##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BamHI
BanI BbeI BcgI BclI BfaI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I ClaI CpoI DpnII DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KasI KpnI KspI MaeI
MamI MboI MflI MluI MroI
MslI NaeI NarI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI Sau3AI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 20 CGCG CG^CG
AflIII 2113 ACACGT A^C[GA][CT]GT
AhdI 2112 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2112 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 973 TGCGCA TGC^GCA
BbrPI 2114 CACGTG CAC^GTG
BbvCI 350 CCTCAGC CCTCAGC(-5/-2)
BciVI 4 GGATAC GTATCC(6/5)
BlpI 461 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 461 GCTAAGC GC^T[ACGT]AGC
BsaAI 2114 CACGTG [CT]AC^GT[GA]
BsmBI 1066 CGTCTC CGTCTC(1/5)
BstUI 20 CGCG CG^CG
CelII 461 GCTAAGC GC^T[ACGT]AGC
DraI 2311 TTTAAA TTT^AAA
Eam1105I 2112 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 973 TGCGCA TGC^GCA
HaeII 113 GGCGCT [GA]GCGC^[CT]
HindIII 378 AAGCTT A^AGCTT
MfeI 2334 CAATTG C^AATTG
MunI 2334 CAATTG C^AATTG
MvnI 20 CGCG CG^CG
NspI 355 GCATGC [GA]CATG^[CT]
PflMI 1520 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2114 CACGTG CAC^GTG
PmlI 2114 CACGTG CAC^GTG
PshAI 1060 GACGGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1250 CCTGCAGG CCTGCA^GG
SexAI 625 ACCAGGT A^CC[AT]GGT
SfaNI 1946 GATGC GCATC(5/9)
SmaI 943 CCCGGG CCC^GGG
SphI 355 GCATGC GCATG^C
Sse8387I 1250 CCTGCAGG CCTGCA^GG
StuI 1335 AGGCCT AGG^CCT
Van91I 1520 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 943 CCCGGG C^CCGGG
XmaI 943 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 95 AAATTT [GA]^AATT[CT]
2096 AAATTC
AcyI 128 GACGCC G[GA]^CG[CT]C
1873 GGCGTC
ApoI 95 AAATTT [GA]^AATT[CT]
2096 AAATTC
BalI 87 TGGCCA TGG^CCA
780 TGGCCA
BanII 508 GAGCTC G[GA]GC[CT]^C
823 GAGCTC
BglI 1394 GCCAAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1403 GCCCCTCAGGC
BsaHI 128 GACGCC G[GA]^CG[CT]C
1873 GGCGTC
BsaI 1781 GAGACC GGTCTC(1/5)
1829 GAGACC
BsgI 741 CTGCAC GTGCAG(16/14)
1959 GTGCAG
BsiEI 108 CGACCG CG[GA][CT]^CG
1062 CGGTCG
BsrFI 8 ACCGGC [GA]^CCGG[CT]
110 ACCGGC
Cfr10I 8 ACCGGC [GA]^CCGG[CT]
110 ACCGGC
EaeI 87 TGGCCA [CT]^GGCC[GA]
780 TGGCCA
HgaI 128 GACGC GACGC(5/10)
1874 GCGTC
Hin1I 128 GACGCC G[GA]^CG[CT]C
1873 GGCGTC
MaeII 1841 ACGT A^CGT
2115 ACGT
MluNI 87 TGGCCA TGG^CCA
780 TGGCCA
MscI 87 TGGCCA TGG^CCA
780 TGGCCA
PaeR7I 1826 CTCGAG C^TCGAG
1923 CTCGAG
PpuMI 892 AGGTCCC [GA]G^G[AT]CC[CT]
2226 AGGTCCT
SacI 508 GAGCTC GAGCT^C
823 GAGCTC
SapI 820 GAAGAGC GCTCTTC(1/4)
1116 GCTCTTC
XhoI 1826 CTCGAG C^TCGAG
1923 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
AspHI AvaI AvaII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI DraII EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV PleI PstI PvuII
RsaI Sau96I ScrFI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I