##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BbeI BbrPI
BbsI BciVI BclI BfrI BlnI
BpuAI BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FauI FbaI
FseI HindIII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 419 GACGTC GACGT^C
AccII 1000 CGCG CG^CG
AcsI 348 GAATTC [GA]^AATT[CT]
AcyI 419 GACGTC G[GA]^CG[CT]C
Aor51HI 1160 AGCGCT AGC^GCT
ApoI 348 GAATTC [GA]^AATT[CT]
AvaI 1084 CCCGAG C^[CT]CG[GA]G
BamHI 1261 GGATCC G^GATCC
BcgI 1453 CGACCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1435 GCCAAGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1281 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1281 GCTGAGC GC^T[ACGT]AGC
BsaBI 386 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 419 GACGTC G[GA]^CG[CT]C
BsaI 1838 GGTCTC GGTCTC(1/5)
BsoBI 1084 CCCGAG C^[CT]CG[GA]G
BspMI 213 ACCTGC ACCTGC(4/8)
BsrBI 1087 GAGCGG CCGCTC(-3/-3)
BssSI 254 CTCGTG CACGAG(-5/-1)
BstEII 187 GGTGACC G^GT[ACGT]ACC
BstPI 187 GGTGACC G^GT[ACGT]ACC
BstUI 1000 CGCG CG^CG
CelII 1281 GCTGAGC GC^T[ACGT]AGC
DraI 1816 TTTAAA TTT^AAA
DraIII 647 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 177 CCACGG C^C[GA][CT]GG
EaeI 433 TGGCCG [CT]^GGCC[GA]
EarI 1660 CTCTTC CTCTTC(1/4)
Eco47III 1160 AGCGCT AGC^GCT
EcoO65I 187 GGTGACC G^GT[ACGT]ACC
EcoRI 348 GAATTC G^AATTC
HaeII 1160 AGCGCT [GA]GCGC^[CT]
HgaI 153 GCGTC GACGC(5/10)
Hin1I 419 GACGTC G[GA]^CG[CT]C
HincII 805 GTCGAC GT[CT]^[GA]AC
HindII 805 GTCGAC GT[CT]^[GA]AC
Ksp632I 1660 CTCTTC CTCTTC(1/4)
MaeII 420 ACGT A^CGT
MamI 386 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1000 CGCG CG^CG
NaeI 1027 GCCGGC GCC^GGC
NgoMIV 1027 GCCGGC G^CCGGC
PshAI 1270 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 305 GAGCTC GAGCT^C
SalI 805 GTCGAC G^TCGAC
TaqI 806 TCGA T^CGA
TthHB8I 806 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 805 GTCGAC GT^[AC][GT]AC
878 GTCTAC
AlwNI 311 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
846 CAGGCACTG
AocI 84 CCTCAGG CC^T[ACGT]AGG
192 CCTCAGG
ApaI 259 GGGCCC GGGCC^C
968 GGGCCC
AviII 1726 TGCGCA TGC^GCA
1751 TGCGCA
BanI 226 GGTGCC G^G[CT][GA]CC
793 GGCACC
BbvCI 633 CCTCAGC CCTCAGC(-5/-2)
658 CCTCAGC
BglII 1469 AGATCT A^GATCT
1884 AGATCT
BsrFI 563 ACCGGC [GA]^CCGG[CT]
1027 GCCGGC
BstXI 177 CCACGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1486 CCAGCTCAGTGG
Bsu36I 84 CCTCAGG CC^T[ACGT]AGG
192 CCTCAGG
Cfr10I 563 ACCGGC [GA]^CCGG[CT]
1027 GCCGGC
Eco81I 84 CCTCAGG CC^T[ACGT]AGG
192 CCTCAGG
FspI 1726 TGCGCA TGC^GCA
1751 TGCGCA
MseI 1675 TTAA T^TAA
1817 TTAA
PleI 443 GACTC GAGTC(4/5)
925 GACTC
PstI 317 CTGCAG CTGCA^G
981 CTGCAG
SmlI 219 CTCAAG C^T[CT][GA]AG
1134 CTCAAG
Tru9I 1675 TTAA T^TAA
1817 TTAA
Tsp509I 349 AATT ^AATT
1605 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I TspRI XcmI
XhoII