##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AvrII
BamHI BbeI BbvCI BcgI BciVI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoRI FauI FseI HaeII
Hin1I HindIII KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 1418 GTAGAC GT^[AC][GT]AC
AcsI 1558 GAATTT [GA]^AATT[CT]
AfaI 1186 GTAC GT^AC
AflII 1785 CTTAAG C^TTAAG
AhdI 1722 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 531 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1047 CCTGAGG CC^T[ACGT]AGG
ApoI 1558 GAATTT [GA]^AATT[CT]
AspEI 1722 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1092 GAGCTC G[AT]GC[AT]^C
AvaII 1444 GGACC G^G[AT]CC
AviII 390 TGCGCA TGC^GCA
BalI 558 TGGCCA TGG^CCA
BanI 207 GGCACC G^G[CT][GA]CC
BanII 1092 GAGCTC G[GA]GC[CT]^C
BbrPI 377 CACGTG CAC^GTG
BclI 1581 TGATCA T^GATCA
BcnI 339 CCCGG CC^[GC]GG
BfrI 1785 CTTAAG C^TTAAG
BmyI 1092 GAGCTC G[AGT]GC[ACT]^C
BsaAI 377 CACGTG [CT]AC^GT[GA]
BseRI 1089 GAGGAG GAGGAG(10/8)
BsiHKAI 1092 GAGCTC G[AT]GC[AT]^C
BsmI 1630 GAATGC GAATGC(1/-1)
Bsp1286I 1092 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 1648 ACATGT A^CATGT
BstAPI 530 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1756 GGTGACC G^GT[ACGT]ACC
BstPI 1756 GGTGACC G^GT[ACGT]ACC
Bsu36I 1047 CCTGAGG CC^T[ACGT]AGG
Cac8I 405 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 391 GCGC GCG^C
EaeI 558 TGGCCA [CT]^GGCC[GA]
Eam1105I 1722 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1047 CCTGAGG CC^T[ACGT]AGG
EcoNI 241 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1756 GGTGACC G^GT[ACGT]ACC
EcoRV 293 GATATC GAT^ATC
EcoT22I 1204 ATGCAT ATGCA^T
FbaI 1581 TGATCA T^GATCA
FspI 390 TGCGCA TGC^GCA
HapII 340 CCGG C^CGG
HgaI 1710 GCGTC GACGC(5/10)
HhaI 391 GCGC GCG^C
HinP1I 391 GCGC G^CGC
HpaI 1966 GTTAAC GTT^AAC
HpaII 340 CCGG C^CGG
MluNI 558 TGGCCA TGG^CCA
MscI 558 TGGCCA TGG^CCA
MspI 340 CCGG C^CGG
NciI 339 CCCGG CC^[GC]GG
NsiI 1204 ATGCAT ATGCA^T
NspI 1648 ACATGT [GA]CATG^[CT]
PmaCI 377 CACGTG CAC^GTG
PmlI 377 CACGTG CAC^GTG
PshAI 1053 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1186 GTAC GT^AC
SacI 1092 GAGCTC GAGCT^C
SspI 1221 AATATT AAT^ATT
StuI 618 AGGCCT AGG^CCT
XbaI 1865 TCTAGA T^CTAGA
XcmI 920 CCAGCTGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 376 ACACGT A^C[GA][CT]GT
1648 ACATGT
AlwI 235 GGATC GGATC(4/5)
1383 GGATC
BbsI 838 GTCTTC GAAGAC(2/6)
1319 GTCTTC
BglII 1375 AGATCT A^GATCT
1848 AGATCT
BpuAI 838 GTCTTC GAAGAC(2/6)
1319 GTCTTC
BspHI 349 TCATGA T^CATGA
952 TCATGA
BstF5I 283 CATCC GGATG(2/0)
382 GGATG
DraI 40 TTTAAA TTT^AAA
2049 TTTAAA
DraII 1020 AGGCCCT [GA]G^G[ACGT]CC[CT]
1287 AGGCCCT
EcoO109I 1020 AGGCCCT [GA]G^G[ACGT]CC[CT]
1287 AGGCCCT
FokI 283 CATCC GGATG(9/13)
382 GGATG
HincII 334 GTCAAC GT[CT]^[GA]AC
1966 GTTAAC
HindII 334 GTCAAC GT[CT]^[GA]AC
1966 GTTAAC
MaeII 57 ACGT A^CGT
378 ACGT
MspA1I 531 CAGCTG C[AC]G^C[GT]G
921 CAGCTG
PvuII 531 CAGCTG CAG^CTG
921 CAGCTG
RcaI 349 TCATGA T^CATGA
952 TCATGA
TaqI 183 TCGA T^CGA
649 TCGA
TthHB8I 183 TCGA T^CGA
649 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AluI BbvI BfaI BpmI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstNI BstYI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII