##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI BspEI BsrBI BssHII
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FbaI FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflFI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SmaI SnaBI
Sse8387I StuI SwaI Tth111I XbaI
XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcyI 1656 GGCGTC G[GA]^CG[CT]C
AflII 951 CTTAAG C^TTAAG
AflIII 1342 ACATGT A^C[GA][CT]GT
AgeI 174 ACCGGT A^CCGGT
Alw44I 1746 GTGCAC G^TGCAC
ApaLI 1746 GTGCAC G^TGCAC
AvaII 468 GGACC G^G[AT]CC
BbvCI 1378 GCTGAGG CCTCAGC(-5/-2)
BcgI 743 GCAAAACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 306 CCGGG CC^[GC]GG
BfrI 951 CTTAAG C^TTAAG
BglII 2426 AGATCT A^GATCT
BpmI 356 CTCCAG CTGGAG(16/14)
BsaHI 1656 GGCGTC G[GA]^CG[CT]C
BsaI 1593 GAGACC GGTCTC(1/5)
BsaWI 174 ACCGGT [AT]^CCGG[AT]
BsgI 2269 GTGCAG GTGCAG(16/14)
BspDI 1704 ATCGAT AT^CGAT
BspHI 1387 TCATGA T^CATGA
BspLU11I 1342 ACATGT A^CATGT
BspMI 1773 ACCTGC ACCTGC(4/8)
BsrDI 1373 GCAATG GCAATG(2/0)
BsrFI 174 ACCGGT [GA]^CCGG[CT]
BstAPI 1459 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1251 GGTGACC G^GT[ACGT]ACC
BstPI 1251 GGTGACC G^GT[ACGT]ACC
BstXI 458 CCAAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2119 GCGC GCG^C
Cfr10I 174 ACCGGT [GA]^CCGG[CT]
ClaI 1704 ATCGAT AT^CGAT
DraI 795 TTTAAA TTT^AAA
DraII 343 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 209 TGGCCG [CT]^GGCC[GA]
EarI 400 GAAGAG CTCTTC(1/4)
EcoNI 947 CCTTCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 343 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1251 GGTGACC G^GT[ACGT]ACC
FauI 1711 CCCGC CCCGC(4/6)
HhaI 2119 GCGC GCG^C
Hin1I 1656 GGCGTC G[GA]^CG[CT]C
HinP1I 2119 GCGC G^CGC
Ksp632I 400 GAAGAG CTCTTC(1/4)
MspA1I 613 CAGCTG C[AC]G^C[GT]G
NciI 306 CCGGG CC^[GC]GG
NcoI 1331 CCATGG C^CATGG
PaeR7I 1306 CTCGAG C^TCGAG
PflMI 129 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 174 ACCGGT A^CCGGT
PshAI 1259 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 613 CAGCTG CAG^CTG
RcaI 1387 TCATGA T^CATGA
SalI 1164 GTCGAC G^TCGAC
SgrAI 173 CACCGGTG C[GA]^CCGG[CT]G
SpeI 421 ACTAGT A^CTAGT
SspI 2279 AATATT AAT^ATT
Van91I 129 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1306 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1164 GTCGAC GT^[AC][GT]AC
1299 GTAGAC
AciI 498 CCGC CCGC(-3/-1)
1712 CCGC
AspHI 1282 GTGCTC G[AT]GC[AT]^C
1746 GTGCAC
AvaI 194 CTCGGG C^[CT]CG[GA]G
1306 CTCGAG
BanII 100 GAGCCC G[GA]GC[CT]^C
2222 GGGCTC
BsiHKAI 1282 GTGCTC G[AT]GC[AT]^C
1746 GTGCAC
BsoBI 194 CTCGGG C^[CT]CG[GA]G
1306 CTCGAG
Bsp1407I 813 TGTACA T^GTACA
1413 TGTACA
BsrGI 813 TGTACA T^GTACA
1413 TGTACA
BssSI 203 CACGAG CACGAG(-5/-1)
2225 CTCGTG
Cfr13I 344 GGGCC G^G[ACGT]CC
468 GGACC
DsaI 1331 CCATGG C^C[GA][CT]GG
1778 CCGTGG
EcoRI 1218 GAATTC G^AATTC
2332 GAATTC
HapII 175 CCGG C^CGG
306 CCGG
HincII 1164 GTCGAC GT[CT]^[GA]AC
2026 GTCAAC
HindII 1164 GTCGAC GT[CT]^[GA]AC
2026 GTCAAC
HpaII 175 CCGG C^CGG
306 CCGG
MaeII 1087 ACGT A^CGT
1756 ACGT
MslI 685 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2212 CACACAAGTG
MspI 175 CCGG C^CGG
306 CCGG
NheI 1239 GCTAGC G^CTAGC
2201 GCTAGC
Sau96I 344 GGGCC G^G[ACGT]CC
468 GGACC
SphI 258 GCATGC GCATG^C
2230 GCATGC
SspBI 813 TGTACA T^GTACA
1413 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HgaI HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII