################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvrII BamHI BanI BbeI BcgI BclI BfaI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DpnII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI KasI KpnI KspI MaeI MamI MboI MflI MluI MroI MslI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI Sau3AI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 20 CGCG CG^CG AflIII 2113 ACACGT A^C[GA][CT]GT AhdI 2112 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2112 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 973 TGCGCA TGC^GCA BbrPI 2114 CACGTG CAC^GTG BbvCI 350 CCTCAGC CCTCAGC(-5/-2) BciVI 4 GGATAC GTATCC(6/5) BlpI 461 GCTAAGC GC^T[ACGT]AGC Bpu1102I 461 GCTAAGC GC^T[ACGT]AGC BsaAI 2114 CACGTG [CT]AC^GT[GA] BsmBI 1066 CGTCTC CGTCTC(1/5) BstUI 20 CGCG CG^CG CelII 461 GCTAAGC GC^T[ACGT]AGC DraI 2311 TTTAAA TTT^AAA Eam1105I 2112 GACACGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FspI 973 TGCGCA TGC^GCA HaeII 113 GGCGCT [GA]GCGC^[CT] HindIII 378 AAGCTT A^AGCTT MfeI 2334 CAATTG C^AATTG MunI 2334 CAATTG C^AATTG MvnI 20 CGCG CG^CG NspI 355 GCATGC [GA]CATG^[CT] PflMI 1520 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2114 CACGTG CAC^GTG PmlI 2114 CACGTG CAC^GTG PshAI 1060 GACGGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 1250 CCTGCAGG CCTGCA^GG SexAI 625 ACCAGGT A^CC[AT]GGT SfaNI 1946 GATGC GCATC(5/9) SmaI 943 CCCGGG CCC^GGG SphI 355 GCATGC GCATG^C Sse8387I 1250 CCTGCAGG CCTGCA^GG StuI 1335 AGGCCT AGG^CCT Van91I 1520 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 943 CCCGGG C^CCGGG XmaI 943 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 95 AAATTT [GA]^AATT[CT] 2096 AAATTC AcyI 128 GACGCC G[GA]^CG[CT]C 1873 GGCGTC ApoI 95 AAATTT [GA]^AATT[CT] 2096 AAATTC BalI 87 TGGCCA TGG^CCA 780 TGGCCA BanII 508 GAGCTC G[GA]GC[CT]^C 823 GAGCTC BglI 1394 GCCAAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1403 GCCCCTCAGGC BsaHI 128 GACGCC G[GA]^CG[CT]C 1873 GGCGTC BsaI 1781 GAGACC GGTCTC(1/5) 1829 GAGACC BsgI 741 CTGCAC GTGCAG(16/14) 1959 GTGCAG BsiEI 108 CGACCG CG[GA][CT]^CG 1062 CGGTCG BsrFI 8 ACCGGC [GA]^CCGG[CT] 110 ACCGGC Cfr10I 8 ACCGGC [GA]^CCGG[CT] 110 ACCGGC EaeI 87 TGGCCA [CT]^GGCC[GA] 780 TGGCCA HgaI 128 GACGC GACGC(5/10) 1874 GCGTC Hin1I 128 GACGCC G[GA]^CG[CT]C 1873 GGCGTC MaeII 1841 ACGT A^CGT 2115 ACGT MluNI 87 TGGCCA TGG^CCA 780 TGGCCA MscI 87 TGGCCA TGG^CCA 780 TGGCCA PaeR7I 1826 CTCGAG C^TCGAG 1923 CTCGAG PpuMI 892 AGGTCCC [GA]G^G[AT]CC[CT] 2226 AGGTCCT SacI 508 GAGCTC GAGCT^C 823 GAGCTC SapI 820 GAAGAGC GCTCTTC(1/4) 1116 GCTCTTC XhoI 1826 CTCGAG C^TCGAG 1923 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AluI AlwNI AocI AspHI AvaI AvaII BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DraII EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI PstI PvuII RsaI Sau96I ScrFI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I