##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        Asp718I     
AspEI       AspI        AvrII       BfrI        BlnI        
BsaBI       BsiWI       BspDI       BspHI       BssSI       
BstBI       ClaI        CpoI        Eam1105I    EcoRV       
EcoT22I     FseI        HpaI        KpnI        MamI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmeI        Psp1406I    PvuI        
RcaI        RsrII       SalI        ScaI        SexAI       
SfiI        SfuI        SnaBI       SpeI        SspI        
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcsI          783  GAATTC               [GA]^AATT[CT]
ApaI         1708  GGGCCC               GGGCC^C
ApoI          783  GAATTC               [GA]^AATT[CT]
AseI         1187  ATTAAT               AT^TAAT
AsnI         1187  ATTAAT               AT^TAAT
Asp700I       181  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII          83  TGCGCA               TGC^GCA
BalI         2086  TGGCCA               TGG^CCA
BbeI         3478  GGCGCC               GGCGC^C
BcgI         3368  GCAGCACTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3065  GGATAC               GTATCC(6/5)
BclI          988  TGATCA               T^GATCA
BglI         1656  GCCCCTTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          882  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      882  GCTCAGC              GC^T[ACGT]AGC
BsiEI        1663  CGGCCG               CG[GA][CT]^CG
BsmBI        2277  GAGACG               CGTCTC(1/5)
BspLU11I     2942  ACATGT               A^CATGT
BsrBI        2629  CCGCTC               CCGCTC(-3/-3)
Bst1107I     3558  GTATAC               GTA^TAC
BstAPI       2769  GCAAGCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2802  GGTTACC              G^GT[ACGT]ACC
BstPI        2802  GGTTACC              G^GT[ACGT]ACC
BstXI          13  CCAGCTGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      3558  GTATAC               GTA^TAC
CelII         882  GCTCAGC              GC^T[ACGT]AGC
DraI         2426  TTTAAA               TTT^AAA
DraIII       3301  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2786  GACTGCAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1663  CGGCCG               C^GGCCG
EclXI        1663  CGGCCG               C^GGCCG
Eco52I       1663  CGGCCG               C^GGCCG
EcoO65I      2802  GGTTACC              G^GT[ACGT]ACC
EcoRI         783  GAATTC               G^AATTC
FbaI          988  TGATCA               T^GATCA
FspI           83  TGCGCA               TGC^GCA
KasI         3478  GGCGCC               G^GCGCC
KspI         1956  CCGCGG               CCGC^GG
MluNI        2086  TGGCCA               TGG^CCA
MscI         2086  TGGCCA               TGG^CCA
NaeI         2930  GCCGGC               GCC^GGC
NarI         3478  GGCGCC               GG^CGCC
NgoMIV       2930  GCCGGC               G^CCGGC
PaeR7I        129  CTCGAG               C^TCGAG
PshBI        1187  ATTAAT               AT^TAAT
SacI         1445  GAGCTC               GAGCT^C
SacII        1956  CCGCGG               CCGC^GG
SbfI         2734  CCTGCAGG             CCTGCA^GG
SfoI         3478  GGCGCC               GGC^GCC
SgrAI        1464  CGCCGGTG             C[GA]^CCGG[CT]G
SmlI          129  CTCGAG               C^T[CT][GA]AG
SphI         3168  GCATGC               GCATG^C
Sse8387I     2734  CCTGCAGG             CCTGCA^GG
XhoI          129  CTCGAG               C^TCGAG
XmnI          181  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI         3296  GTCTAC               GT^[AC][GT]AC
             3558  GTATAC              
AccIII        772  TCCGGA               T^CCGGA
             1441  TCCGGA              
AocI         2561  CCTCAGG              CC^T[ACGT]AGG
             2637  CCTCAGG             
AspHI        1445  GAGCTC               G[AT]GC[AT]^C
             2859  GTGCTC              
BbrPI         346  CACGTG               CAC^GTG
             2383  CACGTG              
BsaAI         346  CACGTG               [CT]AC^GT[GA]
             2383  CACGTG              
BseAI         772  TCCGGA               T^CCGGA
             1441  TCCGGA              
BsiHKAI      1445  GAGCTC               G[AT]GC[AT]^C
             2859  GTGCTC              
BsmI          701  GAATGC               GAATGC(1/-1)
             3083  GAATGC              
BspEI         772  TCCGGA               T^CCGGA
             1441  TCCGGA              
BsrDI         310  CATTGC               GCAATG(2/0)
              841  CATTGC              
Bsu36I       2561  CCTCAGG              CC^T[ACGT]AGG
             2637  CCTCAGG             
Eco81I       2561  CCTCAGG              CC^T[ACGT]AGG
             2637  CCTCAGG             
EcoNI          81  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1324  CCTGGAACAGG         
MroI          772  TCCGGA               T^CCGGA
             1441  TCCGGA              
NcoI          624  CCATGG               C^CATGG
             1168  CCATGG              
NspI         2942  ACATGT               [GA]CATG^[CT]
             3168  GCATGC              
PmaCI         346  CACGTG               CAC^GTG
             2383  CACGTG              
PmlI          346  CACGTG               CAC^GTG
             2383  CACGTG              
PshAI          43  GACCCACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              332  GACACAGGTC          
SmaI         1673  CCCGGG               CCC^GGG
             2922  CCCGGG              
XmaCI        1673  CCCGGG               C^CCGGG
             2922  CCCGGG              
XmaI         1673  CCCGGG               C^CCGGG
             2922  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       Aor51HI     AvaI        
AvaII       BamHI       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BsaWI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspMI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        Eco47III    EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SspBI       StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoII