##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AspI AvrII BfrI BlnI
BsaBI BsiWI BspDI BspHI BssSI
BstBI ClaI CpoI Eam1105I EcoRV
EcoT22I FseI HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RcaI RsrII SalI ScaI SexAI
SfiI SfuI SnaBI SpeI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcsI 783 GAATTC [GA]^AATT[CT]
ApaI 1708 GGGCCC GGGCC^C
ApoI 783 GAATTC [GA]^AATT[CT]
AseI 1187 ATTAAT AT^TAAT
AsnI 1187 ATTAAT AT^TAAT
Asp700I 181 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 83 TGCGCA TGC^GCA
BalI 2086 TGGCCA TGG^CCA
BbeI 3478 GGCGCC GGCGC^C
BcgI 3368 GCAGCACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3065 GGATAC GTATCC(6/5)
BclI 988 TGATCA T^GATCA
BglI 1656 GCCCCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 882 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 882 GCTCAGC GC^T[ACGT]AGC
BsiEI 1663 CGGCCG CG[GA][CT]^CG
BsmBI 2277 GAGACG CGTCTC(1/5)
BspLU11I 2942 ACATGT A^CATGT
BsrBI 2629 CCGCTC CCGCTC(-3/-3)
Bst1107I 3558 GTATAC GTA^TAC
BstAPI 2769 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2802 GGTTACC G^GT[ACGT]ACC
BstPI 2802 GGTTACC G^GT[ACGT]ACC
BstXI 13 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3558 GTATAC GTA^TAC
CelII 882 GCTCAGC GC^T[ACGT]AGC
DraI 2426 TTTAAA TTT^AAA
DraIII 3301 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2786 GACTGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1663 CGGCCG C^GGCCG
EclXI 1663 CGGCCG C^GGCCG
Eco52I 1663 CGGCCG C^GGCCG
EcoO65I 2802 GGTTACC G^GT[ACGT]ACC
EcoRI 783 GAATTC G^AATTC
FbaI 988 TGATCA T^GATCA
FspI 83 TGCGCA TGC^GCA
KasI 3478 GGCGCC G^GCGCC
KspI 1956 CCGCGG CCGC^GG
MluNI 2086 TGGCCA TGG^CCA
MscI 2086 TGGCCA TGG^CCA
NaeI 2930 GCCGGC GCC^GGC
NarI 3478 GGCGCC GG^CGCC
NgoMIV 2930 GCCGGC G^CCGGC
PaeR7I 129 CTCGAG C^TCGAG
PshBI 1187 ATTAAT AT^TAAT
SacI 1445 GAGCTC GAGCT^C
SacII 1956 CCGCGG CCGC^GG
SbfI 2734 CCTGCAGG CCTGCA^GG
SfoI 3478 GGCGCC GGC^GCC
SgrAI 1464 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 129 CTCGAG C^T[CT][GA]AG
SphI 3168 GCATGC GCATG^C
Sse8387I 2734 CCTGCAGG CCTGCA^GG
XhoI 129 CTCGAG C^TCGAG
XmnI 181 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 3296 GTCTAC GT^[AC][GT]AC
3558 GTATAC
AccIII 772 TCCGGA T^CCGGA
1441 TCCGGA
AocI 2561 CCTCAGG CC^T[ACGT]AGG
2637 CCTCAGG
AspHI 1445 GAGCTC G[AT]GC[AT]^C
2859 GTGCTC
BbrPI 346 CACGTG CAC^GTG
2383 CACGTG
BsaAI 346 CACGTG [CT]AC^GT[GA]
2383 CACGTG
BseAI 772 TCCGGA T^CCGGA
1441 TCCGGA
BsiHKAI 1445 GAGCTC G[AT]GC[AT]^C
2859 GTGCTC
BsmI 701 GAATGC GAATGC(1/-1)
3083 GAATGC
BspEI 772 TCCGGA T^CCGGA
1441 TCCGGA
BsrDI 310 CATTGC GCAATG(2/0)
841 CATTGC
Bsu36I 2561 CCTCAGG CC^T[ACGT]AGG
2637 CCTCAGG
Eco81I 2561 CCTCAGG CC^T[ACGT]AGG
2637 CCTCAGG
EcoNI 81 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1324 CCTGGAACAGG
MroI 772 TCCGGA T^CCGGA
1441 TCCGGA
NcoI 624 CCATGG C^CATGG
1168 CCATGG
NspI 2942 ACATGT [GA]CATG^[CT]
3168 GCATGC
PmaCI 346 CACGTG CAC^GTG
2383 CACGTG
PmlI 346 CACGTG CAC^GTG
2383 CACGTG
PshAI 43 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
332 GACACAGGTC
SmaI 1673 CCCGGG CCC^GGG
2922 CCCGGG
XmaCI 1673 CCCGGG C^CCGGG
2922 CCCGGG
XmaI 1673 CCCGGG C^CCGGG
2922 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI AvaI
AvaII BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII