################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI ApaI AscI AseI AsnI Asp718I BalI BbeI BcgI BclI BfrI BglI BglII BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FbaI FseI HgaI Hin1I HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI Sse8387I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 318 GTCTAC GT^[AC][GT]AC AccII 22 CGCG CG^CG Alw44I 1290 GTGCAC G^TGCAC AocI 2152 CCTCAGG CC^T[ACGT]AGG Aor51HI 27 AGCGCT AGC^GCT ApaLI 1290 GTGCAC G^TGCAC AspI 1743 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2702 CCCGGG C^[CT]CG[GA]G BanI 48 GGCACC G^G[CT][GA]CC BsaBI 390 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsoBI 2702 CCCGGG C^[CT]CG[GA]G BstAPI 1481 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 22 CGCG CG^CG Bsu36I 2152 CCTCAGG CC^T[ACGT]AGG DraI 2501 TTTAAA TTT^AAA DsaI 655 CCATGG C^C[GA][CT]GG Eco47III 27 AGCGCT AGC^GCT Eco81I 2152 CCTCAGG CC^T[ACGT]AGG EcoNI 2522 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2561 GAATTC G^AATTC HincII 2769 GTTGAC GT[CT]^[GA]AC HindII 2769 GTTGAC GT[CT]^[GA]AC HindIII 411 AAGCTT A^AGCTT MamI 390 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 22 CGCG CG^CG NcoI 655 CCATGG C^CATGG PflFI 1743 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 311 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1 GAGCTC GAGCT^C SmaI 2702 CCCGGG CCC^GGG SpeI 1595 ACTAGT A^CTAGT SphI 232 GCATGC GCATG^C SspI 2093 AATATT AAT^ATT StuI 1460 AGGCCT AGG^CCT SwaI 2500 ATTTAAAT ATTT^AAAT Tth111I 1743 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2702 CCCGGG C^CCGGG XmaI 2702 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AhdI 81 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 111 GACATTGAGTC AspEI 81 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 111 GACATTGAGTC AviII 646 TGCGCA TGC^GCA 2046 TGCGCA BbrPI 1285 CACGTG CAC^GTG 1779 CACGTG BbvCI 1568 GCTGAGG CCTCAGC(-5/-2) 1965 GCTGAGG BsaAI 1285 CACGTG [CT]AC^GT[GA] 1779 CACGTG BsmBI 302 CGTCTC CGTCTC(1/5) 69 GAGACG BspLU11I 337 ACATGT A^CATGT 2727 ACATGT BspMI 915 GCAGGT ACCTGC(4/8) 2305 GCAGGT BstXI 628 CCACCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2023 CCAACAGAGTGG Eam1105I 81 GACAGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 111 GACATTGAGTC FspI 646 TGCGCA TGC^GCA 2046 TGCGCA MspA1I 1485 CAGCTG C[AC]G^C[GT]G 1491 CAGCTG PmaCI 1285 CACGTG CAC^GTG 1779 CACGTG PmlI 1285 CACGTG CAC^GTG 1779 CACGTG PvuII 1485 CAGCTG CAG^CTG 1491 CAGCTG SapI 1093 GAAGAGC GCTCTTC(1/4) 1335 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII AvrII BamHI BanII BbsI BbvI BciVI BcnI BfaI BlnI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI