##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbvCI BclI BfrI BglI
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BspEI BspLU11I BsrDI
BsrFI Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DrdI EarI Eco81I EcoO65I EcoRV
FbaI FseI HgaI KpnI Ksp632I
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI SexAI SfiI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tth111I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 2121 GTCTAC GT^[AC][GT]AC
AcyI 109 GGCGCC G[GA]^CG[CT]C
AflIII 1223 ACACGT A^C[GA][CT]GT
Aor51HI 52 AGCGCT AGC^GCT
ApaI 77 GGGCCC GGGCC^C
AvaI 213 CCCGAG C^[CT]CG[GA]G
AviII 1522 TGCGCA TGC^GCA
BalI 1208 TGGCCA TGG^CCA
BamHI 550 GGATCC G^GATCC
BanI 109 GGCGCC G^G[CT][GA]CC
BbeI 109 GGCGCC GGCGC^C
BbrPI 1224 CACGTG CAC^GTG
BsaHI 109 GGCGCC G[GA]^CG[CT]C
BsaI 240 GAGACC GGTCTC(1/5)
BsiEI 6 CGGCCG CG[GA][CT]^CG
BsmFI 1446 GTCCC GGGAC(10/14)
BsoBI 213 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2279 TGTACA T^GTACA
BspHI 1843 TCATGA T^CATGA
BsrBI 1974 CCGCTC CCGCTC(-3/-3)
BsrGI 2279 TGTACA T^GTACA
BssSI 1557 CACGAG CACGAG(-5/-1)
BstAPI 1037 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1777 TTCGAA TT^CGAA
DraI 2242 TTTAAA TTT^AAA
DraIII 1943 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 6 CGGCCG C^GGCCG
EclXI 6 CGGCCG C^GGCCG
Eco47III 52 AGCGCT AGC^GCT
Eco52I 6 CGGCCG C^GGCCG
EcoRI 410 GAATTC G^AATTC
FauI 1147 GCGGG CCCGC(4/6)
FspI 1522 TGCGCA TGC^GCA
Hin1I 109 GGCGCC G[GA]^CG[CT]C
HindIII 846 AAGCTT A^AGCTT
HpaI 899 GTTAAC GTT^AAC
KasI 109 GGCGCC G^GCGCC
KspI 162 CCGCGG CCGC^GG
MluNI 1208 TGGCCA TGG^CCA
MscI 1208 TGGCCA TGG^CCA
NarI 109 GGCGCC GG^CGCC
NheI 2141 GCTAGC G^CTAGC
NspI 1393 ACATGC [GA]CATG^[CT]
NspV 1777 TTCGAA TT^CGAA
PflMI 1430 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1224 CACGTG CAC^GTG
PmlI 1224 CACGTG CAC^GTG
RcaI 1843 TCATGA T^CATGA
SacI 1164 GAGCTC GAGCT^C
SacII 162 CCGCGG CCGC^GG
ScaI 601 AGTACT AGT^ACT
SfoI 109 GGCGCC GGC^GCC
SfuI 1777 TTCGAA TT^CGAA
SspBI 2279 TGTACA T^GTACA
Tsp45I 813 GTGAC ^GT[GC]AC
Van91I 1430 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 410 GAATTC [GA]^AATT[CT]
1488 AAATTC
AhdI 738 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1233 GACCCTCAGTC
AlwNI 498 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
518 CAGCACCTG
ApoI 410 GAATTC [GA]^AATT[CT]
1488 AAATTC
AspEI 738 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1233 GACCCTCAGTC
AvaII 1150 GGTCC G^G[AT]CC
1785 GGACC
BbsI 735 GAAGAC GAAGAC(2/6)
1854 GAAGAC
BciVI 271 GTATCC GTATCC(6/5)
1764 GTATCC
BcnI 123 CCGGG CC^[GC]GG
761 CCCGG
BpuAI 735 GAAGAC GAAGAC(2/6)
1854 GAAGAC
BsaAI 1131 TACGTG [CT]AC^GT[GA]
1224 CACGTG
BsmBI 98 GAGACG CGTCTC(1/5)
201 GAGACG
BsmI 986 GAATGC GAATGC(1/-1)
1639 GAATGC
BspDI 642 ATCGAT AT^CGAT
952 ATCGAT
BspMI 522 ACCTGC ACCTGC(4/8)
20 GCAGGT
BssHII 14 GCGCGC G^CGCGC
16 GCGCGC
ClaI 642 ATCGAT AT^CGAT
952 ATCGAT
Eam1105I 738 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1233 GACCCTCAGTC
EcoNI 39 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1714 CCTCTAAGAGG
EcoT22I 1090 ATGCAT ATGCA^T
1387 ATGCAT
HincII 453 GTCAAC GT[CT]^[GA]AC
899 GTTAAC
HindII 453 GTCAAC GT[CT]^[GA]AC
899 GTTAAC
MaeIII 628 GTAAC ^GT[ACGT]AC
813 GTGAC
MslI 2147 CATGGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2271 CATATATGTG
NciI 123 CCGGG CC^[GC]GG
761 CCCGG
NcoI 554 CCATGG C^CATGG
2146 CCATGG
NsiI 1090 ATGCAT ATGCA^T
1387 ATGCAT
PpuMI 1149 GGGTCCT [GA]G^G[AT]CC[CT]
1784 AGGACCT
PvuII 171 CAGCTG CAG^CTG
1038 CAGCTG
XbaI 1115 TCTAGA T^CTAGA
1322 TCTAGA
XcmI 1634 CCATTGAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2137 CCAGGCTAGCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BanII BbvI BcgI BfaI
BglII BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII