################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI Asp700I AspEI AspI BamHI BbeI BbvCI BciVI BfrI BglII BsaBI BseAI BsiWI BspDI BspEI BspHI BspLU11I Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RcaI RsrII SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 974 GGTACC G^GTACC AccI 177 GTCGAC GT^[AC][GT]AC AcsI 653 AAATTC [GA]^AATT[CT] AcyI 2134 GACGCC G[GA]^CG[CT]C AflIII 1983 ACACGT A^C[GA][CT]GT ApoI 653 AAATTC [GA]^AATT[CT] AseI 1586 ATTAAT AT^TAAT AsnI 1586 ATTAAT AT^TAAT Asp718I 974 GGTACC G^GTACC AvaI 723 CCCGGG C^[CT]CG[GA]G AvrII 2196 CCTAGG C^CTAGG BalI 1508 TGGCCA TGG^CCA BanI 974 GGTACC G^G[CT][GA]CC BbrPI 1289 CACGTG CAC^GTG BbsI 409 GAAGAC GAAGAC(2/6) BclI 1800 TGATCA T^GATCA BlnI 2196 CCTAGG C^CTAGG BpuAI 409 GAAGAC GAAGAC(2/6) BsaHI 2134 GACGCC G[GA]^CG[CT]C BsiEI 1471 CGACCG CG[GA][CT]^CG BsmBI 2118 CGTCTC CGTCTC(1/5) BsoBI 723 CCCGGG C^[CT]CG[GA]G BspMI 104 GCAGGT ACCTGC(4/8) BsrDI 1636 CATTGC GCAATG(2/0) BssHII 2012 GCGCGC G^CGCGC BssSI 414 CTCGTG CACGAG(-5/-1) BstYI 273 AGATCC [GA]^GATC[CT] DraIII 474 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 563 GAAGAG CTCTTC(1/4) FbaI 1800 TGATCA T^GATCA Hin1I 2134 GACGCC G[GA]^CG[CT]C KpnI 974 GGTACC GGTAC^C Ksp632I 563 GAAGAG CTCTTC(1/4) MflI 273 AGATCC [GA]^GATC[CT] MluNI 1508 TGGCCA TGG^CCA MscI 1508 TGGCCA TGG^CCA MslI 514 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 1278 ACATGC [GA]CATG^[CT] PmaCI 1289 CACGTG CAC^GTG PmlI 1289 CACGTG CAC^GTG PshAI 2194 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1586 ATTAAT AT^TAAT PvuII 1676 CAGCTG CAG^CTG SacI 1000 GAGCTC GAGCT^C SalI 177 GTCGAC G^TCGAC SapI 563 GAAGAGC GCTCTTC(1/4) SexAI 1989 ACCTGGT A^CC[AT]GGT SmaI 723 CCCGGG CCC^GGG SspI 1538 AATATT AAT^ATT XhoII 273 AGATCC [GA]^GATC[CT] XmaCI 723 CCCGGG C^CCGGG XmaI 723 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Alw44I 1143 GTGCAC G^TGCAC 1994 GTGCAC AlwI 274 GATCC GGATC(4/5) 1781 GATCC ApaI 1652 GGGCCC GGGCC^C 2228 GGGCCC ApaLI 1143 GTGCAC G^TGCAC 1994 GTGCAC AviII 61 TGCGCA TGC^GCA 576 TGCGCA BcgI 2002 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1182 GCACTAACATCG BfaI 2051 CTAG C^TAG 2197 CTAG BglI 1287 GCCACGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2031 GCCTACAGGGC BlpI 371 GCTCAGC GC^T[ACGT]AGC 1282 GCTCAGC Bpu1102I 371 GCTCAGC GC^T[ACGT]AGC 1282 GCTCAGC BsaAI 1289 CACGTG [CT]AC^GT[GA] 1984 CACGTA BsaWI 468 TCCGGT [AT]^CCGG[AT] 1147 ACCGGA BsmI 634 GAATGC GAATGC(1/-1) 1925 GAATGC Bsp1407I 806 TGTACA T^GTACA 1600 TGTACA BsrGI 806 TGTACA T^GTACA 1600 TGTACA BstAPI 362 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1920 GCAACGAATGC BstXI 885 CCAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2241 CCACCCTATTGG CelII 371 GCTCAGC GC^T[ACGT]AGC 1282 GCTCAGC EaeI 1508 TGGCCA [CT]^GGCC[GA] 2211 TGGCCG FspI 61 TGCGCA TGC^GCA 576 TGCGCA HaeII 1460 AGCGCC [GA]GCGC^[CT] 1563 AGCGCC HincII 177 GTCGAC GT[CT]^[GA]AC 1391 GTCAAC HindII 177 GTCGAC GT[CT]^[GA]AC 1391 GTCAAC MaeI 2051 CTAG C^TAG 2197 CTAG PflMI 110 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1206 CCAAGAGGTGG PstI 1067 CTGCAG CTGCA^G 1817 CTGCAG SspBI 806 TGTACA T^GTACA 1600 TGTACA TfiI 1100 GAATC G^A[AT]TC 1738 GATTC Van91I 110 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1206 CCAAGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI