################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I AspEI AviII AvrII BbeI BbrPI BclI BglI BlnI BsaAI BsiWI Bsp1407I BspDI BspHI BsrDI BsrGI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraIII DrdI Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KasI MfeI MluI MunI NarI NdeI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 1521 GTCTAC GT^[AC][GT]AC AccIII 1200 TCCGGA T^CCGGA AcsI 2522 AAATTT [GA]^AATT[CT] AflII 2397 CTTAAG C^TTAAG ApoI 2522 AAATTT [GA]^AATT[CT] AseI 2496 ATTAAT AT^TAAT AsnI 2496 ATTAAT AT^TAAT AspI 1515 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 893 GGATCC G^GATCC BbvCI 1835 GCTGAGG CCTCAGC(-5/-2) BcgI 1090 GCAGAAATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2481 GTATCC GTATCC(6/5) BfrI 2397 CTTAAG C^TTAAG BglII 1056 AGATCT A^GATCT BsaBI 1051 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1200 TCCGGA T^CCGGA BseRI 604 CTCCTC GAGGAG(10/8) BsiEI 65 CGGCCG CG[GA][CT]^CG BsmI 930 GCATTC GAATGC(1/-1) BspEI 1200 TCCGGA T^CCGGA BspMI 408 ACCTGC ACCTGC(4/8) BsrBI 62 GAGCGG CCGCTC(-3/-3) BssHII 111 GCGCGC G^CGCGC BssSI 385 CACGAG CACGAG(-5/-1) BstEII 182 GGTCACC G^GT[ACGT]ACC BstPI 182 GGTCACC G^GT[ACGT]ACC EagI 65 CGGCCG C^GGCCG EclXI 65 CGGCCG C^GGCCG Eco52I 65 CGGCCG C^GGCCG EcoO65I 182 GGTCACC G^GT[ACGT]ACC HaeII 37 AGCGCC [GA]GCGC^[CT] HincII 2201 GTCAAC GT[CT]^[GA]AC HindII 2201 GTCAAC GT[CT]^[GA]AC HindIII 1928 AAGCTT A^AGCTT KspI 360 CCGCGG CCGC^GG MamI 1051 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1200 TCCGGA T^CCGGA NcoI 2694 CCATGG C^CATGG NheI 1934 GCTAGC G^CTAGC NotI 64 GCGGCCGC GC^GGCCGC PflFI 1515 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 418 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 405 GGGACCT [GA]G^G[AT]CC[CT] PshBI 2496 ATTAAT AT^TAAT SacII 360 CCGCGG CCGC^GG SspI 2493 AATATT AAT^ATT StuI 1899 AGGCCT AGG^CCT Tth111I 1515 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 418 CCAGCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 337 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 22 GGTACC G^GTACC 2375 GGTACC AcyI 741 GGCGTC G[GA]^CG[CT]C 1166 GACGCC AlwNI 419 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1764 CAGCAGCTG Asp718I 22 GGTACC G^GTACC 2375 GGTACC AspHI 724 GAGCTC G[AT]GC[AT]^C 2324 GAGCTC AvaI 93 CCCGGG C^[CT]CG[GA]G 147 CCCGGG BalI 1245 TGGCCA TGG^CCA 1789 TGGCCA BsaHI 741 GGCGTC G[GA]^CG[CT]C 1166 GACGCC BsaWI 877 ACCGGA [AT]^CCGG[AT] 1200 TCCGGA BsiHKAI 724 GAGCTC G[AT]GC[AT]^C 2324 GAGCTC BsmBI 743 CGTCTC CGTCTC(1/5) 842 GAGACG BsoBI 93 CCCGGG C^[CT]CG[GA]G 147 CCCGGG BspLU11I 2538 ACATGT A^CATGT 2636 ACATGT BsrFI 738 GCCGGC [GA]^CCGG[CT] 2303 GCCGGC Cfr10I 738 GCCGGC [GA]^CCGG[CT] 2303 GCCGGC DraI 2519 TTTAAA TTT^AAA 2752 TTTAAA DraII 405 GGGACCT [GA]G^G[ACGT]CC[CT] 1855 AGGCCCC EcoO109I 405 GGGACCT [GA]G^G[ACGT]CC[CT] 1855 AGGCCCC Hin1I 741 GGCGTC G[GA]^CG[CT]C 1166 GACGCC KpnI 22 GGTACC GGTAC^C 2375 GGTACC MluNI 1245 TGGCCA TGG^CCA 1789 TGGCCA MscI 1245 TGGCCA TGG^CCA 1789 TGGCCA NaeI 738 GCCGGC GCC^GGC 2303 GCCGGC NgoMIV 738 GCCGGC G^CCGGC 2303 GCCGGC PshAI 205 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2120 GACTCTGGTC SacI 724 GAGCTC GAGCT^C 2324 GAGCTC SapI 1031 GAAGAGC GCTCTTC(1/4) 1081 GAAGAGC ScaI 327 AGTACT AGT^ACT 1104 AGTACT SmaI 93 CCCGGG CCC^GGG 147 CCCGGG TaqI 1823 TCGA T^CGA 2719 TCGA TthHB8I 1823 TCGA T^CGA 2719 TCGA XcmI 2186 CCAGGGCACATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2318 CCACCTGAGCTCTGG XmaCI 93 CCCGGG C^CCGGG 147 CCCGGG XmaI 93 CCCGGG C^CCGGG 147 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AocI AvaII BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DsaI EaeI EarI Eco81I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII