##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI AocI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BbeI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiWI BsoBI BspDI
BspEI BsrBI BsrDI BssSI Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 881 GTCTAC GT^[AC][GT]AC
AcsI 1685 AAATTC [GA]^AATT[CT]
AgeI 500 ACCGGT A^CCGGT
Alw44I 977 GTGCAC G^TGCAC
AlwNI 1253 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1415 GGGCCC GGGCC^C
ApaLI 977 GTGCAC G^TGCAC
ApoI 1685 AAATTC [GA]^AATT[CT]
AspHI 977 GTGCAC G[AT]GC[AT]^C
BamHI 424 GGATCC G^GATCC
BanII 1415 GGGCCC G[GA]GC[CT]^C
BbrPI 617 CACGTG CAC^GTG
BbvCI 227 CCTCAGC CCTCAGC(-5/-2)
BciVI 534 GTATCC GTATCC(6/5)
BsaWI 500 ACCGGT [AT]^CCGG[AT]
BsgI 736 GTGCAG GTGCAG(16/14)
BsiEI 429 CGACCG CG[GA][CT]^CG
BsiHKAI 977 GTGCAC G[AT]GC[AT]^C
BsmBI 1348 CGTCTC CGTCTC(1/5)
BsmI 1369 GAATGC GAATGC(1/-1)
Bsp1407I 1702 TGTACA T^GTACA
BspHI 1560 TCATGA T^CATGA
BspLU11I 1699 ACATGT A^CATGT
BsrFI 500 ACCGGT [GA]^CCGG[CT]
BsrGI 1702 TGTACA T^GTACA
BssHII 15 GCGCGC G^CGCGC
BstAPI 729 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1688 TTCGAA TT^CGAA
Cfr10I 500 ACCGGT [GA]^CCGG[CT]
DraI 1662 TTTAAA TTT^AAA
DraII 1414 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 1743 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 1414 AGGGCCC [GA]G^G[ACGT]CC[CT]
NcoI 994 CCATGG C^CATGG
NspI 1699 ACATGT [GA]CATG^[CT]
NspV 1688 TTCGAA TT^CGAA
PinAI 500 ACCGGT A^CCGGT
PmaCI 617 CACGTG CAC^GTG
PmlI 617 CACGTG CAC^GTG
RcaI 1560 TCATGA T^CATGA
ScaI 1775 AGTACT AGT^ACT
SfcI 1496 CTACAG C^T[GA][CT]AG
SfuI 1688 TTCGAA TT^CGAA
SspBI 1702 TGTACA T^GTACA
TfiI 1291 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 1699 ACATGT A^C[GA][CT]GT
1705 ACACGT
BmyI 977 GTGCAC G[AGT]GC[ACT]^C
1415 GGGCCC
BsaAI 617 CACGTG [CT]AC^GT[GA]
1706 CACGTA
BsaI 203 GAGACC GGTCTC(1/5)
222 GAGACC
Bsp1286I 977 GTGCAC G[AGT]GC[ACT]^C
1415 GGGCCC
DsaI 112 CCACGG C^C[GA][CT]GG
994 CCATGG
EarI 653 GAAGAG CTCTTC(1/4)
1362 GAAGAG
EcoT14I 626 CCAAGG C^C[AT][AT]GG
994 CCATGG
FauI 684 CCCGC CCCGC(4/6)
1067 GCGGG
Ksp632I 653 GAAGAG CTCTTC(1/4)
1362 GAAGAG
PflMI 365 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1252 CCAGGATCTGG
StyI 626 CCAAGG C^C[AT][AT]GG
994 CCATGG
Van91I 365 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1252 CCAGGATCTGG
XbaI 519 TCTAGA T^CTAGA
774 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaII BbsI BbvI BcnI BfaI
BglI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DrdI EcoNI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII