##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflIII      
AgeI        AhdI        AocI        ApaI        AscI        
AspEI       AviII       AvrII       BalI        BbeI        
BbrPI       BbvCI       BcnI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      BssSI       BstBI       BstEII      
BstPI       BstXI       Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       FseI        FspI        HaeIII      
HapII       HgaI        Hin1I       HpaII       KasI        
MfeI        MluI        MluNI       MroI        MscI        
MspI        MunI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

Acc65I        573  GGTACC               G^GTACC
AccI         1494  GTATAC               GT^[AC][GT]AC
AccII        1351  CGCG                 CG^CG
AflII          47  CTTAAG               C^TTAAG
Alw44I       1163  GTGCAC               G^TGCAC
AlwNI        2527  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       159  AGCGCT               AGC^GCT
ApaLI        1163  GTGCAC               G^TGCAC
Asp718I       573  GGTACC               G^GTACC
AspI         2015  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1346  GGATCC               G^GATCC
BanII        1939  GAGCCC               G[GA]GC[CT]^C
BcgI         1448  CGATTTCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3196  GTATCC               GTATCC(6/5)
BclI         1013  TGATCA               T^GATCA
BfrI           47  CTTAAG               C^TTAAG
BglII        1757  AGATCT               A^GATCT
BpmI         1651  CTGGAG               CTGGAG(16/14)
BsaBI        3018  GATTAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1047  GAGACC               GGTCTC(1/5)
BsgI          754  GTGCAG               GTGCAG(16/14)
BsmBI        1114  GAGACG               CGTCTC(1/5)
BsmFI        1308  GTCCC                GGGAC(10/14)
BsmI          193  GCATTC               GAATGC(1/-1)
BspMI        1747  GCAGGT               ACCTGC(4/8)
BsrDI        2490  GCAATG               GCAATG(2/0)
Bst1107I     1494  GTATAC               GTA^TAC
BstAPI        157  GCAGCGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1351  CGCG                 CG^CG
BstZ17I      1494  GTATAC               GTA^TAC
DraI         3182  TTTAAA               TTT^AAA
DraII        1627  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI         1350  CCGCGG               C^C[GA][CT]GG
Eco47III      159  AGCGCT               AGC^GCT
EcoNI         315  CCTTGTCTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1627  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV        2322  GATATC               GAT^ATC
EcoT22I      1131  ATGCAT               ATGCA^T
FauI         1860  GCGGG                CCCGC(4/6)
FbaI         1013  TGATCA               T^GATCA
HpaI          212  GTTAAC               GTT^AAC
KpnI          573  GGTACC               GGTAC^C
KspI         1350  CCGCGG               CCGC^GG
MaeII        1383  ACGT                 A^CGT
MamI         3018  GATTAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1351  CGCG                 CG^CG
NdeI         1192  CATATG               CA^TATG
NsiI         1131  ATGCAT               ATGCA^T
PaeR7I       1926  CTCGAG               C^TCGAG
PflFI        2015  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         878  CCAACCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1627  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         765  CAGCTG               CAG^CTG
SacII        1350  CCGCGG               CCGC^GG
SexAI         648  ACCAGGT              A^CC[AT]GGT
Tth111I      2015  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        878  CCAACCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1926  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AseI          542  ATTAAT               AT^TAAT
             3011  ATTAAT              
AsnI          542  ATTAAT               AT^TAAT
             3011  ATTAAT              
Asp700I       735  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2031  GAACTTGTTC          
AspHI         657  GAGCAC               G[AT]GC[AT]^C
             1163  GTGCAC              
AvaI           94  CCCGAG               C^[CT]CG[GA]G
             1926  CTCGAG              
AvaII        1307  GGTCC                G^G[AT]CC
             1628  GGACC               
BanI          243  GGTGCC               G^G[CT][GA]CC
              573  GGTACC              
BseRI        1588  CTCCTC               GAGGAG(10/8)
             2460  GAGGAG              
BsiHKAI       657  GAGCAC               G[AT]GC[AT]^C
             1163  GTGCAC              
BsoBI          94  CCCGAG               C^[CT]CG[GA]G
             1926  CTCGAG              
Cfr13I       1307  GGTCC                G^G[ACGT]CC
             1628  GGACC               
EcoT14I       724  CCAAGG               C^C[AT][AT]GG
              883  CCTTGG              
HaeII         159  AGCGCT               [GA]GCGC^[CT]
             2135  GGCGCT              
HincII        212  GTTAAC               GT[CT]^[GA]AC
              597  GTTGAC              
HindII        212  GTTAAC               GT[CT]^[GA]AC
              597  GTTGAC              
HindIII       416  AAGCTT               A^AGCTT
             1325  AAGCTT              
MspA1I        765  CAGCTG               C[AC]G^C[GT]G
             1350  CCGCGG              
NspI         1133  GCATGT               [GA]CATG^[CT]
             2843  ACATGC              
PshBI         542  ATTAAT               AT^TAAT
             3011  ATTAAT              
PstI         1644  CTGCAG               CTGCA^G
             1996  CTGCAG              
SapI         1893  GAAGAGC              GCTCTTC(1/4)
             2184  GAAGAGC             
Sau96I       1307  GGTCC                G^G[ACGT]CC
             1628  GGACC               
ScaI          589  AGTACT               AGT^ACT
              683  AGTACT              
SfaNI        1517  GCATC                GCATC(5/9)
             1958  GCATC               
StyI          724  CCAAGG               C^C[AT][AT]GG
              883  CCTTGG              
XcmI          878  CCAACCCTTGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2498  CCACAGTTTAAGTGG     
XmnI          735  GAATTACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2031  GAACTTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbsI        BbvI        BfaI        BmyI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        DdeI        DpnII       
EarI        EcoRII      Fnu4HI      FokI        HhaI        
HinP1I      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        RsaI        
Sau3AI      ScrFI       SfcI        SmlI        SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII