##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        Alw44I      AlwI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AvrII       BamHI       
BanI        BbeI        BcgI        BclI        BfaI        
BfrI        BglII       BlnI        BsaBI       BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     ClaI        CpoI        DpnII       DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MboI        MflI        MluI        MroI        
MslI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        Sau3AI      ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SspBI       SspI        SwaI        Tth111I     
XbaI        XcmI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII          20  CGCG                 CG^CG
AflIII       2113  ACACGT               A^C[GA][CT]GT
AhdI         2112  GACACGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        2112  GACACGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         973  TGCGCA               TGC^GCA
BbrPI        2114  CACGTG               CAC^GTG
BbvCI         350  CCTCAGC              CCTCAGC(-5/-2)
BciVI           4  GGATAC               GTATCC(6/5)
BlpI          461  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      461  GCTAAGC              GC^T[ACGT]AGC
BsaAI        2114  CACGTG               [CT]AC^GT[GA]
BsmBI        1066  CGTCTC               CGTCTC(1/5)
BstUI          20  CGCG                 CG^CG
CelII         461  GCTAAGC              GC^T[ACGT]AGC
DraI         2311  TTTAAA               TTT^AAA
Eam1105I     2112  GACACGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI          973  TGCGCA               TGC^GCA
HaeII         113  GGCGCT               [GA]GCGC^[CT]
HindIII       378  AAGCTT               A^AGCTT
MfeI         2334  CAATTG               C^AATTG
MunI         2334  CAATTG               C^AATTG
MvnI           20  CGCG                 CG^CG
NspI          355  GCATGC               [GA]CATG^[CT]
PflMI        1520  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        2114  CACGTG               CAC^GTG
PmlI         2114  CACGTG               CAC^GTG
PshAI        1060  GACGGTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         1250  CCTGCAGG             CCTGCA^GG
SexAI         625  ACCAGGT              A^CC[AT]GGT
SfaNI        1946  GATGC                GCATC(5/9)
SmaI          943  CCCGGG               CCC^GGG
SphI          355  GCATGC               GCATG^C
Sse8387I     1250  CCTGCAGG             CCTGCA^GG
StuI         1335  AGGCCT               AGG^CCT
Van91I       1520  CCAGCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         943  CCCGGG               C^CCGGG
XmaI          943  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI           95  AAATTT               [GA]^AATT[CT]
             2096  AAATTC              
AcyI          128  GACGCC               G[GA]^CG[CT]C
             1873  GGCGTC              
ApoI           95  AAATTT               [GA]^AATT[CT]
             2096  AAATTC              
BalI           87  TGGCCA               TGG^CCA
              780  TGGCCA              
BanII         508  GAGCTC               G[GA]GC[CT]^C
              823  GAGCTC              
BglI         1394  GCCAAACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1403  GCCCCTCAGGC         
BsaHI         128  GACGCC               G[GA]^CG[CT]C
             1873  GGCGTC              
BsaI         1781  GAGACC               GGTCTC(1/5)
             1829  GAGACC              
BsgI          741  CTGCAC               GTGCAG(16/14)
             1959  GTGCAG              
BsiEI         108  CGACCG               CG[GA][CT]^CG
             1062  CGGTCG              
BsrFI           8  ACCGGC               [GA]^CCGG[CT]
              110  ACCGGC              
Cfr10I          8  ACCGGC               [GA]^CCGG[CT]
              110  ACCGGC              
EaeI           87  TGGCCA               [CT]^GGCC[GA]
              780  TGGCCA              
HgaI          128  GACGC                GACGC(5/10)
             1874  GCGTC               
Hin1I         128  GACGCC               G[GA]^CG[CT]C
             1873  GGCGTC              
MaeII        1841  ACGT                 A^CGT
             2115  ACGT                
MluNI          87  TGGCCA               TGG^CCA
              780  TGGCCA              
MscI           87  TGGCCA               TGG^CCA
              780  TGGCCA              
PaeR7I       1826  CTCGAG               C^TCGAG
             1923  CTCGAG              
PpuMI         892  AGGTCCC              [GA]G^G[AT]CC[CT]
             2226  AGGTCCT             
SacI          508  GAGCTC               GAGCT^C
              823  GAGCTC              
SapI          820  GAAGAGC              GCTCTTC(1/4)
             1116  GCTCTTC             
XhoI         1826  CTCGAG               C^TCGAG
             1923  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AocI        
AspHI       AvaI        AvaII       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DraII       EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboII       MnlI        MseI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       PleI        PstI        PvuII       
RsaI        Sau96I      ScrFI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I