################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccII AclI AflIII Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AviII AvrII BanI BbeI BbrPI BcgI BciVI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BspDI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I CelII ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HaeII HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccI 2339 GTCTAC GT^[AC][GT]AC AflII 1807 CTTAAG C^TTAAG AgeI 1248 ACCGGT A^CCGGT ApaI 3417 GGGCCC GGGCC^C BfrI 1807 CTTAAG C^TTAAG BsaBI 229 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 2601 GTGCAG GTGCAG(16/14) BsrBI 3272 GAGCGG CCGCTC(-3/-3) BsrDI 911 GCAATG GCAATG(2/0) DraI 2 TTTAAA TTT^AAA DrdI 3472 GACTCCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2281 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1743 CCATGG C^C[AT][AT]GG HincII 3311 GTTGAC GT[CT]^[GA]AC HindII 3311 GTTGAC GT[CT]^[GA]AC MamI 229 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1743 CCATGG C^CATGG PaeR7I 481 CTCGAG C^TCGAG PinAI 1248 ACCGGT A^CCGGT PshAI 1604 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SexAI 1140 ACCAGGT A^CC[AT]GGT SfiI 2267 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 2802 CCCGGG CCC^GGG SspI 2168 AATATT AAT^ATT StyI 1743 CCATGG C^C[AT][AT]GG XhoI 481 CTCGAG C^TCGAG XmaCI 2802 CCCGGG C^CCGGG XmaI 2802 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 1163 GACGTC GACGT^C 1646 GACGTC AccIII 2382 TCCGGA T^CCGGA 2757 TCCGGA AcyI 1163 GACGTC G[GA]^CG[CT]C 1646 GACGTC AhdI 260 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2210 GACACCTTGTC AocI 376 CCTGAGG CC^T[ACGT]AGG 3256 CCTCAGG AspEI 260 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2210 GACACCTTGTC AspI 1127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3561 GACCATGTC AvaI 481 CTCGAG C^[CT]CG[GA]G 2802 CCCGGG BbvCI 605 GCTGAGG CCTCAGC(-5/-2) 3124 GCTGAGG BfaI 2573 CTAG C^TAG 3345 CTAG BglI 2096 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2268 GCCTGGTGGGC BglII 2559 AGATCT A^GATCT 3341 AGATCT BsaHI 1163 GACGTC G[GA]^CG[CT]C 1646 GACGTC BsaI 1566 GAGACC GGTCTC(1/5) 2797 GAGACC BseAI 2382 TCCGGA T^CCGGA 2757 TCCGGA BsmBI 1161 GAGACG CGTCTC(1/5) 2324 GAGACG BsoBI 481 CTCGAG C^[CT]CG[GA]G 2802 CCCGGG BspEI 2382 TCCGGA T^CCGGA 2757 TCCGGA BstEII 166 GGTCACC G^GT[ACGT]ACC 2728 GGTGACC BstPI 166 GGTCACC G^GT[ACGT]ACC 2728 GGTGACC Bsu36I 376 CCTGAGG CC^T[ACGT]AGG 3256 CCTCAGG Eam1105I 260 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2210 GACACCTTGTC Eco81I 376 CCTGAGG CC^T[ACGT]AGG 3256 CCTCAGG EcoO65I 166 GGTCACC G^GT[ACGT]ACC 2728 GGTGACC HgaI 758 GACGC GACGC(5/10) 2154 GCGTC Hin1I 1163 GACGTC G[GA]^CG[CT]C 1646 GACGTC MaeI 2573 CTAG C^TAG 3345 CTAG MroI 2382 TCCGGA T^CCGGA 2757 TCCGGA PflFI 1127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3561 GACCATGTC SacI 2297 GAGCTC GAGCT^C 3287 GAGCTC SphI 2086 GCATGC GCATG^C 2118 GCATGC Tth111I 1127 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3561 GACCATGTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BamHI BanII BbsI BbvI BclI BcnI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspMI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII FauI FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI