################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AccIII AclI AcyI AflIII AgeI AhdI AocI ApaI AscI AspEI AviII AvrII BalI BbeI BbrPI BbvCI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI FseI FspI HaeIII HapII HgaI Hin1I HpaII KasI MfeI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 573 GGTACC G^GTACC AccI 1494 GTATAC GT^[AC][GT]AC AccII 1351 CGCG CG^CG AflII 47 CTTAAG C^TTAAG Alw44I 1163 GTGCAC G^TGCAC AlwNI 2527 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 159 AGCGCT AGC^GCT ApaLI 1163 GTGCAC G^TGCAC Asp718I 573 GGTACC G^GTACC AspI 2015 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1346 GGATCC G^GATCC BanII 1939 GAGCCC G[GA]GC[CT]^C BcgI 1448 CGATTTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3196 GTATCC GTATCC(6/5) BclI 1013 TGATCA T^GATCA BfrI 47 CTTAAG C^TTAAG BglII 1757 AGATCT A^GATCT BpmI 1651 CTGGAG CTGGAG(16/14) BsaBI 3018 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1047 GAGACC GGTCTC(1/5) BsgI 754 GTGCAG GTGCAG(16/14) BsmBI 1114 GAGACG CGTCTC(1/5) BsmFI 1308 GTCCC GGGAC(10/14) BsmI 193 GCATTC GAATGC(1/-1) BspMI 1747 GCAGGT ACCTGC(4/8) BsrDI 2490 GCAATG GCAATG(2/0) Bst1107I 1494 GTATAC GTA^TAC BstAPI 157 GCAGCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1351 CGCG CG^CG BstZ17I 1494 GTATAC GTA^TAC DraI 3182 TTTAAA TTT^AAA DraII 1627 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 1350 CCGCGG C^C[GA][CT]GG Eco47III 159 AGCGCT AGC^GCT EcoNI 315 CCTTGTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1627 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 2322 GATATC GAT^ATC EcoT22I 1131 ATGCAT ATGCA^T FauI 1860 GCGGG CCCGC(4/6) FbaI 1013 TGATCA T^GATCA HpaI 212 GTTAAC GTT^AAC KpnI 573 GGTACC GGTAC^C KspI 1350 CCGCGG CCGC^GG MaeII 1383 ACGT A^CGT MamI 3018 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 1351 CGCG CG^CG NdeI 1192 CATATG CA^TATG NsiI 1131 ATGCAT ATGCA^T PaeR7I 1926 CTCGAG C^TCGAG PflFI 2015 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 878 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1627 AGGACCT [GA]G^G[AT]CC[CT] PvuII 765 CAGCTG CAG^CTG SacII 1350 CCGCGG CCGC^GG SexAI 648 ACCAGGT A^CC[AT]GGT Tth111I 2015 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 878 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1926 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AseI 542 ATTAAT AT^TAAT 3011 ATTAAT AsnI 542 ATTAAT AT^TAAT 3011 ATTAAT Asp700I 735 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2031 GAACTTGTTC AspHI 657 GAGCAC G[AT]GC[AT]^C 1163 GTGCAC AvaI 94 CCCGAG C^[CT]CG[GA]G 1926 CTCGAG AvaII 1307 GGTCC G^G[AT]CC 1628 GGACC BanI 243 GGTGCC G^G[CT][GA]CC 573 GGTACC BseRI 1588 CTCCTC GAGGAG(10/8) 2460 GAGGAG BsiHKAI 657 GAGCAC G[AT]GC[AT]^C 1163 GTGCAC BsoBI 94 CCCGAG C^[CT]CG[GA]G 1926 CTCGAG Cfr13I 1307 GGTCC G^G[ACGT]CC 1628 GGACC EcoT14I 724 CCAAGG C^C[AT][AT]GG 883 CCTTGG HaeII 159 AGCGCT [GA]GCGC^[CT] 2135 GGCGCT HincII 212 GTTAAC GT[CT]^[GA]AC 597 GTTGAC HindII 212 GTTAAC GT[CT]^[GA]AC 597 GTTGAC HindIII 416 AAGCTT A^AGCTT 1325 AAGCTT MspA1I 765 CAGCTG C[AC]G^C[GT]G 1350 CCGCGG NspI 1133 GCATGT [GA]CATG^[CT] 2843 ACATGC PshBI 542 ATTAAT AT^TAAT 3011 ATTAAT PstI 1644 CTGCAG CTGCA^G 1996 CTGCAG SapI 1893 GAAGAGC GCTCTTC(1/4) 2184 GAAGAGC Sau96I 1307 GGTCC G^G[ACGT]CC 1628 GGACC ScaI 589 AGTACT AGT^ACT 683 AGTACT SfaNI 1517 GCATC GCATC(5/9) 1958 GCATC StyI 724 CCAAGG C^C[AT][AT]GG 883 CCTTGG XcmI 878 CCAACCCTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2498 CCACAGTTTAAGTGG XmnI 735 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2031 GAACTTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbsI BbvI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI DdeI DpnII EarI EcoRII Fnu4HI FokI HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII