################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## Acc65I AccI AccIII AclI AgeI AhdI ApaI AscI Asp718I AspEI AviII BamHI BanI BanII BbeI BbrPI BcgI BclI BglI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrBI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FbaI FseI FspI HgaI HpaI KasI KpnI KspI MamI MfeI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 1302 GACGTC GACGT^C AcyI 1302 GACGTC G[GA]^CG[CT]C AflII 1024 CTTAAG C^TTAAG Alw44I 1972 GTGCAC G^TGCAC AocI 616 CCTTAGG CC^T[ACGT]AGG Aor51HI 1194 AGCGCT AGC^GCT ApaLI 1972 GTGCAC G^TGCAC AvaI 235 CTCGGG C^[CT]CG[GA]G AvaII 2058 GGTCC G^G[AT]CC AvrII 434 CCTAGG C^CTAGG BbvCI 844 GCTGAGG CCTCAGC(-5/-2) BciVI 88 GGATAC GTATCC(6/5) BfrI 1024 CTTAAG C^TTAAG BglII 3170 AGATCT A^GATCT BlnI 434 CCTAGG C^CTAGG BsaAI 2779 TACGTA [CT]AC^GT[GA] BsaHI 1302 GACGTC G[GA]^CG[CT]C BsmBI 968 CGTCTC CGTCTC(1/5) BsoBI 235 CTCGGG C^[CT]CG[GA]G Bsp1407I 2518 TGTACA T^GTACA BspLU11I 889 ACATGT A^CATGT BsrFI 2075 GCCGGT [GA]^CCGG[CT] BsrGI 2518 TGTACA T^GTACA BssHII 1706 GCGCGC G^CGCGC Bsu36I 616 CCTTAGG CC^T[ACGT]AGG Cfr10I 2075 GCCGGT [GA]^CCGG[CT] DraII 2057 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 1816 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2012 GACATCATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 553 CCATGG C^C[GA][CT]GG Eco47III 1194 AGCGCT AGC^GCT Eco81I 616 CCTTAGG CC^T[ACGT]AGG EcoO109I 2057 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 1811 GAATTC G^AATTC Hin1I 1302 GACGTC G[GA]^CG[CT]C MluI 1326 ACGCGT A^CGCGT NcoI 553 CCATGG C^CATGG PpuMI 2057 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 1399 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SnaBI 2779 TACGTA TAC^GTA SspBI 2518 TGTACA T^GTACA StuI 2107 AGGCCT AGG^CCT SwaI 3659 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AflIII 889 ACATGT A^C[GA][CT]GT 1326 ACGCGT AlwI 438 GGATC GGATC(4/5) 2686 GGATC AlwNI 751 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG 831 CAGTTACTG Asp700I 1877 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2338 GAAATTTTTC AspI 1299 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1656 GACCTTGTC BalI 490 TGGCCA TGG^CCA 2000 TGGCCA BcnI 282 CCGGG CC^[GC]GG 1497 CCGGG BsgI 748 GTGCAG GTGCAG(16/14) 1212 GTGCAG BsmFI 547 GTCCC GGGAC(10/14) 2561 GTCCC BsmI 240 GAATGC GAATGC(1/-1) 1563 GAATGC BspMI 1721 ACCTGC ACCTGC(4/8) 1123 GCAGGT BsrDI 321 GCAATG GCAATG(2/0) 603 GCAATG BstAPI 1974 GCACCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1983 GCATGTAGTGC BstXI 856 CCAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3070 CCAGGCAACTGG Cfr13I 1489 GGGCC G^G[ACGT]CC 2058 GGTCC EcoNI 1722 CCTGCGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2475 CCTGAGCCAGG FauI 787 CCCGC CCCGC(4/6) 1137 CCCGC HaeII 1194 AGCGCT [GA]GCGC^[CT] 1222 GGCGCT HincII 1317 GTTGAC GT[CT]^[GA]AC 3219 GTTGAC HindII 1317 GTTGAC GT[CT]^[GA]AC 3219 GTTGAC MluNI 490 TGGCCA TGG^CCA 2000 TGGCCA MscI 490 TGGCCA TGG^CCA 2000 TGGCCA MspA1I 122 CAGCTG C[AC]G^C[GT]G 2542 CAGCTG NciI 282 CCGGG CC^[GC]GG 1497 CCGGG NlaIV 278 GGAACC GG[ACGT]^[ACGT]CC 2057 GGGTCC PflFI 1299 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1656 GACCTTGTC PvuII 122 CAGCTG CAG^CTG 2542 CAGCTG Sau96I 1489 GGGCC G^G[ACGT]CC 2058 GGTCC TaqI 83 TCGA T^CGA 1112 TCGA TfiI 1114 GAATC G^A[AT]TC 2154 GAATC Tth111I 1299 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC 1656 GACCTTGTC TthHB8I 83 TCGA T^CGA 1112 TCGA XbaI 3138 TCTAGA T^CTAGA 3630 TCTAGA XmnI 1877 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2338 GAAATTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcsI AfaI AluI ApoI AseI AsnI AspHI BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI DdeI DpnII DraI EaeI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NsiI NspI PleI PshBI PstI RsaI Sau3AI ScrFI SfaNI SfcI SmlI SspI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII