################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccII AcyI AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AviII BamHI BbeI BbvCI BciVI BglI BglII BlpI Bpu1102I BsaHI BsaI BseRI BsiEI BsiWI BsmBI BsmI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV FauI FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflMI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Van91I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 2724 GTAGAC GT^[AC][GT]AC AccIII 24 TCCGGA T^CCGGA AciI 95 GCGG CCGC(-3/-1) AclI 919 AACGTT AA^CGTT AflIII 2396 ACATGT A^C[GA][CT]GT Aor51HI 1727 AGCGCT AGC^GCT AspI 122 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1047 CTCGAG C^[CT]CG[GA]G AvrII 2337 CCTAGG C^CTAGG BalI 1361 TGGCCA TGG^CCA BbrPI 2003 CACGTG CAC^GTG BclI 2007 TGATCA T^GATCA BlnI 2337 CCTAGG C^CTAGG BsaAI 2003 CACGTG [CT]AC^GT[GA] BsaWI 24 TCCGGA [AT]^CCGG[AT] BseAI 24 TCCGGA T^CCGGA BsoBI 1047 CTCGAG C^[CT]CG[GA]G BspEI 24 TCCGGA T^CCGGA BspLU11I 2396 ACATGT A^CATGT BssSI 1405 CACGAG CACGAG(-5/-1) BstEII 2406 GGTAACC G^GT[ACGT]ACC BstPI 2406 GGTAACC G^GT[ACGT]ACC DraI 2383 TTTAAA TTT^AAA DraIII 2098 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1361 TGGCCA [CT]^GGCC[GA] Eco47III 1727 AGCGCT AGC^GCT EcoNI 741 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2406 GGTAACC G^GT[ACGT]ACC EcoT22I 276 ATGCAT ATGCA^T FbaI 2007 TGATCA T^GATCA HindIII 1194 AAGCTT A^AGCTT MluNI 1361 TGGCCA TGG^CCA MroI 24 TCCGGA T^CCGGA MscI 1361 TGGCCA TGG^CCA NdeI 1428 CATATG CA^TATG NsiI 276 ATGCAT ATGCA^T PaeR7I 1047 CTCGAG C^TCGAG PflFI 122 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2003 CACGTG CAC^GTG PmlI 2003 CACGTG CAC^GTG Psp1406I 919 AACGTT AA^CGTT PstI 83 CTGCAG CTGCA^G SacI 59 GAGCTC GAGCT^C SapI 553 GAAGAGC GCTCTTC(1/4) SexAI 2809 ACCTGGT A^CC[AT]GGT StuI 2334 AGGCCT AGG^CCT Tth111I 122 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2615 TCTAGA T^CTAGA XcmI 102 CCAACTTCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1047 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflII 2079 CTTAAG C^TTAAG 2975 CTTAAG AlwNI 1123 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1448 CAGAGTCTG ApaI 1042 GGGCCC GGGCC^C 1152 GGGCCC Asp700I 1596 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1993 GAAGACATTC AspHI 59 GAGCTC G[AT]GC[AT]^C 2814 GTGCTC BanI 630 GGCACC G^G[CT][GA]CC 1325 GGCACC BcgI 1407 CGAGAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2058 GCAGATTCATCG BfrI 2079 CTTAAG C^TTAAG 2975 CTTAAG BsaBI 2188 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2648 GATTATTATC BsgI 1145 GTGCAG GTGCAG(16/14) 1702 GTGCAG BsiHKAI 59 GAGCTC G[AT]GC[AT]^C 2814 GTGCTC BsmFI 881 GGGAC GGGAC(10/14) 1837 GTCCC BspDI 2240 ATCGAT AT^CGAT 2995 ATCGAT BstYI 1978 AGATCC [GA]^GATC[CT] 2437 GGATCT ClaI 2240 ATCGAT AT^CGAT 2995 ATCGAT HaeII 1727 AGCGCT [GA]GCGC^[CT] 2304 GGCGCT HincII 164 GTCAAC GT[CT]^[GA]AC 2226 GTCAAC HindII 164 GTCAAC GT[CT]^[GA]AC 2226 GTCAAC MamI 2188 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2648 GATTATTATC MflI 1978 AGATCC [GA]^GATC[CT] 2437 GGATCT NspI 2069 GCATGT [GA]CATG^[CT] 2396 ACATGT PleI 1450 GAGTC GAGTC(4/5) 2044 GAGTC PpuMI 653 AGGTCCT [GA]G^G[AT]CC[CT] 1835 AGGTCCC ScaI 673 AGTACT AGT^ACT 1577 AGTACT XhoII 1978 AGATCC [GA]^GATC[CT] 2437 GGATCT XmnI 1596 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1993 GAAGACATTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I Bsp1407I BspHI BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I