325 GGATC GGATC(4/5)
1643 GGATC
AlwNI 291 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
833 CAGTTGCTG
BbvI 255 GCAGC GCAGC(8/12)
84 GCTGC
BlpI 838 GCTGAGC GC^T[ACGT]AGC
1159 GCTGAGC
Bpu1102I 838 GCTGAGC GC^T[ACGT]AGC
1159 GCTGAGC
BseRI 946 CTCCTC GAGGAG(10/8)
1085 CTCCTC
BstF5I 1902 CATCC GGATG(2/0)
19 GGATG
BstYI 244 AGATCT [GA]^GATC[CT]
325 GGATCT
CelII 838 GCTGAGC GC^T[ACGT]AGC
1159 GCTGAGC
DraII 777 GGGGCCC [GA]G^G[ACGT]CC[CT]
778 GGGCCCT
DrdI 378 GACCCCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1811 GACTTGATTGTC
EaeI 332 TGGCCA [CT]^GGCC[GA]
464 CGGCCG
EcoO109I 777 GGGGCCC [GA]G^G[ACGT]CC[CT]
778 GGGCCCT
FokI 1902 CATCC GGATG(9/13)
19 GGATG
MflI 244 AGATCT [GA]^GATC[CT]
325 GGATCT
MslI 753 CACCTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1869 CATTTTTATG
PflMI 1633 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1662 CCACTGCCTGG
SmlI 493 CTTGAG C^T[CT][GA]AG
575 CTCGAG
TseI 84 GCTGC G^C[AT]GC
255 GCAGC
Van91I 1633 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1662 CCACTGCCTGG
XhoII 244 AGATCT [GA]^GATC[CT]
325 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanII BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraI EcoRII Fnu4HI HaeIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SspI TaqI TfiI Tru9I Tsp509I
TspRI TthHB8I