################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AvaI AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV FseI HaeII HapII HgaI HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflIII 901 ACACGT A^C[GA][CT]GT AhdI 980 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 1791 ATTAAT AT^TAAT AsnI 1791 ATTAAT AT^TAAT Asp700I 70 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 980 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1261 GAGCAC G[AT]GC[AT]^C AspI 330 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 426 TGCGCA TGC^GCA BamHI 490 GGATCC G^GATCC BanI 243 GGTGCC G^G[CT][GA]CC BclI 176 TGATCA T^GATCA BsaAI 902 CACGTA [CT]AC^GT[GA] BsgI 1521 CTGCAC GTGCAG(16/14) BsiHKAI 1261 GAGCAC G[AT]GC[AT]^C BsrBI 1495 GAGCGG CCGCTC(-3/-3) BsrDI 638 CATTGC GCAATG(2/0) BssSI 982 CTCGTG CACGAG(-5/-1) BstBI 1133 TTCGAA TT^CGAA BstXI 1031 CCACACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 490 GGATCC [GA]^GATC[CT] Cac8I 614 GCTTGC GC[ACGT]^[ACGT]GC CfoI 427 GCGC GCG^C DrdI 1093 GACACCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 973 CCACGG C^C[GA][CT]GG Eam1105I 980 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 289 GAAGAG CTCTTC(1/4) EcoNI 1360 CCTAGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1136 GAATTC G^AATTC EcoT22I 907 ATGCAT ATGCA^T FauI 1465 CCCGC CCCGC(4/6) FbaI 176 TGATCA T^GATCA FspI 426 TGCGCA TGC^GCA HhaI 427 GCGC GCG^C HinP1I 427 GCGC G^CGC HindIII 1154 AAGCTT A^AGCTT Ksp632I 289 GAAGAG CTCTTC(1/4) MflI 490 GGATCC [GA]^GATC[CT] NdeI 193 CATATG CA^TATG NsiI 907 ATGCAT ATGCA^T NspI 909 GCATGT [GA]CATG^[CT] NspV 1133 TTCGAA TT^CGAA PflFI 330 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1791 ATTAAT AT^TAAT SalI 1516 GTCGAC G^TCGAC ScaI 511 AGTACT AGT^ACT SfaNI 1142 GCATC GCATC(5/9) SfuI 1133 TTCGAA TT^CGAA Tth111I 330 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 216 CCAGCTTGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 490 GGATCC [GA]^GATC[CT] XmnI 70 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AatII 500 GACGTC GACGT^C 1670 GACGTC AccI 1090 GTAGAC GT^[AC][GT]AC 1516 GTCGAC AcsI 74 AAATTC [GA]^AATT[CT] 1136 GAATTC AcyI 500 GACGTC G[GA]^CG[CT]C 1670 GACGTC AlwI 491 GATCC GGATC(4/5) 490 GGATC ApoI 74 AAATTC [GA]^AATT[CT] 1136 GAATTC BalI 410 TGGCCA TGG^CCA 856 TGGCCA BbsI 294 GAAGAC GAAGAC(2/6) 1234 GTCTTC BpuAI 294 GAAGAC GAAGAC(2/6) 1234 GTCTTC BsaHI 500 GACGTC G[GA]^CG[CT]C 1670 GACGTC BspMI 550 ACCTGC ACCTGC(4/8) 1558 ACCTGC EaeI 410 TGGCCA [CT]^GGCC[GA] 856 TGGCCA Hin1I 500 GACGTC G[GA]^CG[CT]C 1670 GACGTC HincII 390 GTTGAC GT[CT]^[GA]AC 1516 GTCGAC HindII 390 GTTGAC GT[CT]^[GA]AC 1516 GTCGAC MluNI 410 TGGCCA TGG^CCA 856 TGGCCA MscI 410 TGGCCA TGG^CCA 856 TGGCCA MslI 20 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 565 CATAATTATG PstI 124 CTGCAG CTGCA^G 552 CTGCAG PvuII 121 CAGCTG CAG^CTG 1455 CAGCTG SmlI 153 CTCAAG C^T[CT][GA]AG 348 CTTGAG TfiI 1315 GAATC G^A[AT]TC 1416 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BbvI BfaI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I