##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI ApaI AscI Asp718I AspEI
AviII BamHI BanI BanII BbeI
BbrPI BcgI BclI BglI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV FbaI FseI
FspI HgaI HpaI KasI KpnI
KspI MamI MfeI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1302 GACGTC GACGT^C
AcyI 1302 GACGTC G[GA]^CG[CT]C
AflII 1024 CTTAAG C^TTAAG
Alw44I 1972 GTGCAC G^TGCAC
AocI 616 CCTTAGG CC^T[ACGT]AGG
Aor51HI 1194 AGCGCT AGC^GCT
ApaLI 1972 GTGCAC G^TGCAC
AvaI 235 CTCGGG C^[CT]CG[GA]G
AvaII 2058 GGTCC G^G[AT]CC
AvrII 434 CCTAGG C^CTAGG
BbvCI 844 GCTGAGG CCTCAGC(-5/-2)
BciVI 88 GGATAC GTATCC(6/5)
BfrI 1024 CTTAAG C^TTAAG
BglII 3170 AGATCT A^GATCT
BlnI 434 CCTAGG C^CTAGG
BsaAI 2779 TACGTA [CT]AC^GT[GA]
BsaHI 1302 GACGTC G[GA]^CG[CT]C
BsmBI 968 CGTCTC CGTCTC(1/5)
BsoBI 235 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2518 TGTACA T^GTACA
BspLU11I 889 ACATGT A^CATGT
BsrFI 2075 GCCGGT [GA]^CCGG[CT]
BsrGI 2518 TGTACA T^GTACA
BssHII 1706 GCGCGC G^CGCGC
Bsu36I 616 CCTTAGG CC^T[ACGT]AGG
Cfr10I 2075 GCCGGT [GA]^CCGG[CT]
DraII 2057 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1816 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2012 GACATCATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 553 CCATGG C^C[GA][CT]GG
Eco47III 1194 AGCGCT AGC^GCT
Eco81I 616 CCTTAGG CC^T[ACGT]AGG
EcoO109I 2057 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1811 GAATTC G^AATTC
Hin1I 1302 GACGTC G[GA]^CG[CT]C
MluI 1326 ACGCGT A^CGCGT
NcoI 553 CCATGG C^CATGG
PpuMI 2057 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1399 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SnaBI 2779 TACGTA TAC^GTA
SspBI 2518 TGTACA T^GTACA
StuI 2107 AGGCCT AGG^CCT
SwaI 3659 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 889 ACATGT A^C[GA][CT]GT
1326 ACGCGT
AlwI 438 GGATC GGATC(4/5)
2686 GGATC
AlwNI 751 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
831 CAGTTACTG
Asp700I 1877 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2338 GAAATTTTTC
AspI 1299 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1656 GACCTTGTC
BalI 490 TGGCCA TGG^CCA
2000 TGGCCA
BcnI 282 CCGGG CC^[GC]GG
1497 CCGGG
BsgI 748 GTGCAG GTGCAG(16/14)
1212 GTGCAG
BsmFI 547 GTCCC GGGAC(10/14)
2561 GTCCC
BsmI 240 GAATGC GAATGC(1/-1)
1563 GAATGC
BspMI 1721 ACCTGC ACCTGC(4/8)
1123 GCAGGT
BsrDI 321 GCAATG GCAATG(2/0)
603 GCAATG
BstAPI 1974 GCACCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1983 GCATGTAGTGC
BstXI 856 CCAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3070 CCAGGCAACTGG
Cfr13I 1489 GGGCC G^G[ACGT]CC
2058 GGTCC
EcoNI 1722 CCTGCGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2475 CCTGAGCCAGG
FauI 787 CCCGC CCCGC(4/6)
1137 CCCGC
HaeII 1194 AGCGCT [GA]GCGC^[CT]
1222 GGCGCT
HincII 1317 GTTGAC GT[CT]^[GA]AC
3219 GTTGAC
HindII 1317 GTTGAC GT[CT]^[GA]AC
3219 GTTGAC
MluNI 490 TGGCCA TGG^CCA
2000 TGGCCA
MscI 490 TGGCCA TGG^CCA
2000 TGGCCA
MspA1I 122 CAGCTG C[AC]G^C[GT]G
2542 CAGCTG
NciI 282 CCGGG CC^[GC]GG
1497 CCGGG
NlaIV 278 GGAACC GG[ACGT]^[ACGT]CC
2057 GGGTCC
PflFI 1299 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1656 GACCTTGTC
PvuII 122 CAGCTG CAG^CTG
2542 CAGCTG
Sau96I 1489 GGGCC G^G[ACGT]CC
2058 GGTCC
TaqI 83 TCGA T^CGA
1112 TCGA
TfiI 1114 GAATC G^A[AT]TC
2154 GAATC
Tth111I 1299 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1656 GACCTTGTC
TthHB8I 83 TCGA T^CGA
1112 TCGA
XbaI 3138 TCTAGA T^CTAGA
3630 TCTAGA
XmnI 1877 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2338 GAAATTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AseI AsnI AspHI BbsI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NdeII NlaIII NsiI NspI
PleI PshBI PstI RsaI Sau3AI
ScrFI SfaNI SfcI SmlI SspI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII