################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiWI BsoBI BspDI BspEI BsrBI BsrDI BssSI Bst1107I BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 881 GTCTAC GT^[AC][GT]AC AcsI 1685 AAATTC [GA]^AATT[CT] AgeI 500 ACCGGT A^CCGGT Alw44I 977 GTGCAC G^TGCAC AlwNI 1253 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1415 GGGCCC GGGCC^C ApaLI 977 GTGCAC G^TGCAC ApoI 1685 AAATTC [GA]^AATT[CT] AspHI 977 GTGCAC G[AT]GC[AT]^C BamHI 424 GGATCC G^GATCC BanII 1415 GGGCCC G[GA]GC[CT]^C BbrPI 617 CACGTG CAC^GTG BbvCI 227 CCTCAGC CCTCAGC(-5/-2) BciVI 534 GTATCC GTATCC(6/5) BsaWI 500 ACCGGT [AT]^CCGG[AT] BsgI 736 GTGCAG GTGCAG(16/14) BsiEI 429 CGACCG CG[GA][CT]^CG BsiHKAI 977 GTGCAC G[AT]GC[AT]^C BsmBI 1348 CGTCTC CGTCTC(1/5) BsmI 1369 GAATGC GAATGC(1/-1) Bsp1407I 1702 TGTACA T^GTACA BspHI 1560 TCATGA T^CATGA BspLU11I 1699 ACATGT A^CATGT BsrFI 500 ACCGGT [GA]^CCGG[CT] BsrGI 1702 TGTACA T^GTACA BssHII 15 GCGCGC G^CGCGC BstAPI 729 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1688 TTCGAA TT^CGAA Cfr10I 500 ACCGGT [GA]^CCGG[CT] DraI 1662 TTTAAA TTT^AAA DraII 1414 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 1743 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 1414 AGGGCCC [GA]G^G[ACGT]CC[CT] NcoI 994 CCATGG C^CATGG NspI 1699 ACATGT [GA]CATG^[CT] NspV 1688 TTCGAA TT^CGAA PinAI 500 ACCGGT A^CCGGT PmaCI 617 CACGTG CAC^GTG PmlI 617 CACGTG CAC^GTG RcaI 1560 TCATGA T^CATGA ScaI 1775 AGTACT AGT^ACT SfcI 1496 CTACAG C^T[GA][CT]AG SfuI 1688 TTCGAA TT^CGAA SspBI 1702 TGTACA T^GTACA TfiI 1291 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AflIII 1699 ACATGT A^C[GA][CT]GT 1705 ACACGT BmyI 977 GTGCAC G[AGT]GC[ACT]^C 1415 GGGCCC BsaAI 617 CACGTG [CT]AC^GT[GA] 1706 CACGTA BsaI 203 GAGACC GGTCTC(1/5) 222 GAGACC Bsp1286I 977 GTGCAC G[AGT]GC[ACT]^C 1415 GGGCCC DsaI 112 CCACGG C^C[GA][CT]GG 994 CCATGG EarI 653 GAAGAG CTCTTC(1/4) 1362 GAAGAG EcoT14I 626 CCAAGG C^C[AT][AT]GG 994 CCATGG FauI 684 CCCGC CCCGC(4/6) 1067 GCGGG Ksp632I 653 GAAGAG CTCTTC(1/4) 1362 GAAGAG PflMI 365 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1252 CCAGGATCTGG StyI 626 CCAAGG C^C[AT][AT]GG 994 CCATGG Van91I 365 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1252 CCAGGATCTGG XbaI 519 TCTAGA T^CTAGA 774 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BbsI BbvI BcnI BfaI BglI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DrdI EcoNI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII