##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI ApaI AscI AseI
AsnI Asp718I BalI BbeI BcgI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI EclXI Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI HgaI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI Sse8387I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 318 GTCTAC GT^[AC][GT]AC
AccII 22 CGCG CG^CG
Alw44I 1290 GTGCAC G^TGCAC
AocI 2152 CCTCAGG CC^T[ACGT]AGG
Aor51HI 27 AGCGCT AGC^GCT
ApaLI 1290 GTGCAC G^TGCAC
AspI 1743 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2702 CCCGGG C^[CT]CG[GA]G
BanI 48 GGCACC G^G[CT][GA]CC
BsaBI 390 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 2702 CCCGGG C^[CT]CG[GA]G
BstAPI 1481 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 22 CGCG CG^CG
Bsu36I 2152 CCTCAGG CC^T[ACGT]AGG
DraI 2501 TTTAAA TTT^AAA
DsaI 655 CCATGG C^C[GA][CT]GG
Eco47III 27 AGCGCT AGC^GCT
Eco81I 2152 CCTCAGG CC^T[ACGT]AGG
EcoNI 2522 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2561 GAATTC G^AATTC
HincII 2769 GTTGAC GT[CT]^[GA]AC
HindII 2769 GTTGAC GT[CT]^[GA]AC
HindIII 411 AAGCTT A^AGCTT
MamI 390 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 22 CGCG CG^CG
NcoI 655 CCATGG C^CATGG
PflFI 1743 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 311 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1 GAGCTC GAGCT^C
SmaI 2702 CCCGGG CCC^GGG
SpeI 1595 ACTAGT A^CTAGT
SphI 232 GCATGC GCATG^C
SspI 2093 AATATT AAT^ATT
StuI 1460 AGGCCT AGG^CCT
SwaI 2500 ATTTAAAT ATTT^AAAT
Tth111I 1743 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2702 CCCGGG C^CCGGG
XmaI 2702 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AhdI 81 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
111 GACATTGAGTC
AspEI 81 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
111 GACATTGAGTC
AviII 646 TGCGCA TGC^GCA
2046 TGCGCA
BbrPI 1285 CACGTG CAC^GTG
1779 CACGTG
BbvCI 1568 GCTGAGG CCTCAGC(-5/-2)
1965 GCTGAGG
BsaAI 1285 CACGTG [CT]AC^GT[GA]
1779 CACGTG
BsmBI 302 CGTCTC CGTCTC(1/5)
69 GAGACG
BspLU11I 337 ACATGT A^CATGT
2727 ACATGT
BspMI 915 GCAGGT ACCTGC(4/8)
2305 GCAGGT
BstXI 628 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2023 CCAACAGAGTGG
Eam1105I 81 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
111 GACATTGAGTC
FspI 646 TGCGCA TGC^GCA
2046 TGCGCA
MspA1I 1485 CAGCTG C[AC]G^C[GT]G
1491 CAGCTG
PmaCI 1285 CACGTG CAC^GTG
1779 CACGTG
PmlI 1285 CACGTG CAC^GTG
1779 CACGTG
PvuII 1485 CAGCTG CAG^CTG
1491 CAGCTG
SapI 1093 GAAGAGC GCTCTTC(1/4)
1335 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII AvrII BamHI BanII BbsI
BbvI BciVI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI