##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccII AclI AflIII AgeI
Alw44I AlwNI Aor51HI ApaLI AscI
Asp718I AviII BalI BciVI BglI
BpmI BsaBI BsiEI BsiWI BspDI
BspHI BspLU11I BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I EcoO65I
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MluNI MscI
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflMI PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Van91I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 1848 GACGTC GACGT^C
AccIII 788 TCCGGA T^CCGGA
AflII 1210 CTTAAG C^TTAAG
AhdI 1378 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 473 CCTCAGG CC^T[ACGT]AGG
ApaI 718 GGGCCC GGGCC^C
AseI 1033 ATTAAT AT^TAAT
AsnI 1033 ATTAAT AT^TAAT
AspEI 1378 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2004 CCTAGG C^CTAGG
BbeI 1636 GGCGCC GGCGC^C
BbrPI 1987 CACGTG CAC^GTG
BbvCI 682 GCTGAGG CCTCAGC(-5/-2)
BcgI 1347 CGATTTATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 660 TGATCA T^GATCA
BcnI 581 CCGGG CC^[GC]GG
BfrI 1210 CTTAAG C^TTAAG
BlnI 2004 CCTAGG C^CTAGG
BlpI 1477 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1477 GCTGAGC GC^T[ACGT]AGC
BsaAI 1987 CACGTG [CT]AC^GT[GA]
BsaI 1982 GGTCTC GGTCTC(1/5)
BsaWI 788 TCCGGA [AT]^CCGG[AT]
BseAI 788 TCCGGA T^CCGGA
BsmBI 1846 GAGACG CGTCTC(1/5)
BspEI 788 TCCGGA T^CCGGA
Bst1107I 151 GTATAC GTA^TAC
BstZ17I 151 GTATAC GTA^TAC
Bsu36I 473 CCTCAGG CC^T[ACGT]AGG
CelII 1477 GCTGAGC GC^T[ACGT]AGC
DrdI 856 GACATCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1378 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 473 CCTCAGG CC^T[ACGT]AGG
EcoRI 2144 GAATTC G^AATTC
EcoRV 1444 GATATC GAT^ATC
EcoT22I 1061 ATGCAT ATGCA^T
FbaI 660 TGATCA T^GATCA
HgaI 58 GACGC GACGC(5/10)
HincII 739 GTTGAC GT[CT]^[GA]AC
HindII 739 GTTGAC GT[CT]^[GA]AC
HindIII 530 AAGCTT A^AGCTT
KasI 1636 GGCGCC G^GCGCC
MroI 788 TCCGGA T^CCGGA
NarI 1636 GGCGCC GG^CGCC
NciI 581 CCGGG CC^[GC]GG
NcoI 1867 CCATGG C^CATGG
NsiI 1061 ATGCAT ATGCA^T
NspI 458 ACATGC [GA]CATG^[CT]
PaeR7I 844 CTCGAG C^TCGAG
PmaCI 1987 CACGTG CAC^GTG
PmlI 1987 CACGTG CAC^GTG
PshBI 1033 ATTAAT AT^TAAT
PvuII 767 CAGCTG CAG^CTG
SacI 802 GAGCTC GAGCT^C
SfoI 1636 GGCGCC GGC^GCC
SspI 899 AATATT AAT^ATT
StuI 961 AGGCCT AGG^CCT
XhoI 844 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 151 GTATAC GT^[AC][GT]AC
511 GTAGAC
AcsI 1505 AAATTC [GA]^AATT[CT]
2144 GAATTC
AcyI 1636 GGCGCC G[GA]^CG[CT]C
1848 GACGTC
ApoI 1505 AAATTC [GA]^AATT[CT]
2144 GAATTC
Asp700I 3 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2109 GAATCATTTC
AspI 1098 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1831 GACTGAGTC
AvaI 844 CTCGAG C^[CT]CG[GA]G
1642 CCCGAG
BamHI 345 GGATCC G^GATCC
1359 GGATCC
BanI 91 GGCACC G^G[CT][GA]CC
1636 GGCGCC
BglII 113 AGATCT A^GATCT
2152 AGATCT
BsaHI 1636 GGCGCC G[GA]^CG[CT]C
1848 GACGTC
BseRI 471 CTCCTC GAGGAG(10/8)
199 GAGGAG
BsmI 3 GAATGC GAATGC(1/-1)
382 GAATGC
BsoBI 844 CTCGAG C^[CT]CG[GA]G
1642 CCCGAG
Bsp1407I 2029 TGTACA T^GTACA
2159 TGTACA
BspMI 252 ACCTGC ACCTGC(4/8)
1688 ACCTGC
BsrBI 562 CCGCTC CCGCTC(-3/-3)
1708 CCGCTC
BsrGI 2029 TGTACA T^GTACA
2159 TGTACA
CfoI 954 GCGC GCG^C
1637 GCGC
DraI 76 TTTAAA TTT^AAA
2179 TTTAAA
EaeI 578 TGGCCG [CT]^GGCC[GA]
1881 TGGCCG
EcoNI 723 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2004 CCTAGGAGAGG
HaeII 953 GGCGCT [GA]GCGC^[CT]
1636 GGCGCC
HhaI 954 GCGC GCG^C
1637 GCGC
Hin1I 1636 GGCGCC G[GA]^CG[CT]C
1848 GACGTC
HinP1I 954 GCGC G^CGC
1637 GCGC
MspA1I 767 CAGCTG C[AC]G^C[GT]G
1856 CAGCGG
PflFI 1098 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1831 GACTGAGTC
PpuMI 1369 GGGACCC [GA]G^G[AT]CC[CT]
1685 AGGACCT
SspBI 2029 TGTACA T^GTACA
2159 TGTACA
Tth111I 1098 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1831 GACTGAGTC
XmnI 3 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2109 GAATCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbsI BbvI BfaI
BmyI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII