##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AccIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AspI
AvaI AviII AvrII BamHI BbeI
BcgI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspLU11I BssHII BstEII
BstPI ClaI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV FseI
FspI HpaI KasI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI PvuI SacI SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1388 GACGTC GACGT^C
Acc65I 2382 GGTACC G^GTACC
AclI 2788 AACGTT AA^CGTT
AcyI 1388 GACGTC G[GA]^CG[CT]C
AflIII 1505 ACGTGT A^C[GA][CT]GT
AocI 243 CCTCAGG CC^T[ACGT]AGG
ApaI 84 GGGCCC GGGCC^C
Asp700I 1753 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2382 GGTACC G^GTACC
BalI 1497 TGGCCA TGG^CCA
BanII 84 GGGCCC G[GA]GC[CT]^C
BbrPI 1504 CACGTG CAC^GTG
BbvCI 3348 GCTGAGG CCTCAGC(-5/-2)
BciVI 249 GTATCC GTATCC(6/5)
BsaHI 1388 GACGTC G[GA]^CG[CT]C
BsmBI 3150 CGTCTC CGTCTC(1/5)
BsmI 2330 GAATGC GAATGC(1/-1)
BspHI 856 TCATGA T^CATGA
BsrDI 3035 CATTGC GCAATG(2/0)
BsrFI 3016 ACCGGC [GA]^CCGG[CT]
BssSI 590 CTCGTG CACGAG(-5/-1)
Bst1107I 2478 GTATAC GTA^TAC
BstAPI 2635 GCATTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1354 TTCGAA TT^CGAA
BstXI 1644 CCATTCGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2478 GTATAC GTA^TAC
Bsu36I 243 CCTCAGG CC^T[ACGT]AGG
Cfr10I 3016 ACCGGC [GA]^CCGG[CT]
CpoI 31 CGGACCG CG^G[AT]CCG
DrdI 2225 GACTTCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 243 CCTCAGG CC^T[ACGT]AGG
EcoT14I 945 CCATGG C^C[AT][AT]GG
EcoT22I 2332 ATGCAT ATGCA^T
HgaI 221 GCGTC GACGC(5/10)
Hin1I 1388 GACGTC G[GA]^CG[CT]C
HincII 2162 GTTGAC GT[CT]^[GA]AC
HindII 2162 GTTGAC GT[CT]^[GA]AC
KpnI 2382 GGTACC GGTAC^C
MluNI 1497 TGGCCA TGG^CCA
MscI 1497 TGGCCA TGG^CCA
NcoI 945 CCATGG C^CATGG
NdeI 2550 CATATG CA^TATG
NsiI 2332 ATGCAT ATGCA^T
NspI 3193 GCATGT [GA]CATG^[CT]
NspV 1354 TTCGAA TT^CGAA
PleI 1223 GACTC GAGTC(4/5)
PmaCI 1504 CACGTG CAC^GTG
PmlI 1504 CACGTG CAC^GTG
PpuMI 1107 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 2788 AACGTT AA^CGTT
RcaI 856 TCATGA T^CATGA
RsrII 31 CGGACCG CG^G[AT]CCG
SfuI 1354 TTCGAA TT^CGAA
StyI 945 CCATGG C^C[AT][AT]GG
TfiI 521 GAATC G^A[AT]TC
XcmI 3260 CCAGGCTTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1753 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2478 GTATAC GT^[AC][GT]AC
3248 GTAGAC
AflII 692 CTTAAG C^TTAAG
2198 CTTAAG
Aor51HI 2087 AGCGCT AGC^GCT
2358 AGCGCT
AspHI 388 GTGCTC G[AT]GC[AT]^C
2393 GAGCAC
BbsI 434 GTCTTC GAAGAC(2/6)
2234 GTCTTC
BclI 478 TGATCA T^GATCA
1461 TGATCA
BfrI 692 CTTAAG C^TTAAG
2198 CTTAAG
BglI 833 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2725 GCCATCTGGGC
BlpI 1469 GCTCAGC GC^T[ACGT]AGC
2257 GCTAAGC
Bpu1102I 1469 GCTCAGC GC^T[ACGT]AGC
2257 GCTAAGC
BpuAI 434 GTCTTC GAAGAC(2/6)
2234 GTCTTC
BsgI 439 CTGCAC GTGCAG(16/14)
2027 GTGCAG
BsiHKAI 388 GTGCTC G[AT]GC[AT]^C
2393 GAGCAC
BsrBI 111 CCGCTC CCGCTC(-3/-3)
1445 GAGCGG
CelII 1469 GCTCAGC GC^T[ACGT]AGC
2257 GCTAAGC
Eco47III 2087 AGCGCT AGC^GCT
2358 AGCGCT
EcoNI 188 CCTCACGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1375 CCTTAATGAGG
FbaI 478 TGATCA T^GATCA
1461 TGATCA
HinfI 521 GAATC G^A[ACGT]TC
1223 GACTC
HphI 1892 TCACC GGTGA(8/7)
3306 TCACC
KspI 74 CCGCGG CCGC^GG
88 CCGCGG
SacII 74 CCGCGG CCGC^GG
88 CCGCGG
SspI 2427 AATATT AAT^ATT
2861 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BanI
BbvI BcnI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII