##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccII AclI AflIII Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII AvrII BanI BbeI
BbrPI BcgI BciVI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI BspDI
BspLU11I BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I CelII ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MfeI MluI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2339 GTCTAC GT^[AC][GT]AC
AflII 1807 CTTAAG C^TTAAG
AgeI 1248 ACCGGT A^CCGGT
ApaI 3417 GGGCCC GGGCC^C
BfrI 1807 CTTAAG C^TTAAG
BsaBI 229 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2601 GTGCAG GTGCAG(16/14)
BsrBI 3272 GAGCGG CCGCTC(-3/-3)
BsrDI 911 GCAATG GCAATG(2/0)
DraI 2 TTTAAA TTT^AAA
DrdI 3472 GACTCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2281 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1743 CCATGG C^C[AT][AT]GG
HincII 3311 GTTGAC GT[CT]^[GA]AC
HindII 3311 GTTGAC GT[CT]^[GA]AC
MamI 229 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1743 CCATGG C^CATGG
PaeR7I 481 CTCGAG C^TCGAG
PinAI 1248 ACCGGT A^CCGGT
PshAI 1604 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1140 ACCAGGT A^CC[AT]GGT
SfiI 2267 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2802 CCCGGG CCC^GGG
SspI 2168 AATATT AAT^ATT
StyI 1743 CCATGG C^C[AT][AT]GG
XhoI 481 CTCGAG C^TCGAG
XmaCI 2802 CCCGGG C^CCGGG
XmaI 2802 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1163 GACGTC GACGT^C
1646 GACGTC
AccIII 2382 TCCGGA T^CCGGA
2757 TCCGGA
AcyI 1163 GACGTC G[GA]^CG[CT]C
1646 GACGTC
AhdI 260 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2210 GACACCTTGTC
AocI 376 CCTGAGG CC^T[ACGT]AGG
3256 CCTCAGG
AspEI 260 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2210 GACACCTTGTC
AspI 1127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3561 GACCATGTC
AvaI 481 CTCGAG C^[CT]CG[GA]G
2802 CCCGGG
BbvCI 605 GCTGAGG CCTCAGC(-5/-2)
3124 GCTGAGG
BfaI 2573 CTAG C^TAG
3345 CTAG
BglI 2096 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2268 GCCTGGTGGGC
BglII 2559 AGATCT A^GATCT
3341 AGATCT
BsaHI 1163 GACGTC G[GA]^CG[CT]C
1646 GACGTC
BsaI 1566 GAGACC GGTCTC(1/5)
2797 GAGACC
BseAI 2382 TCCGGA T^CCGGA
2757 TCCGGA
BsmBI 1161 GAGACG CGTCTC(1/5)
2324 GAGACG
BsoBI 481 CTCGAG C^[CT]CG[GA]G
2802 CCCGGG
BspEI 2382 TCCGGA T^CCGGA
2757 TCCGGA
BstEII 166 GGTCACC G^GT[ACGT]ACC
2728 GGTGACC
BstPI 166 GGTCACC G^GT[ACGT]ACC
2728 GGTGACC
Bsu36I 376 CCTGAGG CC^T[ACGT]AGG
3256 CCTCAGG
Eam1105I 260 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2210 GACACCTTGTC
Eco81I 376 CCTGAGG CC^T[ACGT]AGG
3256 CCTCAGG
EcoO65I 166 GGTCACC G^GT[ACGT]ACC
2728 GGTGACC
HgaI 758 GACGC GACGC(5/10)
2154 GCGTC
Hin1I 1163 GACGTC G[GA]^CG[CT]C
1646 GACGTC
MaeI 2573 CTAG C^TAG
3345 CTAG
MroI 2382 TCCGGA T^CCGGA
2757 TCCGGA
PflFI 1127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3561 GACCATGTC
SacI 2297 GAGCTC GAGCT^C
3287 GAGCTC
SphI 2086 GCATGC GCATG^C
2118 GCATGC
Tth111I 1127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3561 GACCATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BamHI BanII BbsI BbvI
BclI BcnI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BspMI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRI EcoRII FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI