##################################
# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaI
HpaII HphI KasI KpnI KspI
MaeII MaeIII MamI MfeI MluI
MluNI MroI MscI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI SexAI SfcI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SwaI TaqI
TfiI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AciI 136 GCGG CCGC(-3/-1)
AcsI 723 AAATTT [GA]^AATT[CT]
AfaI 508 GTAC GT^AC
ApoI 723 AAATTT [GA]^AATT[CT]
Asp700I 169 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 310 CCCGAG C^[CT]CG[GA]G
BanI 276 GGCACC G^G[CT][GA]CC
BbsI 570 GAAGAC GAAGAC(2/6)
BbvI 343 GCAGC GCAGC(8/12)
BglI 352 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 328 AGATCT A^GATCT
BpuAI 570 GAAGAC GAAGAC(2/6)
BsmAI 673 GTCTC GTCTC(1/5)
BsoBI 310 CCCGAG C^[CT]CG[GA]G
BsrDI 641 CATTGC GCAATG(2/0)
BsrI 586 CCAGT ACTGG(1/-1)
BstYI 328 AGATCT [GA]^GATC[CT]
Cfr13I 360 GGCCC G^G[ACGT]CC
DraI 776 TTTAAA TTT^AAA
DrdI 94 GACGATTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 137 CGGCCA [CT]^GGCC[GA]
EcoT14I 347 CCAAGG C^C[AT][AT]GG
MflI 328 AGATCT [GA]^GATC[CT]
MspA1I 265 CAGCTG C[AC]G^C[GT]G
NspI 562 GCATGC [GA]CATG^[CT]
PvuII 265 CAGCTG CAG^CTG
RsaI 508 GTAC GT^AC
SacI 148 GAGCTC GAGCT^C
Sau96I 360 GGCCC G^G[ACGT]CC
ScaI 507 AGTACT AGT^ACT
SfiI 351 GGCCAAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 562 GCATGC GCATG^C
SspI 256 AATATT AAT^ATT
StuI 334 AGGCCT AGG^CCT
StyI 347 CCAAGG C^C[AT][AT]GG
TseI 343 GCAGC G^C[AT]GC
XhoII 328 AGATCT [GA]^GATC[CT]
XmnI 169 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 117 GTGCTC G[AT]GC[AT]^C
148 GAGCTC
BanII 148 GAGCTC G[GA]GC[CT]^C
212 GAGCCC
BsiHKAI 117 GTGCTC G[AT]GC[AT]^C
148 GAGCTC
BssKI 395 CCTGG ^CC[ACGT]GG
421 CCAGG
BstF5I 426 GGATG GGATG(2/0)
447 GGATG
BstNI 395 CCTGG CC^[AT]GG
421 CCAGG
DpnII 246 GATC ^GATC
329 GATC
EarI 194 GAAGAG CTCTTC(1/4)
205 GAAGAG
EcoRII 395 CCTGG ^CC[AT]GG
421 CCAGG
Fnu4HI 136 GCGGC GC^[ACGT]GC
343 GCAGC
FokI 426 GGATG GGATG(9/13)
447 GGATG
HindIII 230 AAGCTT A^AGCTT
406 AAGCTT
ItaI 136 GCGGC GC^[ACGT]GC
343 GCAGC
Ksp632I 194 GAAGAG CTCTTC(1/4)
205 GAAGAG
MboI 246 GATC ^GATC
329 GATC
MslI 392 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
606 CATCTTCATG
MvaI 395 CCTGG CC^[AT]GG
421 CCAGG
NdeII 246 GATC ^GATC
329 GATC
SapI 194 GAAGAGC GCTCTTC(1/4)
205 GAAGAGC
Sau3AI 246 GATC ^GATC
329 GATC
ScrFI 395 CCTGG CC^[ACGT]GG
421 CCAGG
SfaNI 61 GATGC GCATC(5/9)
602 GCATC
TspRI 57 CACTG CA[GC]TG[ACGT][ACGT]^
587 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BfaI BmyI BsaJI Bsp1286I
Cac8I DdeI HaeIII MaeI MboII
MnlI MseI MwoI NlaIII NlaIV
Tru9I Tsp509I