################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FspI Hin1I HincII HindII HpaI KasI KspI MaeII MamI MfeI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SmlI SnaBI SpeI SspBI StuI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 977 GGTACC G^GTACC AflIII 840 ACGCGT A^C[GA][CT]GT AgeI 295 ACCGGT A^CCGGT AlwI 433 GATCC GGATC(4/5) AlwNI 509 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1336 GGGCCC GGGCC^C Asp718I 977 GGTACC G^GTACC AspHI 449 GAGCTC G[AT]GC[AT]^C BglI 1116 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 871 GCTAAGC GC^T[ACGT]AGC Bpu1102I 871 GCTAAGC GC^T[ACGT]AGC BsaWI 295 ACCGGT [AT]^CCGG[AT] BseRI 5 GAGGAG GAGGAG(10/8) BsiHKAI 449 GAGCTC G[AT]GC[AT]^C BsmFI 476 GTCCC GGGAC(10/14) BspDI 1007 ATCGAT AT^CGAT BspMI 960 ACCTGC ACCTGC(4/8) BssSI 1091 CACGAG CACGAG(-5/-1) BstXI 649 CCAGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 432 AGATCC [GA]^GATC[CT] CelII 871 GCTAAGC GC^T[ACGT]AGC CfoI 250 GCGC GCG^C ClaI 1007 ATCGAT AT^CGAT DraII 1335 GGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 130 GACAGGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1111 CCATGG C^C[GA][CT]GG EarI 2 GAAGAG CTCTTC(1/4) EcoO109I 1335 GGGGCCC [GA]G^G[ACGT]CC[CT] FseI 58 GGCCGGCC GGCCGG^CC HaeII 249 GGCGCT [GA]GCGC^[CT] HgaI 839 GACGC GACGC(5/10) HhaI 250 GCGC GCG^C HinP1I 250 GCGC G^CGC HindIII 1356 AAGCTT A^AGCTT KpnI 977 GGTACC GGTAC^C Ksp632I 2 GAAGAG CTCTTC(1/4) MflI 432 AGATCC [GA]^GATC[CT] MluI 840 ACGCGT A^CGCGT NcoI 1111 CCATGG C^CATGG NspI 410 GCATGC [GA]CATG^[CT] PinAI 295 ACCGGT A^CCGGT SacI 449 GAGCTC GAGCT^C SbfI 599 CCTGCAGG CCTGCA^GG SgrAI 294 CACCGGTG C[GA]^CCGG[CT]G SmaI 1339 CCCGGG CCC^GGG SphI 410 GCATGC GCATG^C Sse8387I 599 CCTGCAGG CCTGCA^GG SspI 1450 AATATT AAT^ATT TaqI 1008 TCGA T^CGA Tsp509I 1488 AATT ^AATT TthHB8I 1008 TCGA T^CGA XhoII 432 AGATCC [GA]^GATC[CT] XmaCI 1339 CCCGGG C^CCGGG XmaI 1339 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 105 CGCG CG^CG 841 CGCG AfaI 881 GTAC GT^AC 978 GTAC AocI 13 CCTCAGG CC^T[ACGT]AGG 716 CCTGAGG AvaII 1207 GGACC G^G[AT]CC 1257 GGTCC BanII 449 GAGCTC G[GA]GC[CT]^C 1336 GGGCCC BbsI 1245 GAAGAC GAAGAC(2/6) 524 GTCTTC BpuAI 1245 GAAGAC GAAGAC(2/6) 524 GTCTTC BstEII 31 GGTCACC G^GT[ACGT]ACC 988 GGTAACC BstPI 31 GGTCACC G^GT[ACGT]ACC 988 GGTAACC BstUI 105 CGCG CG^CG 841 CGCG Bsu36I 13 CCTCAGG CC^T[ACGT]AGG 716 CCTGAGG Eco81I 13 CCTCAGG CC^T[ACGT]AGG 716 CCTGAGG EcoO65I 31 GGTCACC G^GT[ACGT]ACC 988 GGTAACC MslI 416 CATGCGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1145 CATCCAGGTG MvnI 105 CGCG CG^CG 841 CGCG NaeI 59 GCCGGC GCC^GGC 765 GCCGGC NgoMIV 59 GCCGGC G^CCGGC 765 GCCGGC PflMI 183 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 322 CCAGGCTGTGG PstI 600 CTGCAG CTGCA^G 1270 CTGCAG RsaI 881 GTAC GT^AC 978 GTAC Van91I 183 CCAAGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 322 CCAGGCTGTGG XcmI 1111 CCATGGCCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1322 CCAGGAGATTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AluI AvaI BalI BanI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsgI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BsrI BssKI BstAPI BstF5I BstNI Cac8I Cfr10I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI TseI Tsp45I TspRI