##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI AspEI AspI AvaI BalI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI HaeII Hin1I
HincII HindII HpaI KasI Ksp632I
KspI MfeI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 344 GGTACC G^GTACC
AflII 1677 CTTAAG C^TTAAG
AflIII 366 ACACGT A^C[GA][CT]GT
ApaI 353 GGGCCC GGGCC^C
Asp700I 456 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 344 GGTACC G^GTACC
AspHI 177 GAGCAC G[AT]GC[AT]^C
AvrII 1413 CCTAGG C^CTAGG
BanII 353 GGGCCC G[GA]GC[CT]^C
BbsI 272 GTCTTC GAAGAC(2/6)
BcnI 65 CCGGG CC^[GC]GG
BfrI 1677 CTTAAG C^TTAAG
BlnI 1413 CCTAGG C^CTAGG
BpuAI 272 GTCTTC GAAGAC(2/6)
BseRI 423 GAGGAG GAGGAG(10/8)
BsiHKAI 177 GAGCAC G[AT]GC[AT]^C
BspMI 764 ACCTGC ACCTGC(4/8)
BssHII 60 GCGCGC G^CGCGC
BstAPI 670 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 1236 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
FseI 239 GGCCGGCC GGCCGG^CC
HgaI 786 GCGTC GACGC(5/10)
HindIII 1657 AAGCTT A^AGCTT
KpnI 344 GGTACC GGTAC^C
MslI 1322 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 65 CCGGG CC^[GC]GG
PpuMI 1648 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 677 CTGCAG CTGCA^G
TfiI 1018 GATTC G^A[AT]TC
XmnI 456 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 149 GTAC GT^AC
345 GTAC
AviII 250 TGCGCA TGC^GCA
529 TGCGCA
BamHI 1069 GGATCC G^GATCC
1122 GGATCC
BanI 344 GGTACC G^G[CT][GA]CC
697 GGCACC
BsaBI 404 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1277 GATGAGCATC
BsgI 1574 CTGCAC GTGCAG(16/14)
510 GTGCAG
BsiEI 142 CGGCCG CG[GA][CT]^CG
238 CGGCCG
BsmAI 488 GAGAC GTCTC(1/5)
788 GTCTC
BsmBI 787 CGTCTC CGTCTC(1/5)
488 GAGACG
BsmFI 163 GGGAC GGGAC(10/14)
87 GTCCC
BsrFI 50 GCCGGC [GA]^CCGG[CT]
240 GCCGGC
Cfr10I 50 GCCGGC [GA]^CCGG[CT]
240 GCCGGC
DsaI 1306 CCATGG C^C[GA][CT]GG
1321 CCATGG
EaeI 142 CGGCCG [CT]^GGCC[GA]
238 CGGCCG
EagI 142 CGGCCG C^GGCCG
238 CGGCCG
EclXI 142 CGGCCG C^GGCCG
238 CGGCCG
Eco52I 142 CGGCCG C^GGCCG
238 CGGCCG
EcoNI 728 CCTTTCGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1603 CCTGGCAGAGG
FspI 250 TGCGCA TGC^GCA
529 TGCGCA
HphI 774 TCACC GGTGA(8/7)
1541 TCACC
MaeIII 370 GTCAC ^GT[ACGT]AC
1486 GTCAC
MamI 404 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1277 GATGAGCATC
NaeI 50 GCCGGC GCC^GGC
240 GCCGGC
NcoI 1306 CCATGG C^CATGG
1321 CCATGG
NgoMIV 50 GCCGGC G^CCGGC
240 GCCGGC
PvuII 339 CAGCTG CAG^CTG
863 CAGCTG
RsaI 149 GTAC GT^AC
345 GTAC
TaqI 205 TCGA T^CGA
854 TCGA
Tsp45I 370 GTCAC ^GT[GC]AC
1486 GTCAC
TthHB8I 205 TCGA T^CGA
854 TCGA
XcmI 820 CCAGCCTCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1219 CCATCTACTTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI Tru9I TseI TspRI
XhoII