##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AvaI AvrII
BanII BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV FseI HaeII HapII
HgaI HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AflIII 901 ACACGT A^C[GA][CT]GT
AhdI 980 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1791 ATTAAT AT^TAAT
AsnI 1791 ATTAAT AT^TAAT
Asp700I 70 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 980 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1261 GAGCAC G[AT]GC[AT]^C
AspI 330 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 426 TGCGCA TGC^GCA
BamHI 490 GGATCC G^GATCC
BanI 243 GGTGCC G^G[CT][GA]CC
BclI 176 TGATCA T^GATCA
BsaAI 902 CACGTA [CT]AC^GT[GA]
BsgI 1521 CTGCAC GTGCAG(16/14)
BsiHKAI 1261 GAGCAC G[AT]GC[AT]^C
BsrBI 1495 GAGCGG CCGCTC(-3/-3)
BsrDI 638 CATTGC GCAATG(2/0)
BssSI 982 CTCGTG CACGAG(-5/-1)
BstBI 1133 TTCGAA TT^CGAA
BstXI 1031 CCACACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 490 GGATCC [GA]^GATC[CT]
Cac8I 614 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 427 GCGC GCG^C
DrdI 1093 GACACCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 973 CCACGG C^C[GA][CT]GG
Eam1105I 980 GACTCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 289 GAAGAG CTCTTC(1/4)
EcoNI 1360 CCTAGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1136 GAATTC G^AATTC
EcoT22I 907 ATGCAT ATGCA^T
FauI 1465 CCCGC CCCGC(4/6)
FbaI 176 TGATCA T^GATCA
FspI 426 TGCGCA TGC^GCA
HhaI 427 GCGC GCG^C
HinP1I 427 GCGC G^CGC
HindIII 1154 AAGCTT A^AGCTT
Ksp632I 289 GAAGAG CTCTTC(1/4)
MflI 490 GGATCC [GA]^GATC[CT]
NdeI 193 CATATG CA^TATG
NsiI 907 ATGCAT ATGCA^T
NspI 909 GCATGT [GA]CATG^[CT]
NspV 1133 TTCGAA TT^CGAA
PflFI 330 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1791 ATTAAT AT^TAAT
SalI 1516 GTCGAC G^TCGAC
ScaI 511 AGTACT AGT^ACT
SfaNI 1142 GCATC GCATC(5/9)
SfuI 1133 TTCGAA TT^CGAA
Tth111I 330 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 216 CCAGCTTGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 490 GGATCC [GA]^GATC[CT]
XmnI 70 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 500 GACGTC GACGT^C
1670 GACGTC
AccI 1090 GTAGAC GT^[AC][GT]AC
1516 GTCGAC
AcsI 74 AAATTC [GA]^AATT[CT]
1136 GAATTC
AcyI 500 GACGTC G[GA]^CG[CT]C
1670 GACGTC
AlwI 491 GATCC GGATC(4/5)
490 GGATC
ApoI 74 AAATTC [GA]^AATT[CT]
1136 GAATTC
BalI 410 TGGCCA TGG^CCA
856 TGGCCA
BbsI 294 GAAGAC GAAGAC(2/6)
1234 GTCTTC
BpuAI 294 GAAGAC GAAGAC(2/6)
1234 GTCTTC
BsaHI 500 GACGTC G[GA]^CG[CT]C
1670 GACGTC
BspMI 550 ACCTGC ACCTGC(4/8)
1558 ACCTGC
EaeI 410 TGGCCA [CT]^GGCC[GA]
856 TGGCCA
Hin1I 500 GACGTC G[GA]^CG[CT]C
1670 GACGTC
HincII 390 GTTGAC GT[CT]^[GA]AC
1516 GTCGAC
HindII 390 GTTGAC GT[CT]^[GA]AC
1516 GTCGAC
MluNI 410 TGGCCA TGG^CCA
856 TGGCCA
MscI 410 TGGCCA TGG^CCA
856 TGGCCA
MslI 20 CACTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
565 CATAATTATG
PstI 124 CTGCAG CTGCA^G
552 CTGCAG
PvuII 121 CAGCTG CAG^CTG
1455 CAGCTG
SmlI 153 CTCAAG C^T[CT][GA]AG
348 CTTGAG
TfiI 1315 GAATC G^A[AT]TC
1416 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I