##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI Asp700I
AspEI AspI BamHI BbeI BbvCI
BciVI BfrI BglII BsaBI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacII SbfI ScaI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 974 GGTACC G^GTACC
AccI 177 GTCGAC GT^[AC][GT]AC
AcsI 653 AAATTC [GA]^AATT[CT]
AcyI 2134 GACGCC G[GA]^CG[CT]C
AflIII 1983 ACACGT A^C[GA][CT]GT
ApoI 653 AAATTC [GA]^AATT[CT]
AseI 1586 ATTAAT AT^TAAT
AsnI 1586 ATTAAT AT^TAAT
Asp718I 974 GGTACC G^GTACC
AvaI 723 CCCGGG C^[CT]CG[GA]G
AvrII 2196 CCTAGG C^CTAGG
BalI 1508 TGGCCA TGG^CCA
BanI 974 GGTACC G^G[CT][GA]CC
BbrPI 1289 CACGTG CAC^GTG
BbsI 409 GAAGAC GAAGAC(2/6)
BclI 1800 TGATCA T^GATCA
BlnI 2196 CCTAGG C^CTAGG
BpuAI 409 GAAGAC GAAGAC(2/6)
BsaHI 2134 GACGCC G[GA]^CG[CT]C
BsiEI 1471 CGACCG CG[GA][CT]^CG
BsmBI 2118 CGTCTC CGTCTC(1/5)
BsoBI 723 CCCGGG C^[CT]CG[GA]G
BspMI 104 GCAGGT ACCTGC(4/8)
BsrDI 1636 CATTGC GCAATG(2/0)
BssHII 2012 GCGCGC G^CGCGC
BssSI 414 CTCGTG CACGAG(-5/-1)
BstYI 273 AGATCC [GA]^GATC[CT]
DraIII 474 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 563 GAAGAG CTCTTC(1/4)
FbaI 1800 TGATCA T^GATCA
Hin1I 2134 GACGCC G[GA]^CG[CT]C
KpnI 974 GGTACC GGTAC^C
Ksp632I 563 GAAGAG CTCTTC(1/4)
MflI 273 AGATCC [GA]^GATC[CT]
MluNI 1508 TGGCCA TGG^CCA
MscI 1508 TGGCCA TGG^CCA
MslI 514 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1278 ACATGC [GA]CATG^[CT]
PmaCI 1289 CACGTG CAC^GTG
PmlI 1289 CACGTG CAC^GTG
PshAI 2194 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1586 ATTAAT AT^TAAT
PvuII 1676 CAGCTG CAG^CTG
SacI 1000 GAGCTC GAGCT^C
SalI 177 GTCGAC G^TCGAC
SapI 563 GAAGAGC GCTCTTC(1/4)
SexAI 1989 ACCTGGT A^CC[AT]GGT
SmaI 723 CCCGGG CCC^GGG
SspI 1538 AATATT AAT^ATT
XhoII 273 AGATCC [GA]^GATC[CT]
XmaCI 723 CCCGGG C^CCGGG
XmaI 723 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 1143 GTGCAC G^TGCAC
1994 GTGCAC
AlwI 274 GATCC GGATC(4/5)
1781 GATCC
ApaI 1652 GGGCCC GGGCC^C
2228 GGGCCC
ApaLI 1143 GTGCAC G^TGCAC
1994 GTGCAC
AviII 61 TGCGCA TGC^GCA
576 TGCGCA
BcgI 2002 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1182 GCACTAACATCG
BfaI 2051 CTAG C^TAG
2197 CTAG
BglI 1287 GCCACGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2031 GCCTACAGGGC
BlpI 371 GCTCAGC GC^T[ACGT]AGC
1282 GCTCAGC
Bpu1102I 371 GCTCAGC GC^T[ACGT]AGC
1282 GCTCAGC
BsaAI 1289 CACGTG [CT]AC^GT[GA]
1984 CACGTA
BsaWI 468 TCCGGT [AT]^CCGG[AT]
1147 ACCGGA
BsmI 634 GAATGC GAATGC(1/-1)
1925 GAATGC
Bsp1407I 806 TGTACA T^GTACA
1600 TGTACA
BsrGI 806 TGTACA T^GTACA
1600 TGTACA
BstAPI 362 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1920 GCAACGAATGC
BstXI 885 CCAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2241 CCACCCTATTGG
CelII 371 GCTCAGC GC^T[ACGT]AGC
1282 GCTCAGC
EaeI 1508 TGGCCA [CT]^GGCC[GA]
2211 TGGCCG
FspI 61 TGCGCA TGC^GCA
576 TGCGCA
HaeII 1460 AGCGCC [GA]GCGC^[CT]
1563 AGCGCC
HincII 177 GTCGAC GT[CT]^[GA]AC
1391 GTCAAC
HindII 177 GTCGAC GT[CT]^[GA]AC
1391 GTCAAC
MaeI 2051 CTAG C^TAG
2197 CTAG
PflMI 110 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1206 CCAAGAGGTGG
PstI 1067 CTGCAG CTGCA^G
1817 CTGCAG
SspBI 806 TGTACA T^GTACA
1600 TGTACA
TfiI 1100 GAATC G^A[AT]TC
1738 GATTC
Van91I 110 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1206 CCAAGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI