##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI ApaI AscI AseI
AsnI Asp718I AviII AvrII BbrPI
BcgI BfrI BglII BlnI BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI MvnI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccIII 1998 TCCGGA T^CCGGA
AcsI 396 GAATTT [GA]^AATT[CT]
AflIII 1672 ACATGT A^C[GA][CT]GT
AhdI 210 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1114 GTGCAC G^TGCAC
Aor51HI 102 AGCGCT AGC^GCT
ApaLI 1114 GTGCAC G^TGCAC
ApoI 396 GAATTT [GA]^AATT[CT]
Asp700I 215 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 210 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1027 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 63 CTCGGG C^[CT]CG[GA]G
BalI 2153 TGGCCA TGG^CCA
BamHI 844 GGATCC G^GATCC
BbsI 603 GAAGAC GAAGAC(2/6)
BbvCI 1234 GCTGAGG CCTCAGC(-5/-2)
BclI 817 TGATCA T^GATCA
BglI 130 GCCCCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 123 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 123 GCTCAGC GC^T[ACGT]AGC
BpuAI 603 GAAGAC GAAGAC(2/6)
BsaAI 966 TACGTG [CT]AC^GT[GA]
BsaI 942 GGTCTC GGTCTC(1/5)
BsaWI 1998 TCCGGA [AT]^CCGG[AT]
BseAI 1998 TCCGGA T^CCGGA
BsoBI 63 CTCGGG C^[CT]CG[GA]G
BspEI 1998 TCCGGA T^CCGGA
BspLU11I 1672 ACATGT A^CATGT
BspMI 808 ACCTGC ACCTGC(4/8)
BsrBI 56 GAGCGG CCGCTC(-3/-3)
BsrDI 2192 CATTGC GCAATG(2/0)
BsrFI 47 GCCGGC [GA]^CCGG[CT]
BstXI 1873 CCACAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 123 GCTCAGC GC^T[ACGT]AGC
Cfr10I 47 GCCGGC [GA]^CCGG[CT]
DraI 1403 TTTAAA TTT^AAA
DraIII 1421 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2156 CCATGG C^C[GA][CT]GG
EaeI 2153 TGGCCA [CT]^GGCC[GA]
Eam1105I 210 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2021 CTCTTC CTCTTC(1/4)
Eco47III 102 AGCGCT AGC^GCT
FbaI 817 TGATCA T^GATCA
HgaI 197 GACGC GACGC(5/10)
Ksp632I 2021 CTCTTC CTCTTC(1/4)
MaeIII 2041 GTAAC ^GT[ACGT]AC
MluNI 2153 TGGCCA TGG^CCA
MroI 1998 TCCGGA T^CCGGA
MscI 2153 TGGCCA TGG^CCA
NaeI 47 GCCGGC GCC^GGC
NcoI 2156 CCATGG C^CATGG
NdeI 1520 CATATG CA^TATG
NgoMIV 47 GCCGGC G^CCGGC
PflFI 1027 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1178 GAGTC GAGTC(4/5)
SacI 516 GAGCTC GAGCT^C
StuI 1601 AGGCCT AGG^CCT
Tth111I 1027 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 827 TCTAGA T^CTAGA
XcmI 1609 CCACCTGGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 215 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 114 GGCGCC G[GA]^CG[CT]C
1553 GGCGCC
AocI 2090 CCTAAGG CC^T[ACGT]AGG
2289 CCTAAGG
BbeI 114 GGCGCC GGCGC^C
1553 GGCGCC
BsaHI 114 GGCGCC G[GA]^CG[CT]C
1553 GGCGCC
BsgI 642 CTGCAC GTGCAG(16/14)
1958 GTGCAG
BstAPI 1754 GCACAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1811 GCAGAGGCTGC
BstYI 299 AGATCC [GA]^GATC[CT]
844 GGATCC
Bsu36I 2090 CCTAAGG CC^T[ACGT]AGG
2289 CCTAAGG
Eco81I 2090 CCTAAGG CC^T[ACGT]AGG
2289 CCTAAGG
FauI 69 GCGGG CCCGC(4/6)
1596 GCGGG
Hin1I 114 GGCGCC G[GA]^CG[CT]C
1553 GGCGCC
HphI 184 TCACC GGTGA(8/7)
409 TCACC
KasI 114 GGCGCC G^GCGCC
1553 GGCGCC
MflI 299 AGATCC [GA]^GATC[CT]
844 GGATCC
NarI 114 GGCGCC GG^CGCC
1553 GGCGCC
NspI 482 GCATGT [GA]CATG^[CT]
1672 ACATGT
PpuMI 208 GGGACCC [GA]G^G[AT]CC[CT]
1031 GGGTCCT
SfoI 114 GGCGCC GGC^GCC
1553 GGCGCC
TaqI 292 TCGA T^CGA
1225 TCGA
TfiI 26 GAATC G^A[AT]TC
258 GATTC
TthHB8I 292 TCGA T^CGA
1225 TCGA
XhoII 299 AGATCC [GA]^GATC[CT]
844 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BciVI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MaeII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI Tru9I TseI
Tsp509I TspRI