################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AspI AvrII BfrI BlnI BsaBI BsiWI BspDI BspHI BssSI BstBI ClaI CpoI Eam1105I EcoRV EcoT22I FseI HpaI KpnI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SfuI SnaBI SpeI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcsI 783 GAATTC [GA]^AATT[CT] ApaI 1708 GGGCCC GGGCC^C ApoI 783 GAATTC [GA]^AATT[CT] AseI 1187 ATTAAT AT^TAAT AsnI 1187 ATTAAT AT^TAAT Asp700I 181 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 83 TGCGCA TGC^GCA BalI 2086 TGGCCA TGG^CCA BbeI 3478 GGCGCC GGCGC^C BcgI 3368 GCAGCACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3065 GGATAC GTATCC(6/5) BclI 988 TGATCA T^GATCA BglI 1656 GCCCCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 882 GCTCAGC GC^T[ACGT]AGC Bpu1102I 882 GCTCAGC GC^T[ACGT]AGC BsiEI 1663 CGGCCG CG[GA][CT]^CG BsmBI 2277 GAGACG CGTCTC(1/5) BspLU11I 2942 ACATGT A^CATGT BsrBI 2629 CCGCTC CCGCTC(-3/-3) Bst1107I 3558 GTATAC GTA^TAC BstAPI 2769 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2802 GGTTACC G^GT[ACGT]ACC BstPI 2802 GGTTACC G^GT[ACGT]ACC BstXI 13 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3558 GTATAC GTA^TAC CelII 882 GCTCAGC GC^T[ACGT]AGC DraI 2426 TTTAAA TTT^AAA DraIII 3301 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2786 GACTGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1663 CGGCCG C^GGCCG EclXI 1663 CGGCCG C^GGCCG Eco52I 1663 CGGCCG C^GGCCG EcoO65I 2802 GGTTACC G^GT[ACGT]ACC EcoRI 783 GAATTC G^AATTC FbaI 988 TGATCA T^GATCA FspI 83 TGCGCA TGC^GCA KasI 3478 GGCGCC G^GCGCC KspI 1956 CCGCGG CCGC^GG MluNI 2086 TGGCCA TGG^CCA MscI 2086 TGGCCA TGG^CCA NaeI 2930 GCCGGC GCC^GGC NarI 3478 GGCGCC GG^CGCC NgoMIV 2930 GCCGGC G^CCGGC PaeR7I 129 CTCGAG C^TCGAG PshBI 1187 ATTAAT AT^TAAT SacI 1445 GAGCTC GAGCT^C SacII 1956 CCGCGG CCGC^GG SbfI 2734 CCTGCAGG CCTGCA^GG SfoI 3478 GGCGCC GGC^GCC SgrAI 1464 CGCCGGTG C[GA]^CCGG[CT]G SmlI 129 CTCGAG C^T[CT][GA]AG SphI 3168 GCATGC GCATG^C Sse8387I 2734 CCTGCAGG CCTGCA^GG XhoI 129 CTCGAG C^TCGAG XmnI 181 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 3296 GTCTAC GT^[AC][GT]AC 3558 GTATAC AccIII 772 TCCGGA T^CCGGA 1441 TCCGGA AocI 2561 CCTCAGG CC^T[ACGT]AGG 2637 CCTCAGG AspHI 1445 GAGCTC G[AT]GC[AT]^C 2859 GTGCTC BbrPI 346 CACGTG CAC^GTG 2383 CACGTG BsaAI 346 CACGTG [CT]AC^GT[GA] 2383 CACGTG BseAI 772 TCCGGA T^CCGGA 1441 TCCGGA BsiHKAI 1445 GAGCTC G[AT]GC[AT]^C 2859 GTGCTC BsmI 701 GAATGC GAATGC(1/-1) 3083 GAATGC BspEI 772 TCCGGA T^CCGGA 1441 TCCGGA BsrDI 310 CATTGC GCAATG(2/0) 841 CATTGC Bsu36I 2561 CCTCAGG CC^T[ACGT]AGG 2637 CCTCAGG Eco81I 2561 CCTCAGG CC^T[ACGT]AGG 2637 CCTCAGG EcoNI 81 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1324 CCTGGAACAGG MroI 772 TCCGGA T^CCGGA 1441 TCCGGA NcoI 624 CCATGG C^CATGG 1168 CCATGG NspI 2942 ACATGT [GA]CATG^[CT] 3168 GCATGC PmaCI 346 CACGTG CAC^GTG 2383 CACGTG PmlI 346 CACGTG CAC^GTG 2383 CACGTG PshAI 43 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 332 GACACAGGTC SmaI 1673 CCCGGG CCC^GGG 2922 CCCGGG XmaCI 1673 CCCGGG C^CCGGG 2922 CCCGGG XmaI 1673 CCCGGG C^CCGGG 2922 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI Aor51HI AvaI AvaII BamHI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII