##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AviII AvrII BalI BamHI BbrPI
BcgI BcnI BfrI BglI BlnI
BlpI Bpu1102I BseAI BsiEI BsiHKAI
BsiWI BspDI BspEI BsrBI BsrDI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstXI Bsu36I CelII ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FauI
FseI FspI HindIII KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Van91I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 764 GGCGCC G[GA]^CG[CT]C
AgeI 856 ACCGGT A^CCGGT
AlwI 2482 GATCC GGATC(4/5)
Asp700I 397 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 829 CTCGAG C^[CT]CG[GA]G
BanII 1067 GGGCTC G[GA]GC[CT]^C
BbeI 764 GGCGCC GGCGC^C
BbvCI 586 GCTGAGG CCTCAGC(-5/-2)
BglII 163 AGATCT A^GATCT
BmyI 1067 GGGCTC G[AGT]GC[ACT]^C
BsaAI 278 TACGTG [CT]AC^GT[GA]
BsaBI 1329 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 764 GGCGCC G[GA]^CG[CT]C
BsaI 996 GAGACC GGTCTC(1/5)
BsaWI 856 ACCGGT [AT]^CCGG[AT]
BsgI 2270 GTGCAG GTGCAG(16/14)
BsmFI 1400 GGGAC GGGAC(10/14)
BsoBI 829 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1067 GGGCTC G[AGT]GC[ACT]^C
BspMI 955 ACCTGC ACCTGC(4/8)
BsrFI 856 ACCGGT [GA]^CCGG[CT]
Cfr10I 856 ACCGGT [GA]^CCGG[CT]
EcoRV 2602 GATATC GAT^ATC
HapII 857 CCGG C^CGG
HgaI 446 GACGC GACGC(5/10)
Hin1I 764 GGCGCC G[GA]^CG[CT]C
HpaI 1127 GTTAAC GTT^AAC
HpaII 857 CCGG C^CGG
KasI 764 GGCGCC G^GCGCC
MamI 1329 GATGGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 2639 CAGCTG C[AC]G^C[GT]G
MspI 857 CCGG C^CGG
NarI 764 GGCGCC GG^CGCC
PaeR7I 829 CTCGAG C^TCGAG
PflFI 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 856 ACCGGT A^CCGGT
PpuMI 1839 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 2639 CAGCTG CAG^CTG
SalI 184 GTCGAC G^TCGAC
SfoI 764 GGCGCC GGC^GCC
SgrAI 855 CACCGGTG C[GA]^CCGG[CT]G
Tth111I 1997 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2688 CCAGTGTACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 829 CTCGAG C^TCGAG
XmnI 397 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 448 CGCG CG^CG
1977 CGCG
AflIII 742 ACATGT A^C[GA][CT]GT
1785 ACATGT
AlwNI 5 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
951 CAGAACCTG
BciVI 868 GGATAC GTATCC(6/5)
1334 GTATCC
BclI 947 TGATCA T^GATCA
2595 TGATCA
BseRI 15 CTCCTC GAGGAG(10/8)
129 GAGGAG
BsmI 1552 GCATTC GAATGC(1/-1)
1772 GCATTC
Bsp1407I 2244 TGTACA T^GTACA
2692 TGTACA
BspHI 1006 TCATGA T^CATGA
2598 TCATGA
BspLU11I 742 ACATGT A^CATGT
1785 ACATGT
BsrGI 2244 TGTACA T^GTACA
2692 TGTACA
Bst1107I 367 GTATAC GTA^TAC
746 GTATAC
BstUI 448 CGCG CG^CG
1977 CGCG
BstYI 163 AGATCT [GA]^GATC[CT]
2481 AGATCC
BstZ17I 367 GTATAC GTA^TAC
746 GTATAC
CfoI 765 GCGC GCG^C
938 GCGC
DraII 1839 GGGTCCT [GA]G^G[ACGT]CC[CT]
2202 AGGCCCC
EarI 1822 CTCTTC CTCTTC(1/4)
572 GAAGAG
EcoO109I 1839 GGGTCCT [GA]G^G[ACGT]CC[CT]
2202 AGGCCCC
EcoT14I 100 CCTTGG C^C[AT][AT]GG
2193 CCTTGG
FbaI 947 TGATCA T^GATCA
2595 TGATCA
HaeII 764 GGCGCC [GA]GCGC^[CT]
937 GGCGCT
HhaI 765 GCGC GCG^C
938 GCGC
HinP1I 765 GCGC G^CGC
938 GCGC
Ksp632I 1822 CTCTTC CTCTTC(1/4)
572 GAAGAG
MaeII 279 ACGT A^CGT
312 ACGT
MflI 163 AGATCT [GA]^GATC[CT]
2481 AGATCC
MvnI 448 CGCG CG^CG
1977 CGCG
PleI 13 GACTC GAGTC(4/5)
2562 GAGTC
RcaI 1006 TCATGA T^CATGA
2598 TCATGA
SspBI 2244 TGTACA T^GTACA
2692 TGTACA
SspI 2335 AATATT AAT^ATT
2757 AATATT
StyI 100 CCTTGG C^C[AT][AT]GG
2193 CCTTGG
TfiI 79 GATTC G^A[AT]TC
1705 GAATC
XhoII 163 AGATCT [GA]^GATC[CT]
2481 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI AseI AsnI AvaII BanI
BbsI BbvI BfaI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmBI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI EcoRII
EcoT22I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I