##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AflII AflIII AgeI
Alw44I AocI ApaLI AscI AseI
AsnI AvrII BamHI BbrPI BbvCI
BcgI BfrI BlnI BsaAI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DrdI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI FseI HindIII
KspI MfeI MluI MroI MspA1I
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 154 GTCTAC GT^[AC][GT]AC
AccII 1034 CGCG CG^CG
AclI 569 AACGTT AA^CGTT
AhdI 2833 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 145 AGCGCT AGC^GCT
ApaI 2354 GGGCCC GGGCC^C
Asp700I 1114 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2833 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1540 CCCGAG C^[CT]CG[GA]G
AviII 2445 TGCGCA TGC^GCA
BciVI 813 GGATAC GTATCC(6/5)
BclI 1485 TGATCA T^GATCA
BcnI 481 CCCGG CC^[GC]GG
BglI 1412 GCCAACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 518 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 518 GCTGAGC GC^T[ACGT]AGC
BsaI 604 GGTCTC GGTCTC(1/5)
BsaWI 903 ACCGGA [AT]^CCGG[AT]
BsoBI 1540 CCCGAG C^[CT]CG[GA]G
BspHI 2129 TCATGA T^CATGA
BstAPI 2437 GCATCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1034 CGCG CG^CG
CelII 518 GCTGAGC GC^T[ACGT]AGC
DraI 1923 TTTAAA TTT^AAA
DraIII 2295 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2833 GACATTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 145 AGCGCT AGC^GCT
EcoRV 1736 GATATC GAT^ATC
EcoT22I 1854 ATGCAT ATGCA^T
FauI 1166 CCCGC CCCGC(4/6)
FbaI 1485 TGATCA T^GATCA
FspI 2445 TGCGCA TGC^GCA
HgaI 2961 GACGC GACGC(5/10)
HpaI 2717 GTTAAC GTT^AAC
MvnI 1034 CGCG CG^CG
NciI 481 CCCGG CC^[GC]GG
NsiI 1854 ATGCAT ATGCA^T
Psp1406I 569 AACGTT AA^CGTT
RcaI 2129 TCATGA T^CATGA
SacI 2891 GAGCTC GAGCT^C
SapI 587 GAAGAGC GCTCTTC(1/4)
ScaI 2362 AGTACT AGT^ACT
SphI 301 GCATGC GCATG^C
SspI 2222 AATATT AAT^ATT
XmnI 1114 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 320 GGTACC G^GTACC
1609 GGTACC
Asp718I 320 GGTACC G^GTACC
1609 GGTACC
AspI 677 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2287 GACCACGTC
BbeI 413 GGCGCC GGCGC^C
1354 GGCGCC
BglII 337 AGATCT A^GATCT
2276 AGATCT
BsaBI 551 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1868 GATGTGTATC
BsmBI 728 GAGACG CGTCTC(1/5)
2133 GAGACG
BsrDI 2411 CATTGC GCAATG(2/0)
2739 CATTGC
BssSI 2844 CTCGTG CACGAG(-5/-1)
3014 CTCGTG
BstXI 1344 CCACAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2722 CCAGAGATGTGG
KasI 413 GGCGCC G^GCGCC
1354 GGCGCC
KpnI 320 GGTACC GGTAC^C
1609 GGTACC
MamI 551 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1868 GATGTGTATC
NarI 413 GGCGCC GG^CGCC
1354 GGCGCC
NspI 301 GCATGC [GA]CATG^[CT]
3121 GCATGT
PflFI 677 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2287 GACCACGTC
SfoI 413 GGCGCC GGC^GCC
1354 GGCGCC
Tth111I 677 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2287 GACCACGTC
XbaI 2815 TCTAGA T^CTAGA
3283 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII