##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccI AccIII AcyI AflII
AhdI Alw44I AlwNI ApaI ApaLI
AscI AspEI AspI AviII BalI
BbeI BbvCI BfrI BglI BlpI
Bpu1102I BsaHI BseAI BsiWI BspDI
BspEI BspLU11I BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DrdI Eam1105I
EcoO65I EcoRV EcoT22I FseI FspI
Hin1I HpaI KasI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PmeI PshAI PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I XcmI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2624 GGTACC G^GTACC
AclI 712 AACGTT AA^CGTT
AgeI 1639 ACCGGT A^CCGGT
Aor51HI 140 AGCGCT AGC^GCT
Asp718I 2624 GGTACC G^GTACC
AspHI 2032 GAGCTC G[AT]GC[AT]^C
AvrII 2870 CCTAGG C^CTAGG
BciVI 172 GGATAC GTATCC(6/5)
BlnI 2870 CCTAGG C^CTAGG
BsaBI 586 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1639 ACCGGT [AT]^CCGG[AT]
BseRI 2191 CTCCTC GAGGAG(10/8)
BsiEI 1755 CGGCCG CG[GA][CT]^CG
BsiHKAI 2032 GAGCTC G[AT]GC[AT]^C
BsmBI 2282 CGTCTC CGTCTC(1/5)
Bsp1407I 2597 TGTACA T^GTACA
BspHI 940 TCATGA T^CATGA
BspMI 1686 GCAGGT ACCTGC(4/8)
BsrBI 231 CCGCTC CCGCTC(-3/-3)
BsrGI 2597 TGTACA T^GTACA
DraI 840 TTTAAA TTT^AAA
DraIII 1662 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1755 CGGCCG C^GGCCG
EclXI 1755 CGGCCG C^GGCCG
Eco47III 140 AGCGCT AGC^GCT
Eco52I 1755 CGGCCG C^GGCCG
EcoNI 2603 CCTACCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 2870 CCTAGG C^C[AT][AT]GG
HincII 2357 GTCAAC GT[CT]^[GA]AC
HindII 2357 GTCAAC GT[CT]^[GA]AC
HindIII 573 AAGCTT A^AGCTT
KpnI 2624 GGTACC GGTAC^C
MamI 586 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2751 CATATG CA^TATG
NspI 1657 GCATGC [GA]CATG^[CT]
PaeR7I 896 CTCGAG C^TCGAG
PinAI 1639 ACCGGT A^CCGGT
Psp1406I 712 AACGTT AA^CGTT
PstI 136 CTGCAG CTGCA^G
RcaI 940 TCATGA T^CATGA
SacI 2032 GAGCTC GAGCT^C
SapI 215 GCTCTTC GCTCTTC(1/4)
SphI 1657 GCATGC GCATG^C
SspBI 2597 TGTACA T^GTACA
SspI 1182 AATATT AAT^ATT
StyI 2870 CCTAGG C^C[AT][AT]GG
XhoI 896 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 434 CCTAAGG CC^T[ACGT]AGG
2607 CCTAAGG
AseI 1096 ATTAAT AT^TAAT
2644 ATTAAT
AsnI 1096 ATTAAT AT^TAAT
2644 ATTAAT
BanI 126 GGTGCC G^G[CT][GA]CC
2624 GGTACC
BanII 565 GAGCCC G[GA]GC[CT]^C
2032 GAGCTC
BclI 591 TGATCA T^GATCA
968 TGATCA
BpmI 562 CTGGAG CTGGAG(16/14)
783 CTGGAG
Bsu36I 434 CCTAAGG CC^T[ACGT]AGG
2607 CCTAAGG
EaeI 477 TGGCCG [CT]^GGCC[GA]
1755 CGGCCG
Eco81I 434 CCTAAGG CC^T[ACGT]AGG
2607 CCTAAGG
FbaI 591 TGATCA T^GATCA
968 TGATCA
HaeII 140 AGCGCT [GA]GCGC^[CT]
685 GGCGCT
KspI 12 CCGCGG CCGC^GG
1938 CCGCGG
PshBI 1096 ATTAAT AT^TAAT
2644 ATTAAT
SacII 12 CCGCGG CCGC^GG
1938 CCGCGG
SfcI 136 CTGCAG C^T[GA][CT]AG
518 CTGTAG
SmaI 168 CCCGGG CCC^GGG
450 CCCGGG
XbaI 2468 TCTAGA T^CTAGA
3025 TCTAGA
XmaCI 168 CCCGGG C^CCGGG
450 CCCGGG
XmaI 168 CCCGGG C^CCGGG
450 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AvaI
AvaII BamHI BbrPI BbsI BbvI
BcgI BcnI BfaI BglII BmyI
BpuAI BsaAI BsaI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PmaCI PmlI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SgrAI
SmlI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI