################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII AvrII BalI BamHI BbrPI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BseAI BsiEI BsiHKAI BsiWI BspDI BspEI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I CelII ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FauI FseI FspI HindIII KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflMI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 764 GGCGCC G[GA]^CG[CT]C AgeI 856 ACCGGT A^CCGGT AlwI 2482 GATCC GGATC(4/5) Asp700I 397 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 829 CTCGAG C^[CT]CG[GA]G BanII 1067 GGGCTC G[GA]GC[CT]^C BbeI 764 GGCGCC GGCGC^C BbvCI 586 GCTGAGG CCTCAGC(-5/-2) BglII 163 AGATCT A^GATCT BmyI 1067 GGGCTC G[AGT]GC[ACT]^C BsaAI 278 TACGTG [CT]AC^GT[GA] BsaBI 1329 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 764 GGCGCC G[GA]^CG[CT]C BsaI 996 GAGACC GGTCTC(1/5) BsaWI 856 ACCGGT [AT]^CCGG[AT] BsgI 2270 GTGCAG GTGCAG(16/14) BsmFI 1400 GGGAC GGGAC(10/14) BsoBI 829 CTCGAG C^[CT]CG[GA]G Bsp1286I 1067 GGGCTC G[AGT]GC[ACT]^C BspMI 955 ACCTGC ACCTGC(4/8) BsrFI 856 ACCGGT [GA]^CCGG[CT] Cfr10I 856 ACCGGT [GA]^CCGG[CT] EcoRV 2602 GATATC GAT^ATC HapII 857 CCGG C^CGG HgaI 446 GACGC GACGC(5/10) Hin1I 764 GGCGCC G[GA]^CG[CT]C HpaI 1127 GTTAAC GTT^AAC HpaII 857 CCGG C^CGG KasI 764 GGCGCC G^GCGCC MamI 1329 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 2639 CAGCTG C[AC]G^C[GT]G MspI 857 CCGG C^CGG NarI 764 GGCGCC GG^CGCC PaeR7I 829 CTCGAG C^TCGAG PflFI 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 856 ACCGGT A^CCGGT PpuMI 1839 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 2639 CAGCTG CAG^CTG SalI 184 GTCGAC G^TCGAC SfoI 764 GGCGCC GGC^GCC SgrAI 855 CACCGGTG C[GA]^CCGG[CT]G Tth111I 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2688 CCAGTGTACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 829 CTCGAG C^TCGAG XmnI 397 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 448 CGCG CG^CG 1977 CGCG AflIII 742 ACATGT A^C[GA][CT]GT 1785 ACATGT AlwNI 5 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 951 CAGAACCTG BciVI 868 GGATAC GTATCC(6/5) 1334 GTATCC BclI 947 TGATCA T^GATCA 2595 TGATCA BseRI 15 CTCCTC GAGGAG(10/8) 129 GAGGAG BsmI 1552 GCATTC GAATGC(1/-1) 1772 GCATTC Bsp1407I 2244 TGTACA T^GTACA 2692 TGTACA BspHI 1006 TCATGA T^CATGA 2598 TCATGA BspLU11I 742 ACATGT A^CATGT 1785 ACATGT BsrGI 2244 TGTACA T^GTACA 2692 TGTACA Bst1107I 367 GTATAC GTA^TAC 746 GTATAC BstUI 448 CGCG CG^CG 1977 CGCG BstYI 163 AGATCT [GA]^GATC[CT] 2481 AGATCC BstZ17I 367 GTATAC GTA^TAC 746 GTATAC CfoI 765 GCGC GCG^C 938 GCGC DraII 1839 GGGTCCT [GA]G^G[ACGT]CC[CT] 2202 AGGCCCC EarI 1822 CTCTTC CTCTTC(1/4) 572 GAAGAG EcoO109I 1839 GGGTCCT [GA]G^G[ACGT]CC[CT] 2202 AGGCCCC EcoT14I 100 CCTTGG C^C[AT][AT]GG 2193 CCTTGG FbaI 947 TGATCA T^GATCA 2595 TGATCA HaeII 764 GGCGCC [GA]GCGC^[CT] 937 GGCGCT HhaI 765 GCGC GCG^C 938 GCGC HinP1I 765 GCGC G^CGC 938 GCGC Ksp632I 1822 CTCTTC CTCTTC(1/4) 572 GAAGAG MaeII 279 ACGT A^CGT 312 ACGT MflI 163 AGATCT [GA]^GATC[CT] 2481 AGATCC MvnI 448 CGCG CG^CG 1977 CGCG PleI 13 GACTC GAGTC(4/5) 2562 GAGTC RcaI 1006 TCATGA T^CATGA 2598 TCATGA SspBI 2244 TGTACA T^GTACA 2692 TGTACA SspI 2335 AATATT AAT^ATT 2757 AATATT StyI 100 CCTTGG C^C[AT][AT]GG 2193 CCTTGG TfiI 79 GATTC G^A[AT]TC 1705 GAATC XhoII 163 AGATCT [GA]^GATC[CT] 2481 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccI AciI AcsI AfaI AluI ApoI AseI AsnI AvaII BanI BbsI BbvI BfaI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmBI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EcoRII EcoT22I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I