##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI AocI ApaI AscI
AspEI AviII AvrII BalI BbeI
BbrPI BbvCI BcnI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstXI Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI FseI FspI HaeIII
HapII HgaI Hin1I HpaII KasI
MfeI MluI MluNI MroI MscI
MspI MunI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 573 GGTACC G^GTACC
AccI 1494 GTATAC GT^[AC][GT]AC
AccII 1351 CGCG CG^CG
AflII 47 CTTAAG C^TTAAG
Alw44I 1163 GTGCAC G^TGCAC
AlwNI 2527 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 159 AGCGCT AGC^GCT
ApaLI 1163 GTGCAC G^TGCAC
Asp718I 573 GGTACC G^GTACC
AspI 2015 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1346 GGATCC G^GATCC
BanII 1939 GAGCCC G[GA]GC[CT]^C
BcgI 1448 CGATTTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3196 GTATCC GTATCC(6/5)
BclI 1013 TGATCA T^GATCA
BfrI 47 CTTAAG C^TTAAG
BglII 1757 AGATCT A^GATCT
BpmI 1651 CTGGAG CTGGAG(16/14)
BsaBI 3018 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1047 GAGACC GGTCTC(1/5)
BsgI 754 GTGCAG GTGCAG(16/14)
BsmBI 1114 GAGACG CGTCTC(1/5)
BsmFI 1308 GTCCC GGGAC(10/14)
BsmI 193 GCATTC GAATGC(1/-1)
BspMI 1747 GCAGGT ACCTGC(4/8)
BsrDI 2490 GCAATG GCAATG(2/0)
Bst1107I 1494 GTATAC GTA^TAC
BstAPI 157 GCAGCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1351 CGCG CG^CG
BstZ17I 1494 GTATAC GTA^TAC
DraI 3182 TTTAAA TTT^AAA
DraII 1627 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1350 CCGCGG C^C[GA][CT]GG
Eco47III 159 AGCGCT AGC^GCT
EcoNI 315 CCTTGTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1627 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 2322 GATATC GAT^ATC
EcoT22I 1131 ATGCAT ATGCA^T
FauI 1860 GCGGG CCCGC(4/6)
FbaI 1013 TGATCA T^GATCA
HpaI 212 GTTAAC GTT^AAC
KpnI 573 GGTACC GGTAC^C
KspI 1350 CCGCGG CCGC^GG
MaeII 1383 ACGT A^CGT
MamI 3018 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1351 CGCG CG^CG
NdeI 1192 CATATG CA^TATG
NsiI 1131 ATGCAT ATGCA^T
PaeR7I 1926 CTCGAG C^TCGAG
PflFI 2015 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 878 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1627 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 765 CAGCTG CAG^CTG
SacII 1350 CCGCGG CCGC^GG
SexAI 648 ACCAGGT A^CC[AT]GGT
Tth111I 2015 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 878 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1926 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 542 ATTAAT AT^TAAT
3011 ATTAAT
AsnI 542 ATTAAT AT^TAAT
3011 ATTAAT
Asp700I 735 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2031 GAACTTGTTC
AspHI 657 GAGCAC G[AT]GC[AT]^C
1163 GTGCAC
AvaI 94 CCCGAG C^[CT]CG[GA]G
1926 CTCGAG
AvaII 1307 GGTCC G^G[AT]CC
1628 GGACC
BanI 243 GGTGCC G^G[CT][GA]CC
573 GGTACC
BseRI 1588 CTCCTC GAGGAG(10/8)
2460 GAGGAG
BsiHKAI 657 GAGCAC G[AT]GC[AT]^C
1163 GTGCAC
BsoBI 94 CCCGAG C^[CT]CG[GA]G
1926 CTCGAG
Cfr13I 1307 GGTCC G^G[ACGT]CC
1628 GGACC
EcoT14I 724 CCAAGG C^C[AT][AT]GG
883 CCTTGG
HaeII 159 AGCGCT [GA]GCGC^[CT]
2135 GGCGCT
HincII 212 GTTAAC GT[CT]^[GA]AC
597 GTTGAC
HindII 212 GTTAAC GT[CT]^[GA]AC
597 GTTGAC
HindIII 416 AAGCTT A^AGCTT
1325 AAGCTT
MspA1I 765 CAGCTG C[AC]G^C[GT]G
1350 CCGCGG
NspI 1133 GCATGT [GA]CATG^[CT]
2843 ACATGC
PshBI 542 ATTAAT AT^TAAT
3011 ATTAAT
PstI 1644 CTGCAG CTGCA^G
1996 CTGCAG
SapI 1893 GAAGAGC GCTCTTC(1/4)
2184 GAAGAGC
Sau96I 1307 GGTCC G^G[ACGT]CC
1628 GGACC
ScaI 589 AGTACT AGT^ACT
683 AGTACT
SfaNI 1517 GCATC GCATC(5/9)
1958 GCATC
StyI 724 CCAAGG C^C[AT][AT]GG
883 CCTTGG
XcmI 878 CCAACCCTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2498 CCACAGTTTAAGTGG
XmnI 735 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2031 GAACTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BfaI BmyI
BpuAI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI DdeI DpnII
EarI EcoRII Fnu4HI FokI HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI SfcI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII