##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BalI
BanII BcgI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII Cfr10I ClaI CpoI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HapII HincII HindII HindIII
HpaI HpaII KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NciI
NcoI NgoMIV NheI NotI NruI
NspV PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfaNI SfiI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 870 GTGCTC G[AT]GC[AT]^C
BamHI 785 GGATCC G^GATCC
BbsI 1160 GAAGAC GAAGAC(2/6)
BbvCI 1137 CCTCAGC CCTCAGC(-5/-2)
BciVI 1556 GTATCC GTATCC(6/5)
BclI 378 TGATCA T^GATCA
BpuAI 1160 GAAGAC GAAGAC(2/6)
BsaI 1039 GAGACC GGTCTC(1/5)
BsiHKAI 870 GTGCTC G[AT]GC[AT]^C
BsrBI 41 GAGCGG CCGCTC(-3/-3)
Bst1107I 2434 GTATAC GTA^TAC
BstZ17I 2434 GTATAC GTA^TAC
DraII 679 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 2158 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2746 GACTTGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1052 CCACGG C^C[GA][CT]GG
EcoO109I 679 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2379 ATGCAT ATGCA^T
FauI 49 CCCGC CCCGC(4/6)
FbaI 378 TGATCA T^GATCA
NsiI 2379 ATGCAT ATGCA^T
PacI 2896 TTAATTAA TTAAT^TAA
PflMI 578 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 679 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 633 CTGCAG CTGCA^G
SfcI 633 CTGCAG C^T[GA][CT]AG
SpeI 2471 ACTAGT A^CTAGT
Van91I 578 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2439 CCAAGTATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 49 enzymes) #
###################################
AccI 2434 GTATAC GT^[AC][GT]AC
2467 GTAGAC
AlwNI 729 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
801 CAGTTCCTG
Asp700I 645 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1711 GAATAATTTC
AvaI 21 CCCGAG C^[CT]CG[GA]G
858 CCCGAG
BanI 45 GGCGCC G^G[CT][GA]CC
777 GGCGCC
BbeI 45 GGCGCC GGCGC^C
777 GGCGCC
BbrPI 1593 CACGTG CAC^GTG
3066 CACGTG
BmyI 870 GTGCTC G[AGT]GC[ACT]^C
1133 GTGCCC
BsaAI 1593 CACGTG [CT]AC^GT[GA]
3066 CACGTG
BseRI 684 CTCCTC GAGGAG(10/8)
1271 GAGGAG
BsgI 2007 CTGCAC GTGCAG(16/14)
1914 GTGCAG
BsmFI 739 GGGAC GGGAC(10/14)
1113 GTCCC
BsoBI 21 CCCGAG C^[CT]CG[GA]G
858 CCCGAG
Bsp1286I 870 GTGCTC G[AGT]GC[ACT]^C
1133 GTGCCC
BspHI 84 TCATGA T^CATGA
348 TCATGA
BspLU11I 1657 ACATGT A^CATGT
2567 ACATGT
BstF5I 451 GGATG GGATG(2/0)
2174 GGATG
BstYI 785 GGATCC [GA]^GATC[CT]
2972 AGATCC
CfoI 46 GCGC GCG^C
778 GCGC
EarI 1078 GAAGAG CTCTTC(1/4)
1292 GAAGAG
EcoNI 686 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
768 CCTTGCCCAGG
EcoRI 1881 GAATTC G^AATTC
2025 GAATTC
EcoT14I 781 CCTTGG C^C[AT][AT]GG
2199 CCTTGG
FokI 451 GGATG GGATG(9/13)
2174 GGATG
HaeII 45 GGCGCC [GA]GCGC^[CT]
777 GGCGCC
HgaI 29 GACGC GACGC(5/10)
722 GACGC
HhaI 46 GCGC GCG^C
778 GCGC
HinP1I 46 GCGC G^CGC
778 GCGC
KasI 45 GGCGCC G^GCGCC
777 GGCGCC
Ksp632I 1078 GAAGAG CTCTTC(1/4)
1292 GAAGAG
MaeII 1594 ACGT A^CGT
3067 ACGT
MflI 785 GGATCC [GA]^GATC[CT]
2972 AGATCC
MslI 1620 CATAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2706 CATTTTAGTG
MspA1I 60 CAGCTG C[AC]G^C[GT]G
591 CAGCTG
NarI 45 GGCGCC GG^CGCC
777 GGCGCC
NdeI 931 CATATG CA^TATG
3060 CATATG
PleI 548 GACTC GAGTC(4/5)
1163 GACTC
PmaCI 1593 CACGTG CAC^GTG
3066 CACGTG
PmlI 1593 CACGTG CAC^GTG
3066 CACGTG
PvuII 60 CAGCTG CAG^CTG
591 CAGCTG
RcaI 84 TCATGA T^CATGA
348 TCATGA
ScaI 1196 AGTACT AGT^ACT
1563 AGTACT
SfoI 45 GGCGCC GGC^GCC
777 GGCGCC
SspI 1519 AATATT AAT^ATT
2694 AATATT
StyI 781 CCTTGG C^C[AT][AT]GG
2199 CCTTGG
TaqI 142 TCGA T^CGA
997 TCGA
TthHB8I 142 TCGA T^CGA
997 TCGA
XhoII 785 GGATCC [GA]^GATC[CT]
2972 AGATCC
XmnI 645 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1711 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaII BbvI BfaI BpmI BsaHI
BsaJI BsiYI BslI BsmAI BsmI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraI EcoRII Fnu4HI
HaeIII Hin1I HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI PshBI RsaI Sau3AI
Sau96I ScrFI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI