################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BciVI BclI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI BspEI BsrBI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PflFI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SmaI SnaBI Sse8387I StuI SwaI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcyI 1656 GGCGTC G[GA]^CG[CT]C AflII 951 CTTAAG C^TTAAG AflIII 1342 ACATGT A^C[GA][CT]GT AgeI 174 ACCGGT A^CCGGT Alw44I 1746 GTGCAC G^TGCAC ApaLI 1746 GTGCAC G^TGCAC AvaII 468 GGACC G^G[AT]CC BbvCI 1378 GCTGAGG CCTCAGC(-5/-2) BcgI 743 GCAAAACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 306 CCGGG CC^[GC]GG BfrI 951 CTTAAG C^TTAAG BglII 2426 AGATCT A^GATCT BpmI 356 CTCCAG CTGGAG(16/14) BsaHI 1656 GGCGTC G[GA]^CG[CT]C BsaI 1593 GAGACC GGTCTC(1/5) BsaWI 174 ACCGGT [AT]^CCGG[AT] BsgI 2269 GTGCAG GTGCAG(16/14) BspDI 1704 ATCGAT AT^CGAT BspHI 1387 TCATGA T^CATGA BspLU11I 1342 ACATGT A^CATGT BspMI 1773 ACCTGC ACCTGC(4/8) BsrDI 1373 GCAATG GCAATG(2/0) BsrFI 174 ACCGGT [GA]^CCGG[CT] BstAPI 1459 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1251 GGTGACC G^GT[ACGT]ACC BstPI 1251 GGTGACC G^GT[ACGT]ACC BstXI 458 CCAAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 2119 GCGC GCG^C Cfr10I 174 ACCGGT [GA]^CCGG[CT] ClaI 1704 ATCGAT AT^CGAT DraI 795 TTTAAA TTT^AAA DraII 343 GGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 209 TGGCCG [CT]^GGCC[GA] EarI 400 GAAGAG CTCTTC(1/4) EcoNI 947 CCTTCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 343 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1251 GGTGACC G^GT[ACGT]ACC FauI 1711 CCCGC CCCGC(4/6) HhaI 2119 GCGC GCG^C Hin1I 1656 GGCGTC G[GA]^CG[CT]C HinP1I 2119 GCGC G^CGC Ksp632I 400 GAAGAG CTCTTC(1/4) MspA1I 613 CAGCTG C[AC]G^C[GT]G NciI 306 CCGGG CC^[GC]GG NcoI 1331 CCATGG C^CATGG PaeR7I 1306 CTCGAG C^TCGAG PflMI 129 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 174 ACCGGT A^CCGGT PshAI 1259 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 613 CAGCTG CAG^CTG RcaI 1387 TCATGA T^CATGA SalI 1164 GTCGAC G^TCGAC SgrAI 173 CACCGGTG C[GA]^CCGG[CT]G SpeI 421 ACTAGT A^CTAGT SspI 2279 AATATT AAT^ATT Van91I 129 CCAGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1306 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 1164 GTCGAC GT^[AC][GT]AC 1299 GTAGAC AciI 498 CCGC CCGC(-3/-1) 1712 CCGC AspHI 1282 GTGCTC G[AT]GC[AT]^C 1746 GTGCAC AvaI 194 CTCGGG C^[CT]CG[GA]G 1306 CTCGAG BanII 100 GAGCCC G[GA]GC[CT]^C 2222 GGGCTC BsiHKAI 1282 GTGCTC G[AT]GC[AT]^C 1746 GTGCAC BsoBI 194 CTCGGG C^[CT]CG[GA]G 1306 CTCGAG Bsp1407I 813 TGTACA T^GTACA 1413 TGTACA BsrGI 813 TGTACA T^GTACA 1413 TGTACA BssSI 203 CACGAG CACGAG(-5/-1) 2225 CTCGTG Cfr13I 344 GGGCC G^G[ACGT]CC 468 GGACC DsaI 1331 CCATGG C^C[GA][CT]GG 1778 CCGTGG EcoRI 1218 GAATTC G^AATTC 2332 GAATTC HapII 175 CCGG C^CGG 306 CCGG HincII 1164 GTCGAC GT[CT]^[GA]AC 2026 GTCAAC HindII 1164 GTCGAC GT[CT]^[GA]AC 2026 GTCAAC HpaII 175 CCGG C^CGG 306 CCGG MaeII 1087 ACGT A^CGT 1756 ACGT MslI 685 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2212 CACACAAGTG MspI 175 CCGG C^CGG 306 CCGG NheI 1239 GCTAGC G^CTAGC 2201 GCTAGC Sau96I 344 GGGCC G^G[ACGT]CC 468 GGACC SphI 258 GCATGC GCATG^C 2230 GCATGC SspBI 813 TGTACA T^GTACA 1413 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BbvI BfaI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HgaI HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII