##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BcgI BciVI BfaI
BfrI BglII BlnI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT22I FseI HgaI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 645 GACGTC GACGT^C
AfaI 521 GTAC GT^AC
AocI 1059 CCTGAGG CC^T[ACGT]AGG
AspHI 467 GTGCTC G[AT]GC[AT]^C
AvaI 462 CTCGGG C^[CT]CG[GA]G
AviII 76 TGCGCA TGC^GCA
BanII 1492 GGGCTC G[GA]GC[CT]^C
BbrPI 274 CACGTG CAC^GTG
BbsI 1168 GAAGAC GAAGAC(2/6)
BclI 833 TGATCA T^GATCA
BpmI 875 CTGGAG CTGGAG(16/14)
BpuAI 1168 GAAGAC GAAGAC(2/6)
BsaAI 274 CACGTG [CT]AC^GT[GA]
BsaWI 597 TCCGGT [AT]^CCGG[AT]
BseRI 1062 GAGGAG GAGGAG(10/8)
BsiHKAI 467 GTGCTC G[AT]GC[AT]^C
BsmI 816 GCATTC GAATGC(1/-1)
BsoBI 462 CTCGGG C^[CT]CG[GA]G
BspMI 1474 ACCTGC ACCTGC(4/8)
BsrDI 1116 CATTGC GCAATG(2/0)
Bsu36I 1059 CCTGAGG CC^T[ACGT]AGG
DrdI 1092 GACGGCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1406 CCGTGG C^C[GA][CT]GG
Eco81I 1059 CCTGAGG CC^T[ACGT]AGG
EcoRV 501 GATATC GAT^ATC
FbaI 833 TGATCA T^GATCA
FspI 76 TGCGCA TGC^GCA
PmaCI 274 CACGTG CAC^GTG
PmlI 274 CACGTG CAC^GTG
RsaI 521 GTAC GT^AC
SbfI 175 CCTGCAGG CCTGCA^GG
Sse8387I 175 CCTGCAGG CCTGCA^GG
StuI 1284 AGGCCT AGG^CCT
Tsp509I 714 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 69 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1426 CAGCGGCTG
AvaII 31 GGACC G^G[AT]CC
661 GGACC
BamHI 18 GGATCC G^GATCC
846 GGATCC
BbeI 558 GGCGCC GGCGC^C
1036 GGCGCC
BbvCI 289 CCTCAGC CCTCAGC(-5/-2)
1003 CCTCAGC
BcnI 41 CCCGG CC^[GC]GG
690 CCCGG
BglI 486 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1434 GCCACCCAGGC
BlpI 959 GCTTAGC GC^T[ACGT]AGC
1120 GCTGAGC
Bpu1102I 959 GCTTAGC GC^T[ACGT]AGC
1120 GCTGAGC
BsaI 142 GAGACC GGTCTC(1/5)
1471 GAGACC
BsmBI 236 CGTCTC CGTCTC(1/5)
647 CGTCTC
BssSI 537 CACGAG CACGAG(-5/-1)
1081 CACGAG
BstAPI 68 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1425 GCAGCGGCTGC
BstXI 657 CCAGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1400 CCAAGGCCGTGG
CelII 959 GCTTAGC GC^T[ACGT]AGC
1120 GCTGAGC
DraI 1568 TTTAAA TTT^AAA
1588 TTTAAA
EcoT14I 1400 CCAAGG C^C[AT][AT]GG
1488 CCTTGG
KasI 558 GGCGCC G^GCGCC
1036 GGCGCC
NarI 558 GGCGCC GG^CGCC
1036 GGCGCC
NciI 41 CCCGG CC^[GC]GG
690 CCCGG
PleI 1254 GACTC GAGTC(4/5)
259 GAGTC
PpuMI 30 GGGACCC [GA]G^G[AT]CC[CT]
660 GGGACCT
PstI 176 CTGCAG CTGCA^G
1558 CTGCAG
PvuII 215 CAGCTG CAG^CTG
1022 CAGCTG
SfoI 558 GGCGCC GGC^GCC
1036 GGCGCC
StyI 1400 CCAAGG C^C[AT][AT]GG
1488 CCTTGG
TfiI 137 GAATC G^A[AT]TC
1526 GAATC
Tsp45I 1294 GTGAC ^GT[GC]AC
1484 GTCAC
XcmI 945 CCAGGAGGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1479 CCATCGTCACCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AlwI BanI BbvI BmyI BsaHI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI Tru9I TseI TspRI
TthHB8I XhoII