##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
BamHI BbeI BbvCI BciVI BglI
BglII BlpI Bpu1102I BsaHI BsaI
BseRI BsiEI BsiWI BsmBI BsmI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoRI
EcoRV FauI FseI FspI HgaI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflMI PinAI PmeI
PshAI PshBI PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 2724 GTAGAC GT^[AC][GT]AC
AccIII 24 TCCGGA T^CCGGA
AciI 95 GCGG CCGC(-3/-1)
AclI 919 AACGTT AA^CGTT
AflIII 2396 ACATGT A^C[GA][CT]GT
Aor51HI 1727 AGCGCT AGC^GCT
AspI 122 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1047 CTCGAG C^[CT]CG[GA]G
AvrII 2337 CCTAGG C^CTAGG
BalI 1361 TGGCCA TGG^CCA
BbrPI 2003 CACGTG CAC^GTG
BclI 2007 TGATCA T^GATCA
BlnI 2337 CCTAGG C^CTAGG
BsaAI 2003 CACGTG [CT]AC^GT[GA]
BsaWI 24 TCCGGA [AT]^CCGG[AT]
BseAI 24 TCCGGA T^CCGGA
BsoBI 1047 CTCGAG C^[CT]CG[GA]G
BspEI 24 TCCGGA T^CCGGA
BspLU11I 2396 ACATGT A^CATGT
BssSI 1405 CACGAG CACGAG(-5/-1)
BstEII 2406 GGTAACC G^GT[ACGT]ACC
BstPI 2406 GGTAACC G^GT[ACGT]ACC
DraI 2383 TTTAAA TTT^AAA
DraIII 2098 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1361 TGGCCA [CT]^GGCC[GA]
Eco47III 1727 AGCGCT AGC^GCT
EcoNI 741 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2406 GGTAACC G^GT[ACGT]ACC
EcoT22I 276 ATGCAT ATGCA^T
FbaI 2007 TGATCA T^GATCA
HindIII 1194 AAGCTT A^AGCTT
MluNI 1361 TGGCCA TGG^CCA
MroI 24 TCCGGA T^CCGGA
MscI 1361 TGGCCA TGG^CCA
NdeI 1428 CATATG CA^TATG
NsiI 276 ATGCAT ATGCA^T
PaeR7I 1047 CTCGAG C^TCGAG
PflFI 122 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2003 CACGTG CAC^GTG
PmlI 2003 CACGTG CAC^GTG
Psp1406I 919 AACGTT AA^CGTT
PstI 83 CTGCAG CTGCA^G
SacI 59 GAGCTC GAGCT^C
SapI 553 GAAGAGC GCTCTTC(1/4)
SexAI 2809 ACCTGGT A^CC[AT]GGT
StuI 2334 AGGCCT AGG^CCT
Tth111I 122 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2615 TCTAGA T^CTAGA
XcmI 102 CCAACTTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1047 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 2079 CTTAAG C^TTAAG
2975 CTTAAG
AlwNI 1123 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1448 CAGAGTCTG
ApaI 1042 GGGCCC GGGCC^C
1152 GGGCCC
Asp700I 1596 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1993 GAAGACATTC
AspHI 59 GAGCTC G[AT]GC[AT]^C
2814 GTGCTC
BanI 630 GGCACC G^G[CT][GA]CC
1325 GGCACC
BcgI 1407 CGAGAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2058 GCAGATTCATCG
BfrI 2079 CTTAAG C^TTAAG
2975 CTTAAG
BsaBI 2188 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2648 GATTATTATC
BsgI 1145 GTGCAG GTGCAG(16/14)
1702 GTGCAG
BsiHKAI 59 GAGCTC G[AT]GC[AT]^C
2814 GTGCTC
BsmFI 881 GGGAC GGGAC(10/14)
1837 GTCCC
BspDI 2240 ATCGAT AT^CGAT
2995 ATCGAT
BstYI 1978 AGATCC [GA]^GATC[CT]
2437 GGATCT
ClaI 2240 ATCGAT AT^CGAT
2995 ATCGAT
HaeII 1727 AGCGCT [GA]GCGC^[CT]
2304 GGCGCT
HincII 164 GTCAAC GT[CT]^[GA]AC
2226 GTCAAC
HindII 164 GTCAAC GT[CT]^[GA]AC
2226 GTCAAC
MamI 2188 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2648 GATTATTATC
MflI 1978 AGATCC [GA]^GATC[CT]
2437 GGATCT
NspI 2069 GCATGT [GA]CATG^[CT]
2396 ACATGT
PleI 1450 GAGTC GAGTC(4/5)
2044 GAGTC
PpuMI 653 AGGTCCT [GA]G^G[AT]CC[CT]
1835 AGGTCCC
ScaI 673 AGTACT AGT^ACT
1577 AGTACT
XhoII 1978 AGATCC [GA]^GATC[CT]
2437 GGATCT
XmnI 1596 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1993 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI Bsp1286I Bsp1407I
BspHI BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I