################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BbeI BbrPI BbsI BciVI BclI BfrI BlnI BpuAI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FauI FbaI FseI HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 419 GACGTC GACGT^C AccII 1000 CGCG CG^CG AcsI 348 GAATTC [GA]^AATT[CT] AcyI 419 GACGTC G[GA]^CG[CT]C Aor51HI 1160 AGCGCT AGC^GCT ApoI 348 GAATTC [GA]^AATT[CT] AvaI 1084 CCCGAG C^[CT]CG[GA]G BamHI 1261 GGATCC G^GATCC BcgI 1453 CGACCCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1435 GCCAAGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1281 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1281 GCTGAGC GC^T[ACGT]AGC BsaBI 386 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 419 GACGTC G[GA]^CG[CT]C BsaI 1838 GGTCTC GGTCTC(1/5) BsoBI 1084 CCCGAG C^[CT]CG[GA]G BspMI 213 ACCTGC ACCTGC(4/8) BsrBI 1087 GAGCGG CCGCTC(-3/-3) BssSI 254 CTCGTG CACGAG(-5/-1) BstEII 187 GGTGACC G^GT[ACGT]ACC BstPI 187 GGTGACC G^GT[ACGT]ACC BstUI 1000 CGCG CG^CG CelII 1281 GCTGAGC GC^T[ACGT]AGC DraI 1816 TTTAAA TTT^AAA DraIII 647 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 177 CCACGG C^C[GA][CT]GG EaeI 433 TGGCCG [CT]^GGCC[GA] EarI 1660 CTCTTC CTCTTC(1/4) Eco47III 1160 AGCGCT AGC^GCT EcoO65I 187 GGTGACC G^GT[ACGT]ACC EcoRI 348 GAATTC G^AATTC HaeII 1160 AGCGCT [GA]GCGC^[CT] HgaI 153 GCGTC GACGC(5/10) Hin1I 419 GACGTC G[GA]^CG[CT]C HincII 805 GTCGAC GT[CT]^[GA]AC HindII 805 GTCGAC GT[CT]^[GA]AC Ksp632I 1660 CTCTTC CTCTTC(1/4) MaeII 420 ACGT A^CGT MamI 386 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1000 CGCG CG^CG NaeI 1027 GCCGGC GCC^GGC NgoMIV 1027 GCCGGC G^CCGGC PshAI 1270 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 305 GAGCTC GAGCT^C SalI 805 GTCGAC G^TCGAC TaqI 806 TCGA T^CGA TthHB8I 806 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 805 GTCGAC GT^[AC][GT]AC 878 GTCTAC AlwNI 311 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 846 CAGGCACTG AocI 84 CCTCAGG CC^T[ACGT]AGG 192 CCTCAGG ApaI 259 GGGCCC GGGCC^C 968 GGGCCC AviII 1726 TGCGCA TGC^GCA 1751 TGCGCA BanI 226 GGTGCC G^G[CT][GA]CC 793 GGCACC BbvCI 633 CCTCAGC CCTCAGC(-5/-2) 658 CCTCAGC BglII 1469 AGATCT A^GATCT 1884 AGATCT BsrFI 563 ACCGGC [GA]^CCGG[CT] 1027 GCCGGC BstXI 177 CCACGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1486 CCAGCTCAGTGG Bsu36I 84 CCTCAGG CC^T[ACGT]AGG 192 CCTCAGG Cfr10I 563 ACCGGC [GA]^CCGG[CT] 1027 GCCGGC Eco81I 84 CCTCAGG CC^T[ACGT]AGG 192 CCTCAGG FspI 1726 TGCGCA TGC^GCA 1751 TGCGCA MseI 1675 TTAA T^TAA 1817 TTAA PleI 443 GACTC GAGTC(4/5) 925 GACTC PstI 317 CTGCAG CTGCA^G 981 CTGCAG SmlI 219 CTCAAG C^T[CT][GA]AG 1134 CTCAAG Tru9I 1675 TTAA T^TAA 1817 TTAA Tsp509I 349 AATT ^AATT 1605 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI XcmI XhoII