##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp700I
AspEI AviII AvrII BbeI BbrPI
BclI BglI BlnI BsaAI BsiWI
Bsp1407I BspDI BspHI BsrDI BsrGI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI KasI MfeI
MluI MunI NarI NdeI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 1521 GTCTAC GT^[AC][GT]AC
AccIII 1200 TCCGGA T^CCGGA
AcsI 2522 AAATTT [GA]^AATT[CT]
AflII 2397 CTTAAG C^TTAAG
ApoI 2522 AAATTT [GA]^AATT[CT]
AseI 2496 ATTAAT AT^TAAT
AsnI 2496 ATTAAT AT^TAAT
AspI 1515 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 893 GGATCC G^GATCC
BbvCI 1835 GCTGAGG CCTCAGC(-5/-2)
BcgI 1090 GCAGAAATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2481 GTATCC GTATCC(6/5)
BfrI 2397 CTTAAG C^TTAAG
BglII 1056 AGATCT A^GATCT
BsaBI 1051 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1200 TCCGGA T^CCGGA
BseRI 604 CTCCTC GAGGAG(10/8)
BsiEI 65 CGGCCG CG[GA][CT]^CG
BsmI 930 GCATTC GAATGC(1/-1)
BspEI 1200 TCCGGA T^CCGGA
BspMI 408 ACCTGC ACCTGC(4/8)
BsrBI 62 GAGCGG CCGCTC(-3/-3)
BssHII 111 GCGCGC G^CGCGC
BssSI 385 CACGAG CACGAG(-5/-1)
BstEII 182 GGTCACC G^GT[ACGT]ACC
BstPI 182 GGTCACC G^GT[ACGT]ACC
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
EcoO65I 182 GGTCACC G^GT[ACGT]ACC
HaeII 37 AGCGCC [GA]GCGC^[CT]
HincII 2201 GTCAAC GT[CT]^[GA]AC
HindII 2201 GTCAAC GT[CT]^[GA]AC
HindIII 1928 AAGCTT A^AGCTT
KspI 360 CCGCGG CCGC^GG
MamI 1051 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1200 TCCGGA T^CCGGA
NcoI 2694 CCATGG C^CATGG
NheI 1934 GCTAGC G^CTAGC
NotI 64 GCGGCCGC GC^GGCCGC
PflFI 1515 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 418 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 405 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 2496 ATTAAT AT^TAAT
SacII 360 CCGCGG CCGC^GG
SspI 2493 AATATT AAT^ATT
StuI 1899 AGGCCT AGG^CCT
Tth111I 1515 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 418 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 337 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 22 GGTACC G^GTACC
2375 GGTACC
AcyI 741 GGCGTC G[GA]^CG[CT]C
1166 GACGCC
AlwNI 419 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1764 CAGCAGCTG
Asp718I 22 GGTACC G^GTACC
2375 GGTACC
AspHI 724 GAGCTC G[AT]GC[AT]^C
2324 GAGCTC
AvaI 93 CCCGGG C^[CT]CG[GA]G
147 CCCGGG
BalI 1245 TGGCCA TGG^CCA
1789 TGGCCA
BsaHI 741 GGCGTC G[GA]^CG[CT]C
1166 GACGCC
BsaWI 877 ACCGGA [AT]^CCGG[AT]
1200 TCCGGA
BsiHKAI 724 GAGCTC G[AT]GC[AT]^C
2324 GAGCTC
BsmBI 743 CGTCTC CGTCTC(1/5)
842 GAGACG
BsoBI 93 CCCGGG C^[CT]CG[GA]G
147 CCCGGG
BspLU11I 2538 ACATGT A^CATGT
2636 ACATGT
BsrFI 738 GCCGGC [GA]^CCGG[CT]
2303 GCCGGC
Cfr10I 738 GCCGGC [GA]^CCGG[CT]
2303 GCCGGC
DraI 2519 TTTAAA TTT^AAA
2752 TTTAAA
DraII 405 GGGACCT [GA]G^G[ACGT]CC[CT]
1855 AGGCCCC
EcoO109I 405 GGGACCT [GA]G^G[ACGT]CC[CT]
1855 AGGCCCC
Hin1I 741 GGCGTC G[GA]^CG[CT]C
1166 GACGCC
KpnI 22 GGTACC GGTAC^C
2375 GGTACC
MluNI 1245 TGGCCA TGG^CCA
1789 TGGCCA
MscI 1245 TGGCCA TGG^CCA
1789 TGGCCA
NaeI 738 GCCGGC GCC^GGC
2303 GCCGGC
NgoMIV 738 GCCGGC G^CCGGC
2303 GCCGGC
PshAI 205 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2120 GACTCTGGTC
SacI 724 GAGCTC GAGCT^C
2324 GAGCTC
SapI 1031 GAAGAGC GCTCTTC(1/4)
1081 GAAGAGC
ScaI 327 AGTACT AGT^ACT
1104 AGTACT
SmaI 93 CCCGGG CCC^GGG
147 CCCGGG
TaqI 1823 TCGA T^CGA
2719 TCGA
TthHB8I 1823 TCGA T^CGA
2719 TCGA
XcmI 2186 CCAGGGCACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2318 CCACCTGAGCTCTGG
XmaCI 93 CCCGGG C^CCGGG
147 CCCGGG
XmaI 93 CCCGGG C^CCGGG
147 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AocI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI Eco81I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII