##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AccII AccIII AclI AflII AflIII
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbeI BfrI
BglI BglII BlnI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BspMI BssHII BssSI Bst1107I
BstBI BstUI BstYI BstZ17I ClaI
DraI DrdI EagI EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KspI MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI SacI SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 168 GACGTC GACGT^C
Acc65I 2746 GGTACC G^GTACC
AccI 1094 GTAGAC GT^[AC][GT]AC
AgeI 2482 ACCGGT A^CCGGT
AhdI 977 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2829 GTGCAC G^TGCAC
ApaLI 2829 GTGCAC G^TGCAC
Asp718I 2746 GGTACC G^GTACC
AspEI 977 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2577 CACGTG CAC^GTG
BcgI 365 GCAGAGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2073 GTATCC GTATCC(6/5)
BclI 2316 TGATCA T^GATCA
BsaWI 2482 ACCGGT [AT]^CCGG[AT]
Bsp1407I 3043 TGTACA T^GTACA
BsrFI 2482 ACCGGT [GA]^CCGG[CT]
BsrGI 3043 TGTACA T^GTACA
BstEII 2959 GGTCACC G^GT[ACGT]ACC
BstPI 2959 GGTCACC G^GT[ACGT]ACC
Cfr10I 2482 ACCGGT [GA]^CCGG[CT]
CpoI 754 CGGACCG CG^G[AT]CCG
DraIII 2251 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 977 GACCATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2959 GGTCACC G^GT[ACGT]ACC
FbaI 2316 TGATCA T^GATCA
KpnI 2746 GGTACC GGTAC^C
NcoI 2097 CCATGG C^CATGG
NdeI 1683 CATATG CA^TATG
NspI 2169 ACATGC [GA]CATG^[CT]
PinAI 2482 ACCGGT A^CCGGT
PmaCI 2577 CACGTG CAC^GTG
PmlI 2577 CACGTG CAC^GTG
RsrII 754 CGGACCG CG^G[AT]CCG
SbfI 692 CCTGCAGG CCTGCA^GG
ScaI 1105 AGTACT AGT^ACT
SmlI 2033 CTCAAG C^T[CT][GA]AG
Sse8387I 692 CCTGCAGG CCTGCA^GG
SspBI 3043 TGTACA T^GTACA
StuI 1645 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 69 AAATTT [GA]^AATT[CT]
1604 GAATTT
AcyI 168 GACGTC G[GA]^CG[CT]C
265 GGCGTC
AlwI 134 GATCC GGATC(4/5)
2227 GGATC
AocI 1004 CCTGAGG CC^T[ACGT]AGG
1358 CCTGAGG
Aor51HI 1979 AGCGCT AGC^GCT
2181 AGCGCT
ApoI 69 AAATTT [GA]^AATT[CT]
1604 GAATTT
BalI 2140 TGGCCA TGG^CCA
2865 TGGCCA
BlpI 1199 GCTGAGC GC^T[ACGT]AGC
2549 GCTCAGC
Bpu1102I 1199 GCTGAGC GC^T[ACGT]AGC
2549 GCTCAGC
BsaAI 2186 TACGTG [CT]AC^GT[GA]
2577 CACGTG
BsaHI 168 GACGTC G[GA]^CG[CT]C
265 GGCGTC
BsaI 1184 GAGACC GGTCTC(1/5)
2851 GGTCTC
BsmBI 984 CGTCTC CGTCTC(1/5)
1891 CGTCTC
BsrBI 1528 CCGCTC CCGCTC(-3/-3)
950 GAGCGG
BsrDI 678 CATTGC GCAATG(2/0)
2107 CATTGC
BstXI 2364 CCATCGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3003 CCAGGATGGTGG
Bsu36I 1004 CCTGAGG CC^T[ACGT]AGG
1358 CCTGAGG
CelII 1199 GCTGAGC GC^T[ACGT]AGC
2549 GCTCAGC
DsaI 2097 CCATGG C^C[GA][CT]GG
2452 CCGTGG
Eco47III 1979 AGCGCT AGC^GCT
2181 AGCGCT
Eco81I 1004 CCTGAGG CC^T[ACGT]AGG
1358 CCTGAGG
Hin1I 168 GACGTC G[GA]^CG[CT]C
265 GGCGTC
MluNI 2140 TGGCCA TGG^CCA
2865 TGGCCA
MscI 2140 TGGCCA TGG^CCA
2865 TGGCCA
SmaI 939 CCCGGG CCC^GGG
1803 CCCGGG
XmaCI 939 CCCGGG C^CCGGG
1803 CCCGGG
XmaI 939 CCCGGG C^CCGGG
1803 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I