################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI AviII BbrPI BcgI BciVI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BspEI BspHI BspLU11I BssSI BstAPI BstBI CpoI EagI Eam1105I EclXI Eco52I EcoRI EcoT22I FseI FspI HincII HindII HindIII HpaI MamI MluI MroI NaeI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflII 3872 CTTAAG C^TTAAG Aor51HI 2140 AGCGCT AGC^GCT ApaI 3218 GGGCCC GGGCC^C AspI 3118 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 172 CCCGGG C^[CT]CG[GA]G BbvCI 93 CCTCAGC CCTCAGC(-5/-2) BclI 3038 TGATCA T^GATCA BfrI 3872 CTTAAG C^TTAAG BglI 262 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 3408 TACGTG [CT]AC^GT[GA] BsiEI 2262 CGATCG CG[GA][CT]^CG BsoBI 172 CCCGGG C^[CT]CG[GA]G BspDI 2264 ATCGAT AT^CGAT BsrFI 1217 ACCGGC [GA]^CCGG[CT] BssHII 35 GCGCGC G^CGCGC Bst1107I 2367 GTATAC GTA^TAC BstZ17I 2367 GTATAC GTA^TAC Cfr10I 1217 ACCGGC [GA]^CCGG[CT] ClaI 2264 ATCGAT AT^CGAT DraI 1984 TTTAAA TTT^AAA DrdI 2480 GACCACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2140 AGCGCT AGC^GCT EcoRV 928 GATATC GAT^ATC FbaI 3038 TGATCA T^GATCA HgaI 1468 GCGTC GACGC(5/10) MfeI 3320 CAATTG C^AATTG MunI 3320 CAATTG C^AATTG NheI 2137 GCTAGC G^CTAGC PflFI 3118 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuI 2262 CGATCG CGAT^CG SacI 2884 GAGCTC GAGCT^C SapI 232 GCTCTTC GCTCTTC(1/4) SexAI 3119 ACCAGGT A^CC[AT]GGT SmaI 172 CCCGGG CCC^GGG SphI 2423 GCATGC GCATG^C TfiI 5 GAATC G^A[AT]TC Tth111I 3118 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2399 TCTAGA T^CTAGA XmaCI 172 CCCGGG C^CCGGG XmaI 172 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 314 GGTACC G^GTACC 2659 GGTACC Asp718I 314 GGTACC G^GTACC 2659 GGTACC AspHI 2459 GTGCTC G[AT]GC[AT]^C 2884 GAGCTC AvrII 526 CCTAGG C^CTAGG 3694 CCTAGG BalI 356 TGGCCA TGG^CCA 2850 TGGCCA BbeI 1232 GGCGCC GGCGC^C 1727 GGCGCC BglII 1626 AGATCT A^GATCT 3605 AGATCT BlnI 526 CCTAGG C^CTAGG 3694 CCTAGG BlpI 500 GCTCAGC GC^T[ACGT]AGC 1768 GCTCAGC Bpu1102I 500 GCTCAGC GC^T[ACGT]AGC 1768 GCTCAGC BsiHKAI 2459 GTGCTC G[AT]GC[AT]^C 2884 GAGCTC Bsp1407I 1904 TGTACA T^GTACA 3779 TGTACA BsrGI 1904 TGTACA T^GTACA 3779 TGTACA BstEII 467 GGTGACC G^GT[ACGT]ACC 3461 GGTGACC BstPI 467 GGTGACC G^GT[ACGT]ACC 3461 GGTGACC CelII 500 GCTCAGC GC^T[ACGT]AGC 1768 GCTCAGC DraIII 485 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 2586 CACCCCGTG EcoO65I 467 GGTGACC G^GT[ACGT]ACC 3461 GGTGACC KasI 1232 GGCGCC G^GCGCC 1727 GGCGCC KpnI 314 GGTACC GGTAC^C 2659 GGTACC KspI 61 CCGCGG CCGC^GG 152 CCGCGG MluNI 356 TGGCCA TGG^CCA 2850 TGGCCA MscI 356 TGGCCA TGG^CCA 2850 TGGCCA NarI 1232 GGCGCC GG^CGCC 1727 GGCGCC PshAI 1309 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3014 GACTGGTGTC SacII 61 CCGCGG CCGC^GG 152 CCGCGG SfoI 1232 GGCGCC GGC^GCC 1727 GGCGCC SmlI 990 CTTGAG C^T[CT][GA]AG 3872 CTTAAG SspBI 1904 TGTACA T^GTACA 3779 TGTACA XcmI 1275 CCATTTCAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3418 CCAAATGGCTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AvaII BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI