##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI ApaI AscI Asp718I AspI
AviII BbrPI BciVI BfrI BglI
BsaAI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EcoNI
EcoO65I FseI FspI KpnI MamI
MfeI MluI MroI MunI NheI
NotI NruI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AlwNI 2190 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 698 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2053 AGCGCT AGC^GCT
BalI 681 TGGCCA TGG^CCA
BamHI 1068 GGATCC G^GATCC
BbeI 7 GGCGCC GGCGC^C
BbvCI 2026 GCTGAGG CCTCAGC(-5/-2)
BcgI 2496 CGAGCACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 444 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 444 GCTCAGC GC^T[ACGT]AGC
BsaWI 539 ACCGGA [AT]^CCGG[AT]
BsmI 2075 GAATGC GAATGC(1/-1)
BspHI 2257 TCATGA T^CATGA
BsrDI 2462 GCAATG GCAATG(2/0)
BssHII 105 GCGCGC G^CGCGC
BstAPI 1982 GCATCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1881 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 698 CCTCAGG CC^T[ACGT]AGG
CelII 444 GCTCAGC GC^T[ACGT]AGC
DraIII 1420 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 117 CGGCCG C^GGCCG
EclXI 117 CGGCCG C^GGCCG
Eco47III 2053 AGCGCT AGC^GCT
Eco52I 117 CGGCCG C^GGCCG
Eco81I 698 CCTCAGG CC^T[ACGT]AGG
EcoT22I 2151 ATGCAT ATGCA^T
HpaI 259 GTTAAC GTT^AAC
KasI 7 GGCGCC G^GCGCC
MluNI 681 TGGCCA TGG^CCA
MscI 681 TGGCCA TGG^CCA
NaeI 418 GCCGGC GCC^GGC
NarI 7 GGCGCC GG^CGCC
NcoI 1881 CCATGG C^CATGG
NdeI 1022 CATATG CA^TATG
NgoMIV 418 GCCGGC G^CCGGC
NsiI 2151 ATGCAT ATGCA^T
PaeR7I 2175 CTCGAG C^TCGAG
PvuI 596 CGATCG CGAT^CG
RcaI 2257 TCATGA T^CATGA
SacI 2692 GAGCTC GAGCT^C
SalI 1654 GTCGAC G^TCGAC
SapI 2110 GCTCTTC GCTCTTC(1/4)
SfoI 7 GGCGCC GGC^GCC
StuI 2966 AGGCCT AGG^CCT
XcmI 1734 CCAGCAATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2175 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 344 GTCTAC GT^[AC][GT]AC
1654 GTCGAC
AhdI 1669 GACTATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2334 GACTGTCAGTC
Alw44I 1368 GTGCAC G^TGCAC
2161 GTGCAC
ApaLI 1368 GTGCAC G^TGCAC
2161 GTGCAC
AseI 2383 ATTAAT AT^TAAT
3717 ATTAAT
AsnI 2383 ATTAAT AT^TAAT
3717 ATTAAT
Asp700I 1725 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2575 GAAGATCTTC
AspEI 1669 GACTATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2334 GACTGTCAGTC
AvrII 3582 CCTAGG C^CTAGG
3637 CCTAGG
BclI 783 TGATCA T^GATCA
3156 TGATCA
BlnI 3582 CCTAGG C^CTAGG
3637 CCTAGG
BsaI 307 GAGACC GGTCTC(1/5)
2927 GAGACC
BsrFI 255 GCCGGT [GA]^CCGG[CT]
418 GCCGGC
Cfr10I 255 GCCGGT [GA]^CCGG[CT]
418 GCCGGC
Eam1105I 1669 GACTATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2334 GACTGTCAGTC
EcoRV 874 GATATC GAT^ATC
3089 GATATC
FbaI 783 TGATCA T^GATCA
3156 TGATCA
HindIII 518 AAGCTT A^AGCTT
3424 AAGCTT
KspI 36 CCGCGG CCGC^GG
323 CCGCGG
PshBI 2383 ATTAAT AT^TAAT
3717 ATTAAT
PvuII 384 CAGCTG CAG^CTG
1340 CAGCTG
SacII 36 CCGCGG CCGC^GG
323 CCGCGG
SmaI 3 CCCGGG CCC^GGG
51 CCCGGG
XmaCI 3 CCCGGG C^CCGGG
51 CCCGGG
XmaI 3 CCCGGG C^CCGGG
51 CCCGGG
XmnI 1725 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2575 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII