################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## Acc65I AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BsaBI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstXI BstYI ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 862 GACGTC GACGT^C AccI 670 GTATAC GT^[AC][GT]AC AcyI 862 GACGTC G[GA]^CG[CT]C AocI 961 CCTCAGG CC^T[ACGT]AGG Aor51HI 851 AGCGCT AGC^GCT BalI 345 TGGCCA TGG^CCA BlpI 351 GCTGAGC GC^T[ACGT]AGC BmyI 564 GGGCAC G[AGT]GC[ACT]^C Bpu1102I 351 GCTGAGC GC^T[ACGT]AGC BsaAI 583 TACGTA [CT]AC^GT[GA] BsaHI 862 GACGTC G[GA]^CG[CT]C BseRI 324 CTCCTC GAGGAG(10/8) BsmI 179 GCATTC GAATGC(1/-1) Bsp1286I 564 GGGCAC G[AGT]GC[ACT]^C BsrDI 83 CATTGC GCAATG(2/0) Bst1107I 670 GTATAC GTA^TAC BstZ17I 670 GTATAC GTA^TAC Bsu36I 961 CCTCAGG CC^T[ACGT]AGG CelII 351 GCTGAGC GC^T[ACGT]AGC DraI 1043 TTTAAA TTT^AAA DraII 770 AGGACCT [GA]G^G[ACGT]CC[CT] Eco47III 851 AGCGCT AGC^GCT Eco81I 961 CCTCAGG CC^T[ACGT]AGG EcoNI 810 CCTGATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 770 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 553 GATATC GAT^ATC FauI 806 CCCGC CCCGC(4/6) HaeII 851 AGCGCT [GA]GCGC^[CT] Hin1I 862 GACGTC G[GA]^CG[CT]C HincII 1155 GTTGAC GT[CT]^[GA]AC HindII 1155 GTTGAC GT[CT]^[GA]AC MluNI 345 TGGCCA TGG^CCA MscI 345 TGGCCA TGG^CCA PaeR7I 164 CTCGAG C^TCGAG PpuMI 770 AGGACCT [GA]G^G[AT]CC[CT] PvuII 349 CAGCTG CAG^CTG SapI 876 GCTCTTC GCTCTTC(1/4) ScaI 299 AGTACT AGT^ACT SnaBI 583 TACGTA TAC^GTA SphI 356 GCATGC GCATG^C XhoI 164 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AvaI 164 CTCGAG C^[CT]CG[GA]G 832 CTCGGG AvaII 542 GGTCC G^G[AT]CC 771 GGACC BanI 565 GGCACC G^G[CT][GA]CC 1164 GGCACC BbsI 141 GAAGAC GAAGAC(2/6) 897 GTCTTC BcgI 152 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 504 GCAGCTTTATCG BfaI 329 CTAG C^TAG 1061 CTAG BpmI 547 CTCCAG CTGGAG(16/14) 453 CTGGAG BpuAI 141 GAAGAC GAAGAC(2/6) 897 GTCTTC BsaI 487 GGTCTC GGTCTC(1/5) 1298 GGTCTC BsgI 947 GTGCAG GTGCAG(16/14) 1119 GTGCAG BsoBI 164 CTCGAG C^[CT]CG[GA]G 832 CTCGGG BsrFI 707 GCCGGT [GA]^CCGG[CT] 887 GCCGGT BstAPI 356 GCATGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 994 GCAGCCGCTGC Cfr10I 707 GCCGGT [GA]^CCGG[CT] 887 GCCGGT DpnII 423 GATC ^GATC 646 GATC EaeI 345 TGGCCA [CT]^GGCC[GA] 381 TGGCCG EarI 877 CTCTTC CTCTTC(1/4) 625 GAAGAG HapII 708 CCGG C^CGG 888 CCGG HgaI 682 GACGC GACGC(5/10) 1158 GACGC HpaII 708 CCGG C^CGG 888 CCGG HphI 147 GGTGA GGTGA(8/7) 1307 TCACC Ksp632I 877 CTCTTC CTCTTC(1/4) 625 GAAGAG MaeI 329 CTAG C^TAG 1061 CTAG MaeII 584 ACGT A^CGT 863 ACGT MboI 423 GATC ^GATC 646 GATC MseI 401 TTAA T^TAA 1044 TTAA MslI 333 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1017 CATTTTGATG MspA1I 349 CAGCTG C[AC]G^C[GT]G 998 CCGCTG MspI 708 CCGG C^CGG 888 CCGG NdeII 423 GATC ^GATC 646 GATC NspI 338 GCATGT [GA]CATG^[CT] 356 GCATGC PstI 502 CTGCAG CTGCA^G 992 CTGCAG Sau3AI 423 GATC ^GATC 646 GATC SfcI 502 CTGCAG C^T[GA][CT]AG 992 CTGCAG SmlI 164 CTCGAG C^T[CT][GA]AG 915 CTCAAG Tru9I 401 TTAA T^TAA 1044 TTAA Tsp45I 256 GTGAC ^GT[GC]AC 762 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AfaI AluI AlwNI BbvI BsaJI BsiYI BslI BsmAI BsmFI BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV PleI RsaI Sau96I ScrFI TaqI TfiI TseI TspRI TthHB8I