################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BalI BanII BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII Cfr10I ClaI CpoI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI FspI HapII HincII HindII HindIII HpaI HpaII KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NciI NcoI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AspHI 870 GTGCTC G[AT]GC[AT]^C BamHI 785 GGATCC G^GATCC BbsI 1160 GAAGAC GAAGAC(2/6) BbvCI 1137 CCTCAGC CCTCAGC(-5/-2) BciVI 1556 GTATCC GTATCC(6/5) BclI 378 TGATCA T^GATCA BpuAI 1160 GAAGAC GAAGAC(2/6) BsaI 1039 GAGACC GGTCTC(1/5) BsiHKAI 870 GTGCTC G[AT]GC[AT]^C BsrBI 41 GAGCGG CCGCTC(-3/-3) Bst1107I 2434 GTATAC GTA^TAC BstZ17I 2434 GTATAC GTA^TAC DraII 679 GGGTCCT [GA]G^G[ACGT]CC[CT] DraIII 2158 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2746 GACTTGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1052 CCACGG C^C[GA][CT]GG EcoO109I 679 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoT22I 2379 ATGCAT ATGCA^T FauI 49 CCCGC CCCGC(4/6) FbaI 378 TGATCA T^GATCA NsiI 2379 ATGCAT ATGCA^T PacI 2896 TTAATTAA TTAAT^TAA PflMI 578 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 679 GGGTCCT [GA]G^G[AT]CC[CT] PstI 633 CTGCAG CTGCA^G SfcI 633 CTGCAG C^T[GA][CT]AG SpeI 2471 ACTAGT A^CTAGT Van91I 578 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2439 CCAAGTATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 49 enzymes) # ################################### AccI 2434 GTATAC GT^[AC][GT]AC 2467 GTAGAC AlwNI 729 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 801 CAGTTCCTG Asp700I 645 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1711 GAATAATTTC AvaI 21 CCCGAG C^[CT]CG[GA]G 858 CCCGAG BanI 45 GGCGCC G^G[CT][GA]CC 777 GGCGCC BbeI 45 GGCGCC GGCGC^C 777 GGCGCC BbrPI 1593 CACGTG CAC^GTG 3066 CACGTG BmyI 870 GTGCTC G[AGT]GC[ACT]^C 1133 GTGCCC BsaAI 1593 CACGTG [CT]AC^GT[GA] 3066 CACGTG BseRI 684 CTCCTC GAGGAG(10/8) 1271 GAGGAG BsgI 2007 CTGCAC GTGCAG(16/14) 1914 GTGCAG BsmFI 739 GGGAC GGGAC(10/14) 1113 GTCCC BsoBI 21 CCCGAG C^[CT]CG[GA]G 858 CCCGAG Bsp1286I 870 GTGCTC G[AGT]GC[ACT]^C 1133 GTGCCC BspHI 84 TCATGA T^CATGA 348 TCATGA BspLU11I 1657 ACATGT A^CATGT 2567 ACATGT BstF5I 451 GGATG GGATG(2/0) 2174 GGATG BstYI 785 GGATCC [GA]^GATC[CT] 2972 AGATCC CfoI 46 GCGC GCG^C 778 GCGC EarI 1078 GAAGAG CTCTTC(1/4) 1292 GAAGAG EcoNI 686 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 768 CCTTGCCCAGG EcoRI 1881 GAATTC G^AATTC 2025 GAATTC EcoT14I 781 CCTTGG C^C[AT][AT]GG 2199 CCTTGG FokI 451 GGATG GGATG(9/13) 2174 GGATG HaeII 45 GGCGCC [GA]GCGC^[CT] 777 GGCGCC HgaI 29 GACGC GACGC(5/10) 722 GACGC HhaI 46 GCGC GCG^C 778 GCGC HinP1I 46 GCGC G^CGC 778 GCGC KasI 45 GGCGCC G^GCGCC 777 GGCGCC Ksp632I 1078 GAAGAG CTCTTC(1/4) 1292 GAAGAG MaeII 1594 ACGT A^CGT 3067 ACGT MflI 785 GGATCC [GA]^GATC[CT] 2972 AGATCC MslI 1620 CATAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2706 CATTTTAGTG MspA1I 60 CAGCTG C[AC]G^C[GT]G 591 CAGCTG NarI 45 GGCGCC GG^CGCC 777 GGCGCC NdeI 931 CATATG CA^TATG 3060 CATATG PleI 548 GACTC GAGTC(4/5) 1163 GACTC PmaCI 1593 CACGTG CAC^GTG 3066 CACGTG PmlI 1593 CACGTG CAC^GTG 3066 CACGTG PvuII 60 CAGCTG CAG^CTG 591 CAGCTG RcaI 84 TCATGA T^CATGA 348 TCATGA ScaI 1196 AGTACT AGT^ACT 1563 AGTACT SfoI 45 GGCGCC GGC^GCC 777 GGCGCC SspI 1519 AATATT AAT^ATT 2694 AATATT StyI 781 CCTTGG C^C[AT][AT]GG 2199 CCTTGG TaqI 142 TCGA T^CGA 997 TCGA TthHB8I 142 TCGA T^CGA 997 TCGA XhoII 785 GGATCC [GA]^GATC[CT] 2972 AGATCC XmnI 645 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1711 GAATAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AseI AsnI AvaII BbvI BfaI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraI EcoRII Fnu4HI HaeIII Hin1I HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI PshBI RsaI Sau3AI Sau96I ScrFI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI