################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AccI AccIII AclI AgeI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbeI BlnI BsaBI BsaWI BseAI BsgI BsiWI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI MamI MfeI MluI MroI MunI NarI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 2241 GGTACC G^GTACC AflII 2649 CTTAAG C^TTAAG AflIII 1145 ACGTGT A^C[GA][CT]GT AhdI 962 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 698 AGCGCT AGC^GCT Asp718I 2241 GGTACC G^GTACC AspEI 962 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1144 CACGTG CAC^GTG BcgI 20 GCAGCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 558 GGATAC GTATCC(6/5) BclI 1873 TGATCA T^GATCA BfrI 2649 CTTAAG C^TTAAG BglII 2080 AGATCT A^GATCT BlpI 2012 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2012 GCTGAGC GC^T[ACGT]AGC BsaAI 1144 CACGTG [CT]AC^GT[GA] BsaI 1998 GAGACC GGTCTC(1/5) BsiEI 3 CGGCCG CG[GA][CT]^CG BsmBI 2613 GAGACG CGTCTC(1/5) BsmI 893 GCATTC GAATGC(1/-1) BspMI 2244 ACCTGC ACCTGC(4/8) BsrBI 687 GAGCGG CCGCTC(-3/-3) BstEII 1211 GGTGACC G^GT[ACGT]ACC BstPI 1211 GGTGACC G^GT[ACGT]ACC CelII 2012 GCTGAGC GC^T[ACGT]AGC DraI 2450 TTTAAA TTT^AAA DrdI 1784 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3 CGGCCG C^GGCCG Eam1105I 962 GACGTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3 CGGCCG C^GGCCG Eco47III 698 AGCGCT AGC^GCT Eco52I 3 CGGCCG C^GGCCG EcoO65I 1211 GGTGACC G^GT[ACGT]ACC EcoRI 2138 GAATTC G^AATTC FbaI 1873 TGATCA T^GATCA HindIII 1276 AAGCTT A^AGCTT KpnI 2241 GGTACC GGTAC^C KspI 2185 CCGCGG CCGC^GG NcoI 2605 CCATGG C^CATGG NdeI 2659 CATATG CA^TATG NheI 1002 GCTAGC G^CTAGC PmaCI 1144 CACGTG CAC^GTG PmlI 1144 CACGTG CAC^GTG SacII 2185 CCGCGG CCGC^GG SexAI 1085 ACCAGGT A^CC[AT]GGT SfiI 49 GGCCCGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 2552 GCATGC GCATG^C StuI 1904 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AatII 298 GACGTC GACGT^C 962 GACGTC AccII 38 CGCG CG^CG 2186 CGCG AlwI 408 GATCC GGATC(4/5) 939 GATCC AocI 1602 CCTGAGG CC^T[ACGT]AGG 2109 CCTAAGG BalI 722 TGGCCA TGG^CCA 2421 TGGCCA BbsI 824 GAAGAC GAAGAC(2/6) 1803 GAAGAC BbvCI 792 CCTCAGC CCTCAGC(-5/-2) 1668 CCTCAGC BglI 5 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 50 GCCCGCCTGGC BpmI 1198 CTCCAG CTGGAG(16/14) 1137 CTGGAG BpuAI 824 GAAGAC GAAGAC(2/6) 1803 GAAGAC Bsp1407I 836 TGTACA T^GTACA 1825 TGTACA BsrDI 1483 CATTGC GCAATG(2/0) 1722 CATTGC BsrGI 836 TGTACA T^GTACA 1825 TGTACA BstUI 38 CGCG CG^CG 2186 CGCG BstXI 626 CCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1778 CCATGTGACTGG Bsu36I 1602 CCTGAGG CC^T[ACGT]AGG 2109 CCTAAGG DraII 137 AGGACCT [GA]G^G[ACGT]CC[CT] 1956 GGGTCCC DraIII 376 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG 491 CACACAGTG DsaI 2185 CCGCGG C^C[GA][CT]GG 2605 CCATGG Eco81I 1602 CCTGAGG CC^T[ACGT]AGG 2109 CCTAAGG EcoNI 2105 CCTTCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2245 CCTGCAGTAGG EcoO109I 137 AGGACCT [GA]G^G[ACGT]CC[CT] 1956 GGGTCCC MluNI 722 TGGCCA TGG^CCA 2421 TGGCCA MscI 722 TGGCCA TGG^CCA 2421 TGGCCA MvnI 38 CGCG CG^CG 2186 CGCG NaeI 427 GCCGGC GCC^GGC 889 GCCGGC NgoMIV 427 GCCGGC G^CCGGC 889 GCCGGC PpuMI 137 AGGACCT [GA]G^G[AT]CC[CT] 1956 GGGTCCC PshAI 298 GACGTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1173 GACCAGGGTC SacI 217 GAGCTC GAGCT^C 253 GAGCTC SmaI 1346 CCCGGG CCC^GGG 1507 CCCGGG SmlI 2168 CTCAAG C^T[CT][GA]AG 2649 CTTAAG SspBI 836 TGTACA T^GTACA 1825 TGTACA XmaCI 1346 CCCGGG C^CCGGG 1507 CCCGGG XmaI 1346 CCCGGG C^CCGGG 1507 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII