##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BamHI BanII
BbeI BcgI BciVI BclI BfrI
BglI BlnI BsaBI BsaWI BseAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BsrBI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SapI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SspI
SwaI TfiI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 303 GACGTC GACGT^C
AccI 1940 GTCGAC GT^[AC][GT]AC
AccII 477 CGCG CG^CG
AflIII 1944 ACATGT A^C[GA][CT]GT
Alw44I 1447 GTGCAC G^TGCAC
AocI 326 CCTCAGG CC^T[ACGT]AGG
ApaLI 1447 GTGCAC G^TGCAC
Asp700I 1035 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2195 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1724 TGGCCA TGG^CCA
BbrPI 1444 CACGTG CAC^GTG
BcnI 49 CCGGG CC^[GC]GG
BglII 659 AGATCT A^GATCT
BlpI 2216 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2216 GCTCAGC GC^T[ACGT]AGC
BsaAI 1444 CACGTG [CT]AC^GT[GA]
BsaI 45 GAGACC GGTCTC(1/5)
BsiEI 991 CGGTCG CG[GA][CT]^CG
Bsp1407I 638 TGTACA T^GTACA
BspLU11I 1944 ACATGT A^CATGT
BsrGI 638 TGTACA T^GTACA
BstEII 852 GGTGACC G^GT[ACGT]ACC
BstPI 852 GGTGACC G^GT[ACGT]ACC
BstUI 477 CGCG CG^CG
Bsu36I 326 CCTCAGG CC^T[ACGT]AGG
CelII 2216 GCTCAGC GC^T[ACGT]AGC
Eco81I 326 CCTCAGG CC^T[ACGT]AGG
EcoNI 1656 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 852 GGTGACC G^GT[ACGT]ACC
MluNI 1724 TGGCCA TGG^CCA
MscI 1724 TGGCCA TGG^CCA
MseI 2179 TTAA T^TAA
MslI 642 CACACTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 477 CGCG CG^CG
NciI 49 CCGGG CC^[GC]GG
NheI 2067 GCTAGC G^CTAGC
PflFI 2195 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1444 CACGTG CAC^GTG
PmlI 1444 CACGTG CAC^GTG
SalI 1940 GTCGAC G^TCGAC
SbfI 1404 CCTGCAGG CCTGCA^GG
SpeI 645 ACTAGT A^CTAGT
SphI 1058 GCATGC GCATG^C
Sse8387I 1404 CCTGCAGG CCTGCA^GG
SspBI 638 TGTACA T^GTACA
StuI 533 AGGCCT AGG^CCT
Tru9I 2179 TTAA T^TAA
Tsp509I 1541 AATT ^AATT
Tth111I 2195 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1500 TCTAGA T^CTAGA
XmnI 1035 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BanI 875 GGCACC G^G[CT][GA]CC
996 GGTGCC
BspHI 761 TCATGA T^CATGA
893 TCATGA
BsrDI 1841 CATTGC GCAATG(2/0)
2053 GCAATG
BsrFI 986 GCCGGC [GA]^CCGG[CT]
1398 GCCGGC
BssSI 1450 CACGAG CACGAG(-5/-1)
616 CTCGTG
BstYI 659 AGATCT [GA]^GATC[CT]
1998 GGATCT
Cfr10I 986 GCCGGC [GA]^CCGG[CT]
1398 GCCGGC
DrdI 128 GACTTCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
297 GACTCTGACGTC
EarI 166 CTCTTC CTCTTC(1/4)
1336 GAAGAG
HincII 580 GTTGAC GT[CT]^[GA]AC
1940 GTCGAC
HindII 580 GTTGAC GT[CT]^[GA]AC
1940 GTCGAC
Ksp632I 166 CTCTTC CTCTTC(1/4)
1336 GAAGAG
MflI 659 AGATCT [GA]^GATC[CT]
1998 GGATCT
NaeI 986 GCCGGC GCC^GGC
1398 GCCGGC
NgoMIV 986 GCCGGC G^CCGGC
1398 GCCGGC
NspI 1058 GCATGC [GA]CATG^[CT]
1944 ACATGT
PpuMI 610 GGGACCC [GA]G^G[AT]CC[CT]
2124 GGGACCC
PvuII 430 CAGCTG CAG^CTG
1610 CAGCTG
RcaI 761 TCATGA T^CATGA
893 TCATGA
SexAI 1025 ACCTGGT A^CC[AT]GGT
1415 ACCTGGT
XhoII 659 AGATCT [GA]^GATC[CT]
1998 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaII BbsI BbvCI
BbvI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI