##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BanII BbrPI BbvCI
BcgI BciVI BclI BglI BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BstEII BstPI
BstXI Bsu36I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT14I FauI FbaI FseI
FspI HincII HindII HpaI KpnI
KspI MamI MluI MroI NcoI
NheI NotI NruI PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI ScaI
SexAI SfiI SgrAI SmaI SpeI
SspBI StuI StyI SwaI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 497 CTTAAG C^TTAAG
Aor51HI 2455 AGCGCT AGC^GCT
AspHI 1803 GAGCAC G[AT]GC[AT]^C
BalI 2122 TGGCCA TGG^CCA
BanI 2058 GGCGCC G^G[CT][GA]CC
BbeI 2058 GGCGCC GGCGC^C
BfrI 497 CTTAAG C^TTAAG
BmyI 1803 GAGCAC G[AGT]GC[ACT]^C
BsaAI 1558 TACGTA [CT]AC^GT[GA]
BsgI 502 GTGCAG GTGCAG(16/14)
BsiHKAI 1803 GAGCAC G[AT]GC[AT]^C
BsmAI 1826 GAGAC GTCTC(1/5)
BsmI 718 GAATGC GAATGC(1/-1)
Bsp1286I 1803 GAGCAC G[AGT]GC[ACT]^C
BsrBI 2025 GAGCGG CCGCTC(-3/-3)
BsrDI 1183 CATTGC GCAATG(2/0)
BsrFI 116 GCCGGC [GA]^CCGG[CT]
BssHII 152 GCGCGC G^CGCGC
BssSI 1995 CACGAG CACGAG(-5/-1)
Bst1107I 674 GTATAC GTA^TAC
BstBI 604 TTCGAA TT^CGAA
BstZ17I 674 GTATAC GTA^TAC
Cfr10I 116 GCCGGC [GA]^CCGG[CT]
DraI 2070 TTTAAA TTT^AAA
Eco47III 2455 AGCGCT AGC^GCT
EcoRI 1302 GAATTC G^AATTC
EcoRV 639 GATATC GAT^ATC
HindIII 652 AAGCTT A^AGCTT
HphI 91 GGTGA GGTGA(8/7)
KasI 2058 GGCGCC G^GCGCC
MfeI 1834 CAATTG C^AATTG
MluNI 2122 TGGCCA TGG^CCA
MscI 2122 TGGCCA TGG^CCA
MspA1I 2502 CAGCGG C[AC]G^C[GT]G
MunI 1834 CAATTG C^AATTG
NaeI 116 GCCGGC GCC^GGC
NarI 2058 GGCGCC GG^CGCC
NdeI 341 CATATG CA^TATG
NgoMIV 116 GCCGGC G^CCGGC
NspI 1651 GCATGC [GA]CATG^[CT]
NspV 604 TTCGAA TT^CGAA
PflMI 437 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SbfI 1550 CCTGCAGG CCTGCA^GG
SfoI 2058 GGCGCC GGC^GCC
SfuI 604 TTCGAA TT^CGAA
SnaBI 1558 TACGTA TAC^GTA
SphI 1651 GCATGC GCATG^C
Sse8387I 1550 CCTGCAGG CCTGCA^GG
Van91I 437 CCAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 415 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1538 CAGGCACTG
AseI 765 ATTAAT AT^TAAT
1224 ATTAAT
AsnI 765 ATTAAT AT^TAAT
1224 ATTAAT
AvaI 1615 CTCGAG C^[CT]CG[GA]G
1893 CTCGAG
BamHI 1813 GGATCC G^GATCC
2105 GGATCC
BglII 461 AGATCT A^GATCT
2384 AGATCT
BlpI 2214 GCTAAGC GC^T[ACGT]AGC
2458 GCTTAGC
Bpu1102I 2214 GCTAAGC GC^T[ACGT]AGC
2458 GCTTAGC
BseRI 2558 GAGGAG GAGGAG(10/8)
2568 GAGGAG
BsmFI 434 GTCCC GGGAC(10/14)
1263 GTCCC
BsoBI 1615 CTCGAG C^[CT]CG[GA]G
1893 CTCGAG
BstAPI 1646 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1770 GCAGTTAATGC
CelII 2214 GCTAAGC GC^T[ACGT]AGC
2458 GCTTAGC
EaeI 1684 CGGCCA [CT]^GGCC[GA]
2122 TGGCCA
MslI 1682 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2312 CACTACAATG
PaeR7I 1615 CTCGAG C^TCGAG
1893 CTCGAG
PshBI 765 ATTAAT AT^TAAT
1224 ATTAAT
PstI 1551 CTGCAG CTGCA^G
1644 CTGCAG
SapI 2451 GAAGAGC GCTCTTC(1/4)
2550 GAAGAGC
SspI 471 AATATT AAT^ATT
1869 AATATT
XbaI 615 TCTAGA T^CTAGA
1020 TCTAGA
XhoI 1615 CTCGAG C^TCGAG
1893 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI Asp700I
AvaII BbsI BbvI BcnI BfaI
BpmI BpuAI BsaHI BsaJI BsiYI
BslI BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
EcoT22I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI