##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FspI Hin1I HincII
HindII HpaI KasI KspI MaeII
MamI MfeI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SmlI SnaBI
SpeI SspBI StuI SwaI Tru9I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 977 GGTACC G^GTACC
AflIII 840 ACGCGT A^C[GA][CT]GT
AgeI 295 ACCGGT A^CCGGT
AlwI 433 GATCC GGATC(4/5)
AlwNI 509 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1336 GGGCCC GGGCC^C
Asp718I 977 GGTACC G^GTACC
AspHI 449 GAGCTC G[AT]GC[AT]^C
BglI 1116 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 871 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 871 GCTAAGC GC^T[ACGT]AGC
BsaWI 295 ACCGGT [AT]^CCGG[AT]
BseRI 5 GAGGAG GAGGAG(10/8)
BsiHKAI 449 GAGCTC G[AT]GC[AT]^C
BsmFI 476 GTCCC GGGAC(10/14)
BspDI 1007 ATCGAT AT^CGAT
BspMI 960 ACCTGC ACCTGC(4/8)
BssSI 1091 CACGAG CACGAG(-5/-1)
BstXI 649 CCAGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 432 AGATCC [GA]^GATC[CT]
CelII 871 GCTAAGC GC^T[ACGT]AGC
CfoI 250 GCGC GCG^C
ClaI 1007 ATCGAT AT^CGAT
DraII 1335 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 130 GACAGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1111 CCATGG C^C[GA][CT]GG
EarI 2 GAAGAG CTCTTC(1/4)
EcoO109I 1335 GGGGCCC [GA]G^G[ACGT]CC[CT]
FseI 58 GGCCGGCC GGCCGG^CC
HaeII 249 GGCGCT [GA]GCGC^[CT]
HgaI 839 GACGC GACGC(5/10)
HhaI 250 GCGC GCG^C
HinP1I 250 GCGC G^CGC
HindIII 1356 AAGCTT A^AGCTT
KpnI 977 GGTACC GGTAC^C
Ksp632I 2 GAAGAG CTCTTC(1/4)
MflI 432 AGATCC [GA]^GATC[CT]
MluI 840 ACGCGT A^CGCGT
NcoI 1111 CCATGG C^CATGG
NspI 410 GCATGC [GA]CATG^[CT]
PinAI 295 ACCGGT A^CCGGT
SacI 449 GAGCTC GAGCT^C
SbfI 599 CCTGCAGG CCTGCA^GG
SgrAI 294 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1339 CCCGGG CCC^GGG
SphI 410 GCATGC GCATG^C
Sse8387I 599 CCTGCAGG CCTGCA^GG
SspI 1450 AATATT AAT^ATT
TaqI 1008 TCGA T^CGA
Tsp509I 1488 AATT ^AATT
TthHB8I 1008 TCGA T^CGA
XhoII 432 AGATCC [GA]^GATC[CT]
XmaCI 1339 CCCGGG C^CCGGG
XmaI 1339 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 105 CGCG CG^CG
841 CGCG
AfaI 881 GTAC GT^AC
978 GTAC
AocI 13 CCTCAGG CC^T[ACGT]AGG
716 CCTGAGG
AvaII 1207 GGACC G^G[AT]CC
1257 GGTCC
BanII 449 GAGCTC G[GA]GC[CT]^C
1336 GGGCCC
BbsI 1245 GAAGAC GAAGAC(2/6)
524 GTCTTC
BpuAI 1245 GAAGAC GAAGAC(2/6)
524 GTCTTC
BstEII 31 GGTCACC G^GT[ACGT]ACC
988 GGTAACC
BstPI 31 GGTCACC G^GT[ACGT]ACC
988 GGTAACC
BstUI 105 CGCG CG^CG
841 CGCG
Bsu36I 13 CCTCAGG CC^T[ACGT]AGG
716 CCTGAGG
Eco81I 13 CCTCAGG CC^T[ACGT]AGG
716 CCTGAGG
EcoO65I 31 GGTCACC G^GT[ACGT]ACC
988 GGTAACC
MslI 416 CATGCGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1145 CATCCAGGTG
MvnI 105 CGCG CG^CG
841 CGCG
NaeI 59 GCCGGC GCC^GGC
765 GCCGGC
NgoMIV 59 GCCGGC G^CCGGC
765 GCCGGC
PflMI 183 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
322 CCAGGCTGTGG
PstI 600 CTGCAG CTGCA^G
1270 CTGCAG
RsaI 881 GTAC GT^AC
978 GTAC
Van91I 183 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
322 CCAGGCTGTGG
XcmI 1111 CCATGGCCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1322 CCAGGAGATTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AvaI BalI BanI
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsgI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I TspRI