##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AclI AcyI AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspEI AspI AviII AvrII
BbeI BbrPI BcgI BglII BlnI
BsaBI BsaHI BsiEI BsiWI BsmBI
Bsp1407I BspHI BspMI BsrFI BsrGI
BssHII BssSI BstEII BstPI Bsu36I
Cfr10I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HgaI
Hin1I HindIII KasI KspI MamI
MfeI MluI MunI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SpeI SphI
Sse8387I SspBI SwaI Tth111I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 2226 GGTACC G^GTACC
AccII 159 CGCG CG^CG
AccIII 1516 TCCGGA T^CCGGA
AflII 1014 CTTAAG C^TTAAG
AseI 2285 ATTAAT AT^TAAT
AsnI 2285 ATTAAT AT^TAAT
Asp718I 2226 GGTACC G^GTACC
AspHI 2484 GTGCTC G[AT]GC[AT]^C
AvaI 1338 CTCGGG C^[CT]CG[GA]G
BalI 2234 TGGCCA TGG^CCA
BanII 3166 GGGCTC G[GA]GC[CT]^C
BbvCI 1644 GCTGAGG CCTCAGC(-5/-2)
BciVI 1454 GGATAC GTATCC(6/5)
BfrI 1014 CTTAAG C^TTAAG
BglI 565 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3244 TACGTA [CT]AC^GT[GA]
BsaI 3036 GGTCTC GGTCTC(1/5)
BsaWI 1516 TCCGGA [AT]^CCGG[AT]
BseAI 1516 TCCGGA T^CCGGA
BsiHKAI 2484 GTGCTC G[AT]GC[AT]^C
BsoBI 1338 CTCGGG C^[CT]CG[GA]G
BspDI 385 ATCGAT AT^CGAT
BspEI 1516 TCCGGA T^CCGGA
BsrBI 5 CCGCTC CCGCTC(-3/-3)
BsrDI 966 GCAATG GCAATG(2/0)
Bst1107I 3178 GTATAC GTA^TAC
BstBI 2909 TTCGAA TT^CGAA
BstUI 159 CGCG CG^CG
BstXI 2346 CCAGTACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3178 GTATAC GTA^TAC
ClaI 385 ATCGAT AT^CGAT
DrdI 2058 GACAGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2231 CCATGG C^C[GA][CT]GG
EcoRI 1480 GAATTC G^AATTC
FauI 46 GCGGG CCCGC(4/6)
HincII 2973 GTTAAC GT[CT]^[GA]AC
HindII 2973 GTTAAC GT[CT]^[GA]AC
HpaI 2973 GTTAAC GTT^AAC
KpnI 2226 GGTACC GGTAC^C
MluNI 2234 TGGCCA TGG^CCA
MroI 1516 TCCGGA T^CCGGA
MscI 2234 TGGCCA TGG^CCA
MvnI 159 CGCG CG^CG
NcoI 2231 CCATGG C^CATGG
NspV 2909 TTCGAA TT^CGAA
PpuMI 1168 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2285 ATTAAT AT^TAAT
PstI 2770 CTGCAG CTGCA^G
SapI 1113 GAAGAGC GCTCTTC(1/4)
SfuI 2909 TTCGAA TT^CGAA
SnaBI 3244 TACGTA TAC^GTA
SspI 2105 AATATT AAT^ATT
StuI 1502 AGGCCT AGG^CCT
XbaI 2604 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 2134 ACATGT A^C[GA][CT]GT
3220 ACATGT
BamHI 911 GGATCC G^GATCC
1532 GGATCC
BclI 1040 TGATCA T^GATCA
3285 TGATCA
BlpI 134 GCTCAGC GC^T[ACGT]AGC
3168 GCTCAGC
Bpu1102I 134 GCTCAGC GC^T[ACGT]AGC
3168 GCTCAGC
BsgI 261 CTGCAC GTGCAG(16/14)
2246 GTGCAG
BspLU11I 2134 ACATGT A^CATGT
3220 ACATGT
BstAPI 2477 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2772 GCAGCCAGTGC
CelII 134 GCTCAGC GC^T[ACGT]AGC
3168 GCTCAGC
FbaI 1040 TGATCA T^GATCA
3285 TGATCA
HaeII 1718 AGCGCC [GA]GCGC^[CT]
1852 GGCGCT
NspI 2134 ACATGT [GA]CATG^[CT]
3220 ACATGT
SfcI 2029 CTACAG C^T[GA][CT]AG
2770 CTGCAG
SmlI 1014 CTTAAG C^T[CT][GA]AG
1506 CTCAAG
XcmI 477 CCACTAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
560 CCAGGGCCTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI