################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvrII BbvCI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiWI BspEI BspLU11I BsrDI BsrFI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DrdI EarI Eco81I EcoO65I EcoRV FbaI FseI HgaI KpnI Ksp632I MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SexAI SfiI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 2121 GTCTAC GT^[AC][GT]AC AcyI 109 GGCGCC G[GA]^CG[CT]C AflIII 1223 ACACGT A^C[GA][CT]GT Aor51HI 52 AGCGCT AGC^GCT ApaI 77 GGGCCC GGGCC^C AvaI 213 CCCGAG C^[CT]CG[GA]G AviII 1522 TGCGCA TGC^GCA BalI 1208 TGGCCA TGG^CCA BamHI 550 GGATCC G^GATCC BanI 109 GGCGCC G^G[CT][GA]CC BbeI 109 GGCGCC GGCGC^C BbrPI 1224 CACGTG CAC^GTG BsaHI 109 GGCGCC G[GA]^CG[CT]C BsaI 240 GAGACC GGTCTC(1/5) BsiEI 6 CGGCCG CG[GA][CT]^CG BsmFI 1446 GTCCC GGGAC(10/14) BsoBI 213 CCCGAG C^[CT]CG[GA]G Bsp1407I 2279 TGTACA T^GTACA BspHI 1843 TCATGA T^CATGA BsrBI 1974 CCGCTC CCGCTC(-3/-3) BsrGI 2279 TGTACA T^GTACA BssSI 1557 CACGAG CACGAG(-5/-1) BstAPI 1037 GCAGCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1777 TTCGAA TT^CGAA DraI 2242 TTTAAA TTT^AAA DraIII 1943 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 6 CGGCCG C^GGCCG EclXI 6 CGGCCG C^GGCCG Eco47III 52 AGCGCT AGC^GCT Eco52I 6 CGGCCG C^GGCCG EcoRI 410 GAATTC G^AATTC FauI 1147 GCGGG CCCGC(4/6) FspI 1522 TGCGCA TGC^GCA Hin1I 109 GGCGCC G[GA]^CG[CT]C HindIII 846 AAGCTT A^AGCTT HpaI 899 GTTAAC GTT^AAC KasI 109 GGCGCC G^GCGCC KspI 162 CCGCGG CCGC^GG MluNI 1208 TGGCCA TGG^CCA MscI 1208 TGGCCA TGG^CCA NarI 109 GGCGCC GG^CGCC NheI 2141 GCTAGC G^CTAGC NspI 1393 ACATGC [GA]CATG^[CT] NspV 1777 TTCGAA TT^CGAA PflMI 1430 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1224 CACGTG CAC^GTG PmlI 1224 CACGTG CAC^GTG RcaI 1843 TCATGA T^CATGA SacI 1164 GAGCTC GAGCT^C SacII 162 CCGCGG CCGC^GG ScaI 601 AGTACT AGT^ACT SfoI 109 GGCGCC GGC^GCC SfuI 1777 TTCGAA TT^CGAA SspBI 2279 TGTACA T^GTACA Tsp45I 813 GTGAC ^GT[GC]AC Van91I 1430 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcsI 410 GAATTC [GA]^AATT[CT] 1488 AAATTC AhdI 738 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1233 GACCCTCAGTC AlwNI 498 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 518 CAGCACCTG ApoI 410 GAATTC [GA]^AATT[CT] 1488 AAATTC AspEI 738 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1233 GACCCTCAGTC AvaII 1150 GGTCC G^G[AT]CC 1785 GGACC BbsI 735 GAAGAC GAAGAC(2/6) 1854 GAAGAC BciVI 271 GTATCC GTATCC(6/5) 1764 GTATCC BcnI 123 CCGGG CC^[GC]GG 761 CCCGG BpuAI 735 GAAGAC GAAGAC(2/6) 1854 GAAGAC BsaAI 1131 TACGTG [CT]AC^GT[GA] 1224 CACGTG BsmBI 98 GAGACG CGTCTC(1/5) 201 GAGACG BsmI 986 GAATGC GAATGC(1/-1) 1639 GAATGC BspDI 642 ATCGAT AT^CGAT 952 ATCGAT BspMI 522 ACCTGC ACCTGC(4/8) 20 GCAGGT BssHII 14 GCGCGC G^CGCGC 16 GCGCGC ClaI 642 ATCGAT AT^CGAT 952 ATCGAT Eam1105I 738 GACACTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1233 GACCCTCAGTC EcoNI 39 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1714 CCTCTAAGAGG EcoT22I 1090 ATGCAT ATGCA^T 1387 ATGCAT HincII 453 GTCAAC GT[CT]^[GA]AC 899 GTTAAC HindII 453 GTCAAC GT[CT]^[GA]AC 899 GTTAAC MaeIII 628 GTAAC ^GT[ACGT]AC 813 GTGAC MslI 2147 CATGGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2271 CATATATGTG NciI 123 CCGGG CC^[GC]GG 761 CCCGG NcoI 554 CCATGG C^CATGG 2146 CCATGG NsiI 1090 ATGCAT ATGCA^T 1387 ATGCAT PpuMI 1149 GGGTCCT [GA]G^G[AT]CC[CT] 1784 AGGACCT PvuII 171 CAGCTG CAG^CTG 1038 CAGCTG XbaI 1115 TCTAGA T^CTAGA 1322 TCTAGA XcmI 1634 CCATTGAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2137 CCAGGCTAGCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI BanII BbvI BcgI BfaI BglII BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp509I TspRI TthHB8I XhoII