################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI ApaI AscI Asp718I AspI AviII BbrPI BciVI BfrI BglI BsaAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI EcoNI EcoO65I FseI FspI KpnI MamI MfeI MluI MroI MunI NheI NotI NruI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I RsrII SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AlwNI 2190 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 698 CCTCAGG CC^T[ACGT]AGG Aor51HI 2053 AGCGCT AGC^GCT BalI 681 TGGCCA TGG^CCA BamHI 1068 GGATCC G^GATCC BbeI 7 GGCGCC GGCGC^C BbvCI 2026 GCTGAGG CCTCAGC(-5/-2) BcgI 2496 CGAGCACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 444 GCTCAGC GC^T[ACGT]AGC Bpu1102I 444 GCTCAGC GC^T[ACGT]AGC BsaWI 539 ACCGGA [AT]^CCGG[AT] BsmI 2075 GAATGC GAATGC(1/-1) BspHI 2257 TCATGA T^CATGA BsrDI 2462 GCAATG GCAATG(2/0) BssHII 105 GCGCGC G^CGCGC BstAPI 1982 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1881 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 698 CCTCAGG CC^T[ACGT]AGG CelII 444 GCTCAGC GC^T[ACGT]AGC DraIII 1420 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 117 CGGCCG C^GGCCG EclXI 117 CGGCCG C^GGCCG Eco47III 2053 AGCGCT AGC^GCT Eco52I 117 CGGCCG C^GGCCG Eco81I 698 CCTCAGG CC^T[ACGT]AGG EcoT22I 2151 ATGCAT ATGCA^T HpaI 259 GTTAAC GTT^AAC KasI 7 GGCGCC G^GCGCC MluNI 681 TGGCCA TGG^CCA MscI 681 TGGCCA TGG^CCA NaeI 418 GCCGGC GCC^GGC NarI 7 GGCGCC GG^CGCC NcoI 1881 CCATGG C^CATGG NdeI 1022 CATATG CA^TATG NgoMIV 418 GCCGGC G^CCGGC NsiI 2151 ATGCAT ATGCA^T PaeR7I 2175 CTCGAG C^TCGAG PvuI 596 CGATCG CGAT^CG RcaI 2257 TCATGA T^CATGA SacI 2692 GAGCTC GAGCT^C SalI 1654 GTCGAC G^TCGAC SapI 2110 GCTCTTC GCTCTTC(1/4) SfoI 7 GGCGCC GGC^GCC StuI 2966 AGGCCT AGG^CCT XcmI 1734 CCAGCAATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2175 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 344 GTCTAC GT^[AC][GT]AC 1654 GTCGAC AhdI 1669 GACTATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2334 GACTGTCAGTC Alw44I 1368 GTGCAC G^TGCAC 2161 GTGCAC ApaLI 1368 GTGCAC G^TGCAC 2161 GTGCAC AseI 2383 ATTAAT AT^TAAT 3717 ATTAAT AsnI 2383 ATTAAT AT^TAAT 3717 ATTAAT Asp700I 1725 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2575 GAAGATCTTC AspEI 1669 GACTATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2334 GACTGTCAGTC AvrII 3582 CCTAGG C^CTAGG 3637 CCTAGG BclI 783 TGATCA T^GATCA 3156 TGATCA BlnI 3582 CCTAGG C^CTAGG 3637 CCTAGG BsaI 307 GAGACC GGTCTC(1/5) 2927 GAGACC BsrFI 255 GCCGGT [GA]^CCGG[CT] 418 GCCGGC Cfr10I 255 GCCGGT [GA]^CCGG[CT] 418 GCCGGC Eam1105I 1669 GACTATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2334 GACTGTCAGTC EcoRV 874 GATATC GAT^ATC 3089 GATATC FbaI 783 TGATCA T^GATCA 3156 TGATCA HindIII 518 AAGCTT A^AGCTT 3424 AAGCTT KspI 36 CCGCGG CCGC^GG 323 CCGCGG PshBI 2383 ATTAAT AT^TAAT 3717 ATTAAT PvuII 384 CAGCTG CAG^CTG 1340 CAGCTG SacII 36 CCGCGG CCGC^GG 323 CCGCGG SmaI 3 CCCGGG CCC^GGG 51 CCCGGG XmaCI 3 CCCGGG C^CCGGG 51 CCCGGG XmaI 3 CCCGGG C^CCGGG 51 CCCGGG XmnI 1725 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2575 GAAGATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AatII AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII