################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AccI AccIII AcyI AflII AhdI Alw44I AlwNI ApaI ApaLI AscI AspEI AspI AviII BalI BbeI BbvCI BfrI BglI BlpI Bpu1102I BsaHI BseAI BsiWI BspDI BspEI BspLU11I BsrDI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DrdI Eam1105I EcoO65I EcoRV EcoT22I FseI FspI Hin1I HpaI KasI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PmeI PshAI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XcmI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 2624 GGTACC G^GTACC AclI 712 AACGTT AA^CGTT AgeI 1639 ACCGGT A^CCGGT Aor51HI 140 AGCGCT AGC^GCT Asp718I 2624 GGTACC G^GTACC AspHI 2032 GAGCTC G[AT]GC[AT]^C AvrII 2870 CCTAGG C^CTAGG BciVI 172 GGATAC GTATCC(6/5) BlnI 2870 CCTAGG C^CTAGG BsaBI 586 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1639 ACCGGT [AT]^CCGG[AT] BseRI 2191 CTCCTC GAGGAG(10/8) BsiEI 1755 CGGCCG CG[GA][CT]^CG BsiHKAI 2032 GAGCTC G[AT]GC[AT]^C BsmBI 2282 CGTCTC CGTCTC(1/5) Bsp1407I 2597 TGTACA T^GTACA BspHI 940 TCATGA T^CATGA BspMI 1686 GCAGGT ACCTGC(4/8) BsrBI 231 CCGCTC CCGCTC(-3/-3) BsrGI 2597 TGTACA T^GTACA DraI 840 TTTAAA TTT^AAA DraIII 1662 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1755 CGGCCG C^GGCCG EclXI 1755 CGGCCG C^GGCCG Eco47III 140 AGCGCT AGC^GCT Eco52I 1755 CGGCCG C^GGCCG EcoNI 2603 CCTACCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 2870 CCTAGG C^C[AT][AT]GG HincII 2357 GTCAAC GT[CT]^[GA]AC HindII 2357 GTCAAC GT[CT]^[GA]AC HindIII 573 AAGCTT A^AGCTT KpnI 2624 GGTACC GGTAC^C MamI 586 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 2751 CATATG CA^TATG NspI 1657 GCATGC [GA]CATG^[CT] PaeR7I 896 CTCGAG C^TCGAG PinAI 1639 ACCGGT A^CCGGT Psp1406I 712 AACGTT AA^CGTT PstI 136 CTGCAG CTGCA^G RcaI 940 TCATGA T^CATGA SacI 2032 GAGCTC GAGCT^C SapI 215 GCTCTTC GCTCTTC(1/4) SphI 1657 GCATGC GCATG^C SspBI 2597 TGTACA T^GTACA SspI 1182 AATATT AAT^ATT StyI 2870 CCTAGG C^C[AT][AT]GG XhoI 896 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AocI 434 CCTAAGG CC^T[ACGT]AGG 2607 CCTAAGG AseI 1096 ATTAAT AT^TAAT 2644 ATTAAT AsnI 1096 ATTAAT AT^TAAT 2644 ATTAAT BanI 126 GGTGCC G^G[CT][GA]CC 2624 GGTACC BanII 565 GAGCCC G[GA]GC[CT]^C 2032 GAGCTC BclI 591 TGATCA T^GATCA 968 TGATCA BpmI 562 CTGGAG CTGGAG(16/14) 783 CTGGAG Bsu36I 434 CCTAAGG CC^T[ACGT]AGG 2607 CCTAAGG EaeI 477 TGGCCG [CT]^GGCC[GA] 1755 CGGCCG Eco81I 434 CCTAAGG CC^T[ACGT]AGG 2607 CCTAAGG FbaI 591 TGATCA T^GATCA 968 TGATCA HaeII 140 AGCGCT [GA]GCGC^[CT] 685 GGCGCT KspI 12 CCGCGG CCGC^GG 1938 CCGCGG PshBI 1096 ATTAAT AT^TAAT 2644 ATTAAT SacII 12 CCGCGG CCGC^GG 1938 CCGCGG SfcI 136 CTGCAG C^T[GA][CT]AG 518 CTGTAG SmaI 168 CCCGGG CCC^GGG 450 CCCGGG XbaI 2468 TCTAGA T^CTAGA 3025 TCTAGA XmaCI 168 CCCGGG C^CCGGG 450 CCCGGG XmaI 168 CCCGGG C^CCGGG 450 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AvaI AvaII BamHI BbrPI BbsI BbvI BcgI BcnI BfaI BglII BmyI BpuAI BsaAI BsaI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PmaCI PmlI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SgrAI SmlI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI