##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BcgI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 994 GGTACC G^GTACC
AccI 573 GTAGAC GT^[AC][GT]AC
AciI 1556 CCGC CCGC(-3/-1)
AcsI 285 AAATTT [GA]^AATT[CT]
AlwNI 1863 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1188 CCTAAGG CC^T[ACGT]AGG
ApaI 825 GGGCCC GGGCC^C
ApoI 285 AAATTT [GA]^AATT[CT]
Asp718I 994 GGTACC G^GTACC
BanI 994 GGTACC G^G[CT][GA]CC
BbrPI 684 CACGTG CAC^GTG
BbvCI 1649 CCTCAGC CCTCAGC(-5/-2)
BciVI 1298 GGATAC GTATCC(6/5)
BclI 1964 TGATCA T^GATCA
BcnI 912 CCGGG CC^[GC]GG
BlpI 436 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 436 GCTTAGC GC^T[ACGT]AGC
BsaAI 684 CACGTG [CT]AC^GT[GA]
BseRI 240 GAGGAG GAGGAG(10/8)
BsgI 384 GTGCAG GTGCAG(16/14)
Bsp1407I 1260 TGTACA T^GTACA
BsrGI 1260 TGTACA T^GTACA
BstXI 1403 CCATCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 369 AGATCC [GA]^GATC[CT]
Bsu36I 1188 CCTAAGG CC^T[ACGT]AGG
CelII 436 GCTTAGC GC^T[ACGT]AGC
DsaI 1676 CCATGG C^C[GA][CT]GG
Eco81I 1188 CCTAAGG CC^T[ACGT]AGG
EcoNI 132 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1676 CCATGG C^C[AT][AT]GG
FbaI 1964 TGATCA T^GATCA
HincII 837 GTTGAC GT[CT]^[GA]AC
HindII 837 GTTGAC GT[CT]^[GA]AC
HindIII 434 AAGCTT A^AGCTT
KpnI 994 GGTACC GGTAC^C
MflI 369 AGATCC [GA]^GATC[CT]
MspA1I 1849 CAGCTG C[AC]G^C[GT]G
NciI 912 CCGGG CC^[GC]GG
NcoI 1676 CCATGG C^CATGG
PflMI 816 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 684 CACGTG CAC^GTG
PmlI 684 CACGTG CAC^GTG
PpuMI 1749 GGGACCC [GA]G^G[AT]CC[CT]
PstI 654 CTGCAG CTGCA^G
PvuII 1849 CAGCTG CAG^CTG
SapI 974 GAAGAGC GCTCTTC(1/4)
ScaI 665 AGTACT AGT^ACT
SspBI 1260 TGTACA T^GTACA
SspI 84 AATATT AAT^ATT
StyI 1676 CCATGG C^C[AT][AT]GG
TaqI 614 TCGA T^CGA
TspRI 1801 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 614 TCGA T^CGA
Van91I 816 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 369 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanII 825 GGGCCC G[GA]GC[CT]^C
1386 GAGCCC
BsaWI 625 TCCGGT [AT]^CCGG[AT]
1295 ACCGGA
BsmFI 1749 GGGAC GGGAC(10/14)
446 GTCCC
BsmI 350 GAATGC GAATGC(1/-1)
554 GCATTC
BspLU11I 865 ACATGT A^CATGT
1363 ACATGT
BspMI 131 ACCTGC ACCTGC(4/8)
857 ACCTGC
Cac8I 411 GCCAGC GC[ACGT]^[ACGT]GC
1202 GCAGGC
DraII 1378 AGGGCCT [GA]G^G[ACGT]CC[CT]
1749 GGGACCC
EcoO109I 1378 AGGGCCT [GA]G^G[ACGT]CC[CT]
1749 GGGACCC
HgaI 1518 GACGC GACGC(5/10)
1880 GCGTC
MwoI 146 GCTGGATGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1928 GCTCTCATTGC
SfcI 571 CTGTAG C^T[GA][CT]AG
654 CTGCAG
StuI 705 AGGCCT AGG^CCT
1204 AGGCCT
TfiI 1182 GATTC G^A[AT]TC
1716 GAATC
XcmI 1614 CCACCCCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1619 CCAGAGGTGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AfaI AflIII AluI AlwI Asp700I
AvaII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SmlI Tru9I TseI Tsp45I Tsp509I
XmnI