##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AccII AccIII AflII AflIII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI AspEI AvrII BalI BbrPI
BciVI BclI BfrI BglI BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspI SwaI TfiI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 566 GACGTC GACGT^C
Acc65I 1925 GGTACC G^GTACC
AccI 1721 GTCTAC GT^[AC][GT]AC
AclI 1171 AACGTT AA^CGTT
ApaI 1741 GGGCCC GGGCC^C
Asp700I 101 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1925 GGTACC G^GTACC
AspI 1988 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 660 CCCGGG C^[CT]CG[GA]G
AviII 486 TGCGCA TGC^GCA
BamHI 589 GGATCC G^GATCC
BbeI 405 GGCGCC GGCGC^C
BcgI 739 CGACCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2008 AGATCT A^GATCT
BsaAI 27 TACGTG [CT]AC^GT[GA]
BsmI 1135 GAATGC GAATGC(1/-1)
BsoBI 660 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2120 TGTACA T^GTACA
BspMI 1209 ACCTGC ACCTGC(4/8)
BsrBI 1417 GAGCGG CCGCTC(-3/-3)
BsrDI 1386 CATTGC GCAATG(2/0)
BsrGI 2120 TGTACA T^GTACA
BssSI 959 CTCGTG CACGAG(-5/-1)
BstAPI 478 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1178 GGTGACC G^GT[ACGT]ACC
BstPI 1178 GGTGACC G^GT[ACGT]ACC
DrdI 560 GACTTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1178 GGTGACC G^GT[ACGT]ACC
FauI 1419 GCGGG CCCGC(4/6)
FspI 486 TGCGCA TGC^GCA
HaeII 405 GGCGCC [GA]GCGC^[CT]
HgaI 433 GACGC GACGC(5/10)
HindIII 995 AAGCTT A^AGCTT
KasI 405 GGCGCC G^GCGCC
KpnI 1925 GGTACC GGTAC^C
MfeI 57 CAATTG C^AATTG
MseI 881 TTAA T^TAA
MspA1I 1002 CAGCTG C[AC]G^C[GT]G
MunI 57 CAATTG C^AATTG
NarI 405 GGCGCC GG^CGCC
PflFI 1988 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1687 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1705 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 1171 AACGTT AA^CGTT
PvuII 1002 CAGCTG CAG^CTG
SapI 346 GCTCTTC GCTCTTC(1/4)
SexAI 608 ACCTGGT A^CC[AT]GGT
SfoI 405 GGCGCC GGC^GCC
SmaI 660 CCCGGG CCC^GGG
SphI 1354 GCATGC GCATG^C
SspBI 2120 TGTACA T^GTACA
TaqI 1480 TCGA T^CGA
Tru9I 881 TTAA T^TAA
Tth111I 1988 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1480 TCGA T^CGA
Van91I 1687 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 660 CCCGGG C^CCGGG
XmaI 660 CCCGGG C^CCGGG
XmnI 101 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AciI 502 GCGG CCGC(-3/-1)
1419 GCGG
AcsI 1244 GAATTT [GA]^AATT[CT]
2130 GAATTT
AcyI 405 GGCGCC G[GA]^CG[CT]C
566 GACGTC
Alw44I 515 GTGCAC G^TGCAC
1319 GTGCAC
ApaLI 515 GTGCAC G^TGCAC
1319 GTGCAC
ApoI 1244 GAATTT [GA]^AATT[CT]
2130 GAATTT
BbsI 101 GAAGAC GAAGAC(2/6)
896 GTCTTC
BbvCI 1004 GCTGAGG CCTCAGC(-5/-2)
1333 GCTGAGG
BlpI 536 GCTGAGC GC^T[ACGT]AGC
1635 GCTGAGC
Bpu1102I 536 GCTGAGC GC^T[ACGT]AGC
1635 GCTGAGC
BpuAI 101 GAAGAC GAAGAC(2/6)
896 GTCTTC
BsaHI 405 GGCGCC G[GA]^CG[CT]C
566 GACGTC
CelII 536 GCTGAGC GC^T[ACGT]AGC
1635 GCTGAGC
DraIII 607 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1555 CACACTGTG
Hin1I 405 GGCGCC G[GA]^CG[CT]C
566 GACGTC
HinfI 1950 GAGTC G^A[ACGT]TC
1992 GAGTC
NspI 239 GCATGT [GA]CATG^[CT]
1354 GCATGC
PleI 1950 GAGTC GAGTC(4/5)
1992 GAGTC
StuI 1829 AGGCCT AGG^CCT
1961 AGGCCT
XcmI 280 CCAGGCTCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1207 CCACCTGCTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I Tsp509I
TspRI XhoII