##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanII BbeI BbrPI BbvCI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI BstBI BstEII BstPI
BstUI BstXI BstYI ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tsp509I Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 862 GACGTC GACGT^C
AccI 670 GTATAC GT^[AC][GT]AC
AcyI 862 GACGTC G[GA]^CG[CT]C
AocI 961 CCTCAGG CC^T[ACGT]AGG
Aor51HI 851 AGCGCT AGC^GCT
BalI 345 TGGCCA TGG^CCA
BlpI 351 GCTGAGC GC^T[ACGT]AGC
BmyI 564 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 351 GCTGAGC GC^T[ACGT]AGC
BsaAI 583 TACGTA [CT]AC^GT[GA]
BsaHI 862 GACGTC G[GA]^CG[CT]C
BseRI 324 CTCCTC GAGGAG(10/8)
BsmI 179 GCATTC GAATGC(1/-1)
Bsp1286I 564 GGGCAC G[AGT]GC[ACT]^C
BsrDI 83 CATTGC GCAATG(2/0)
Bst1107I 670 GTATAC GTA^TAC
BstZ17I 670 GTATAC GTA^TAC
Bsu36I 961 CCTCAGG CC^T[ACGT]AGG
CelII 351 GCTGAGC GC^T[ACGT]AGC
DraI 1043 TTTAAA TTT^AAA
DraII 770 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco47III 851 AGCGCT AGC^GCT
Eco81I 961 CCTCAGG CC^T[ACGT]AGG
EcoNI 810 CCTGATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 770 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 553 GATATC GAT^ATC
FauI 806 CCCGC CCCGC(4/6)
HaeII 851 AGCGCT [GA]GCGC^[CT]
Hin1I 862 GACGTC G[GA]^CG[CT]C
HincII 1155 GTTGAC GT[CT]^[GA]AC
HindII 1155 GTTGAC GT[CT]^[GA]AC
MluNI 345 TGGCCA TGG^CCA
MscI 345 TGGCCA TGG^CCA
PaeR7I 164 CTCGAG C^TCGAG
PpuMI 770 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 349 CAGCTG CAG^CTG
SapI 876 GCTCTTC GCTCTTC(1/4)
ScaI 299 AGTACT AGT^ACT
SnaBI 583 TACGTA TAC^GTA
SphI 356 GCATGC GCATG^C
XhoI 164 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AvaI 164 CTCGAG C^[CT]CG[GA]G
832 CTCGGG
AvaII 542 GGTCC G^G[AT]CC
771 GGACC
BanI 565 GGCACC G^G[CT][GA]CC
1164 GGCACC
BbsI 141 GAAGAC GAAGAC(2/6)
897 GTCTTC
BcgI 152 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
504 GCAGCTTTATCG
BfaI 329 CTAG C^TAG
1061 CTAG
BpmI 547 CTCCAG CTGGAG(16/14)
453 CTGGAG
BpuAI 141 GAAGAC GAAGAC(2/6)
897 GTCTTC
BsaI 487 GGTCTC GGTCTC(1/5)
1298 GGTCTC
BsgI 947 GTGCAG GTGCAG(16/14)
1119 GTGCAG
BsoBI 164 CTCGAG C^[CT]CG[GA]G
832 CTCGGG
BsrFI 707 GCCGGT [GA]^CCGG[CT]
887 GCCGGT
BstAPI 356 GCATGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
994 GCAGCCGCTGC
Cfr10I 707 GCCGGT [GA]^CCGG[CT]
887 GCCGGT
DpnII 423 GATC ^GATC
646 GATC
EaeI 345 TGGCCA [CT]^GGCC[GA]
381 TGGCCG
EarI 877 CTCTTC CTCTTC(1/4)
625 GAAGAG
HapII 708 CCGG C^CGG
888 CCGG
HgaI 682 GACGC GACGC(5/10)
1158 GACGC
HpaII 708 CCGG C^CGG
888 CCGG
HphI 147 GGTGA GGTGA(8/7)
1307 TCACC
Ksp632I 877 CTCTTC CTCTTC(1/4)
625 GAAGAG
MaeI 329 CTAG C^TAG
1061 CTAG
MaeII 584 ACGT A^CGT
863 ACGT
MboI 423 GATC ^GATC
646 GATC
MseI 401 TTAA T^TAA
1044 TTAA
MslI 333 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1017 CATTTTGATG
MspA1I 349 CAGCTG C[AC]G^C[GT]G
998 CCGCTG
MspI 708 CCGG C^CGG
888 CCGG
NdeII 423 GATC ^GATC
646 GATC
NspI 338 GCATGT [GA]CATG^[CT]
356 GCATGC
PstI 502 CTGCAG CTGCA^G
992 CTGCAG
Sau3AI 423 GATC ^GATC
646 GATC
SfcI 502 CTGCAG C^T[GA][CT]AG
992 CTGCAG
SmlI 164 CTCGAG C^T[CT][GA]AG
915 CTCAAG
Tru9I 401 TTAA T^TAA
1044 TTAA
Tsp45I 256 GTGAC ^GT[GC]AC
762 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AluI AlwNI BbvI
BsaJI BsiYI BslI BsmAI BsmFI
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI ItaI MaeIII MboII MnlI
MvaI MwoI NlaIII NlaIV PleI
RsaI Sau96I ScrFI TaqI TfiI
TseI TspRI TthHB8I