##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AccI AccIII AclI AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp700I AspI AviII AvrII BamHI
BbeI BlnI BsaBI BsaWI BseAI
BsgI BsiWI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KasI MamI MfeI MluI
MroI MunI NarI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2241 GGTACC G^GTACC
AflII 2649 CTTAAG C^TTAAG
AflIII 1145 ACGTGT A^C[GA][CT]GT
AhdI 962 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 698 AGCGCT AGC^GCT
Asp718I 2241 GGTACC G^GTACC
AspEI 962 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1144 CACGTG CAC^GTG
BcgI 20 GCAGCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 558 GGATAC GTATCC(6/5)
BclI 1873 TGATCA T^GATCA
BfrI 2649 CTTAAG C^TTAAG
BglII 2080 AGATCT A^GATCT
BlpI 2012 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2012 GCTGAGC GC^T[ACGT]AGC
BsaAI 1144 CACGTG [CT]AC^GT[GA]
BsaI 1998 GAGACC GGTCTC(1/5)
BsiEI 3 CGGCCG CG[GA][CT]^CG
BsmBI 2613 GAGACG CGTCTC(1/5)
BsmI 893 GCATTC GAATGC(1/-1)
BspMI 2244 ACCTGC ACCTGC(4/8)
BsrBI 687 GAGCGG CCGCTC(-3/-3)
BstEII 1211 GGTGACC G^GT[ACGT]ACC
BstPI 1211 GGTGACC G^GT[ACGT]ACC
CelII 2012 GCTGAGC GC^T[ACGT]AGC
DraI 2450 TTTAAA TTT^AAA
DrdI 1784 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3 CGGCCG C^GGCCG
Eam1105I 962 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3 CGGCCG C^GGCCG
Eco47III 698 AGCGCT AGC^GCT
Eco52I 3 CGGCCG C^GGCCG
EcoO65I 1211 GGTGACC G^GT[ACGT]ACC
EcoRI 2138 GAATTC G^AATTC
FbaI 1873 TGATCA T^GATCA
HindIII 1276 AAGCTT A^AGCTT
KpnI 2241 GGTACC GGTAC^C
KspI 2185 CCGCGG CCGC^GG
NcoI 2605 CCATGG C^CATGG
NdeI 2659 CATATG CA^TATG
NheI 1002 GCTAGC G^CTAGC
PmaCI 1144 CACGTG CAC^GTG
PmlI 1144 CACGTG CAC^GTG
SacII 2185 CCGCGG CCGC^GG
SexAI 1085 ACCAGGT A^CC[AT]GGT
SfiI 49 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2552 GCATGC GCATG^C
StuI 1904 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 298 GACGTC GACGT^C
962 GACGTC
AccII 38 CGCG CG^CG
2186 CGCG
AlwI 408 GATCC GGATC(4/5)
939 GATCC
AocI 1602 CCTGAGG CC^T[ACGT]AGG
2109 CCTAAGG
BalI 722 TGGCCA TGG^CCA
2421 TGGCCA
BbsI 824 GAAGAC GAAGAC(2/6)
1803 GAAGAC
BbvCI 792 CCTCAGC CCTCAGC(-5/-2)
1668 CCTCAGC
BglI 5 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
50 GCCCGCCTGGC
BpmI 1198 CTCCAG CTGGAG(16/14)
1137 CTGGAG
BpuAI 824 GAAGAC GAAGAC(2/6)
1803 GAAGAC
Bsp1407I 836 TGTACA T^GTACA
1825 TGTACA
BsrDI 1483 CATTGC GCAATG(2/0)
1722 CATTGC
BsrGI 836 TGTACA T^GTACA
1825 TGTACA
BstUI 38 CGCG CG^CG
2186 CGCG
BstXI 626 CCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1778 CCATGTGACTGG
Bsu36I 1602 CCTGAGG CC^T[ACGT]AGG
2109 CCTAAGG
DraII 137 AGGACCT [GA]G^G[ACGT]CC[CT]
1956 GGGTCCC
DraIII 376 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
491 CACACAGTG
DsaI 2185 CCGCGG C^C[GA][CT]GG
2605 CCATGG
Eco81I 1602 CCTGAGG CC^T[ACGT]AGG
2109 CCTAAGG
EcoNI 2105 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2245 CCTGCAGTAGG
EcoO109I 137 AGGACCT [GA]G^G[ACGT]CC[CT]
1956 GGGTCCC
MluNI 722 TGGCCA TGG^CCA
2421 TGGCCA
MscI 722 TGGCCA TGG^CCA
2421 TGGCCA
MvnI 38 CGCG CG^CG
2186 CGCG
NaeI 427 GCCGGC GCC^GGC
889 GCCGGC
NgoMIV 427 GCCGGC G^CCGGC
889 GCCGGC
PpuMI 137 AGGACCT [GA]G^G[AT]CC[CT]
1956 GGGTCCC
PshAI 298 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1173 GACCAGGGTC
SacI 217 GAGCTC GAGCT^C
253 GAGCTC
SmaI 1346 CCCGGG CCC^GGG
1507 CCCGGG
SmlI 2168 CTCAAG C^T[CT][GA]AG
2649 CTTAAG
SspBI 836 TGTACA T^GTACA
1825 TGTACA
XmaCI 1346 CCCGGG C^CCGGG
1507 CCCGGG
XmaI 1346 CCCGGG C^CCGGG
1507 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII