##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AviII BbrPI BcgI BciVI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BspEI BspHI BspLU11I BssSI
BstAPI BstBI CpoI EagI Eam1105I
EclXI Eco52I EcoRI EcoT22I FseI
FspI HincII HindII HindIII HpaI
MamI MluI MroI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AflII 3872 CTTAAG C^TTAAG
Aor51HI 2140 AGCGCT AGC^GCT
ApaI 3218 GGGCCC GGGCC^C
AspI 3118 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 172 CCCGGG C^[CT]CG[GA]G
BbvCI 93 CCTCAGC CCTCAGC(-5/-2)
BclI 3038 TGATCA T^GATCA
BfrI 3872 CTTAAG C^TTAAG
BglI 262 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3408 TACGTG [CT]AC^GT[GA]
BsiEI 2262 CGATCG CG[GA][CT]^CG
BsoBI 172 CCCGGG C^[CT]CG[GA]G
BspDI 2264 ATCGAT AT^CGAT
BsrFI 1217 ACCGGC [GA]^CCGG[CT]
BssHII 35 GCGCGC G^CGCGC
Bst1107I 2367 GTATAC GTA^TAC
BstZ17I 2367 GTATAC GTA^TAC
Cfr10I 1217 ACCGGC [GA]^CCGG[CT]
ClaI 2264 ATCGAT AT^CGAT
DraI 1984 TTTAAA TTT^AAA
DrdI 2480 GACCACTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2140 AGCGCT AGC^GCT
EcoRV 928 GATATC GAT^ATC
FbaI 3038 TGATCA T^GATCA
HgaI 1468 GCGTC GACGC(5/10)
MfeI 3320 CAATTG C^AATTG
MunI 3320 CAATTG C^AATTG
NheI 2137 GCTAGC G^CTAGC
PflFI 3118 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2262 CGATCG CGAT^CG
SacI 2884 GAGCTC GAGCT^C
SapI 232 GCTCTTC GCTCTTC(1/4)
SexAI 3119 ACCAGGT A^CC[AT]GGT
SmaI 172 CCCGGG CCC^GGG
SphI 2423 GCATGC GCATG^C
TfiI 5 GAATC G^A[AT]TC
Tth111I 3118 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2399 TCTAGA T^CTAGA
XmaCI 172 CCCGGG C^CCGGG
XmaI 172 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 314 GGTACC G^GTACC
2659 GGTACC
Asp718I 314 GGTACC G^GTACC
2659 GGTACC
AspHI 2459 GTGCTC G[AT]GC[AT]^C
2884 GAGCTC
AvrII 526 CCTAGG C^CTAGG
3694 CCTAGG
BalI 356 TGGCCA TGG^CCA
2850 TGGCCA
BbeI 1232 GGCGCC GGCGC^C
1727 GGCGCC
BglII 1626 AGATCT A^GATCT
3605 AGATCT
BlnI 526 CCTAGG C^CTAGG
3694 CCTAGG
BlpI 500 GCTCAGC GC^T[ACGT]AGC
1768 GCTCAGC
Bpu1102I 500 GCTCAGC GC^T[ACGT]AGC
1768 GCTCAGC
BsiHKAI 2459 GTGCTC G[AT]GC[AT]^C
2884 GAGCTC
Bsp1407I 1904 TGTACA T^GTACA
3779 TGTACA
BsrGI 1904 TGTACA T^GTACA
3779 TGTACA
BstEII 467 GGTGACC G^GT[ACGT]ACC
3461 GGTGACC
BstPI 467 GGTGACC G^GT[ACGT]ACC
3461 GGTGACC
CelII 500 GCTCAGC GC^T[ACGT]AGC
1768 GCTCAGC
DraIII 485 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
2586 CACCCCGTG
EcoO65I 467 GGTGACC G^GT[ACGT]ACC
3461 GGTGACC
KasI 1232 GGCGCC G^GCGCC
1727 GGCGCC
KpnI 314 GGTACC GGTAC^C
2659 GGTACC
KspI 61 CCGCGG CCGC^GG
152 CCGCGG
MluNI 356 TGGCCA TGG^CCA
2850 TGGCCA
MscI 356 TGGCCA TGG^CCA
2850 TGGCCA
NarI 1232 GGCGCC GG^CGCC
1727 GGCGCC
PshAI 1309 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3014 GACTGGTGTC
SacII 61 CCGCGG CCGC^GG
152 CCGCGG
SfoI 1232 GGCGCC GGC^GCC
1727 GGCGCC
SmlI 990 CTTGAG C^T[CT][GA]AG
3872 CTTAAG
SspBI 1904 TGTACA T^GTACA
3779 TGTACA
XcmI 1275 CCATTTCAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3418 CCAAATGGCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI