##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI AlwNI ApaI
AscI Asp700I Asp718I AspEI AviII
BalI BanI BbeI BbvCI BfrI
BglI BglII BsaBI BsaHI BsaI
BsgI BsiWI BsmBI Bsp1407I BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
CpoI DraIII Eam1105I EarI EcoNI
EcoO65I EcoRV FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MamI MluI
MluNI MscI NaeI NarI NdeI
NgoMIV NheI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI SspBI SwaI
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 555 TCCGGA T^CCGGA
AfaI 1850 GTAC GT^AC
Alw44I 2638 GTGCAC G^TGCAC
AocI 685 CCTGAGG CC^T[ACGT]AGG
Aor51HI 10 AGCGCT AGC^GCT
ApaLI 2638 GTGCAC G^TGCAC
AseI 2660 ATTAAT AT^TAAT
AsnI 2660 ATTAAT AT^TAAT
AspI 1037 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 148 CCCGAG C^[CT]CG[GA]G
AvrII 2546 CCTAGG C^CTAGG
BbrPI 1010 CACGTG CAC^GTG
BcgI 1669 GCAGGGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2687 GGATAC GTATCC(6/5)
BclI 1930 TGATCA T^GATCA
BlnI 2546 CCTAGG C^CTAGG
BlpI 115 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 115 GCTGAGC GC^T[ACGT]AGC
BsaWI 555 TCCGGA [AT]^CCGG[AT]
BseAI 555 TCCGGA T^CCGGA
BsmI 2510 GCATTC GAATGC(1/-1)
BsoBI 148 CCCGAG C^[CT]CG[GA]G
BspEI 555 TCCGGA T^CCGGA
BspMI 2608 ACCTGC ACCTGC(4/8)
BsrBI 1344 GAGCGG CCGCTC(-3/-3)
BsrFI 111 ACCGGC [GA]^CCGG[CT]
Bsu36I 685 CCTGAGG CC^T[ACGT]AGG
CelII 115 GCTGAGC GC^T[ACGT]AGC
Cfr10I 111 ACCGGC [GA]^CCGG[CT]
DraII 1476 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 952 GACAAATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 10 AGCGCT AGC^GCT
Eco81I 685 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1476 AGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 1930 TGATCA T^GATCA
MfeI 875 CAATTG C^AATTG
MroI 555 TCCGGA T^CCGGA
MunI 875 CAATTG C^AATTG
NcoI 1481 CCATGG C^CATGG
NotI 79 GCGGCCGC GC^GGCCGC
PflFI 1037 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1420 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1010 CACGTG CAC^GTG
PmlI 1010 CACGTG CAC^GTG
PpuMI 1476 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2660 ATTAAT AT^TAAT
PvuII 1072 CAGCTG CAG^CTG
RsaI 1850 GTAC GT^AC
SbfI 1636 CCTGCAGG CCTGCA^GG
SmlI 1899 CTCAAG C^T[CT][GA]AG
SpeI 1255 ACTAGT A^CTAGT
Sse8387I 1636 CCTGCAGG CCTGCA^GG
SspI 541 AATATT AAT^ATT
Tth111I 1037 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1420 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BamHI 552 GGATCC G^GATCC
2572 GGATCC
BsaAI 412 TACGTG [CT]AC^GT[GA]
1010 CACGTG
BsiEI 30 CGGCCG CG[GA][CT]^CG
80 CGGCCG
BspDI 1450 ATCGAT AT^CGAT
1677 ATCGAT
BspHI 966 TCATGA T^CATGA
1645 TCATGA
ClaI 1450 ATCGAT AT^CGAT
1677 ATCGAT
EagI 30 CGGCCG C^GGCCG
80 CGGCCG
EclXI 30 CGGCCG C^GGCCG
80 CGGCCG
Eco52I 30 CGGCCG C^GGCCG
80 CGGCCG
EcoRI 288 GAATTC G^AATTC
466 GAATTC
EcoT22I 934 ATGCAT ATGCA^T
2508 ATGCAT
HaeII 10 AGCGCT [GA]GCGC^[CT]
169 AGCGCC
NsiI 934 ATGCAT ATGCA^T
2508 ATGCAT
RcaI 966 TCATGA T^CATGA
1645 TCATGA
SacI 418 GAGCTC GAGCT^C
1395 GAGCTC
StuI 532 AGGCCT AGG^CCT
1633 AGGCCT
TfiI 1315 GATTC G^A[AT]TC
1611 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AccII AciI AcsI AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII