##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AocI Aor51HI ApaI AscI Asp718I
AspI BbeI BbrPI BclI BfrI
BsaAI BsaI BseAI BsiWI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DrdI Eco47III Eco81I EcoNI
EcoO65I EcoRI FbaI FseI HpaI
KasI KpnI MluI MroI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
RsrII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 2411 GACGTC GACGT^C
AccI 1715 GTCGAC GT^[AC][GT]AC
AhdI 68 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3087 GTGCAC G^TGCAC
ApaLI 3087 GTGCAC G^TGCAC
AseI 3466 ATTAAT AT^TAAT
AsnI 3466 ATTAAT AT^TAAT
Asp700I 530 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 68 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 948 TGCGCA TGC^GCA
BbvCI 1590 GCTGAGG CCTCAGC(-5/-2)
BcgI 2092 CGAGATAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 340 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 357 GCTGAGC GC^T[ACGT]AGC
BpmI 2812 CTGGAG CTGGAG(16/14)
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
BsaWI 2722 ACCGGA [AT]^CCGG[AT]
BsmBI 2409 GAGACG CGTCTC(1/5)
Bsp1407I 3508 TGTACA T^GTACA
BspLU11I 3117 ACATGT A^CATGT
BsrBI 38 CCGCTC CCGCTC(-3/-3)
BsrFI 10 GCCGGT [GA]^CCGG[CT]
BsrGI 3508 TGTACA T^GTACA
BstAPI 304 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2378 CCAATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 357 GCTGAGC GC^T[ACGT]AGC
Cfr10I 10 GCCGGT [GA]^CCGG[CT]
EagI 8 CGGCCG C^GGCCG
Eam1105I 68 GACGAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
EcoRV 1426 GATATC GAT^ATC
FauI 2183 GCGGG CCCGC(4/6)
FspI 948 TGCGCA TGC^GCA
HaeII 1833 GGCGCT [GA]GCGC^[CT]
KspI 1656 CCGCGG CCGC^GG
PflMI 3750 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3165 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3466 ATTAAT AT^TAAT
PvuI 327 CGATCG CGAT^CG
PvuII 842 CAGCTG CAG^CTG
SacII 1656 CCGCGG CCGC^GG
SalI 1715 GTCGAC G^TCGAC
ScaI 3814 AGTACT AGT^ACT
SmlI 3252 CTTGAG C^T[CT][GA]AG
SpeI 3645 ACTAGT A^CTAGT
SphI 2102 GCATGC GCATG^C
SspBI 3508 TGTACA T^GTACA
Van91I 3750 CCATTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2248 TCTAGA T^CTAGA
XmnI 530 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 3091 ACACGT A^C[GA][CT]GT
3117 ACATGT
AlwNI 305 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
1120 CAGGTTCTG
BalI 1889 TGGCCA TGG^CCA
3199 TGGCCA
BamHI 975 GGATCC G^GATCC
1551 GGATCC
BanI 337 GGTGCC G^G[CT][GA]CC
3922 GGCACC
BciVI 1504 GGATAC GTATCC(6/5)
2268 GGATAC
BglII 1480 AGATCT A^GATCT
2616 AGATCT
BsaBI 1374 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2233 GATGAAGATC
BspMI 1205 ACCTGC ACCTGC(4/8)
1108 GCAGGT
DraIII 1042 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
2462 CACAGTGTG
EarI 853 GAAGAG CTCTTC(1/4)
1861 GAAGAG
HindIII 468 AAGCTT A^AGCTT
514 AAGCTT
Ksp632I 853 GAAGAG CTCTTC(1/4)
1861 GAAGAG
MamI 1374 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2233 GATGAAGATC
MfeI 1520 CAATTG C^AATTG
2369 CAATTG
MluNI 1889 TGGCCA TGG^CCA
3199 TGGCCA
MscI 1889 TGGCCA TGG^CCA
3199 TGGCCA
MunI 1520 CAATTG C^AATTG
2369 CAATTG
NdeI 751 CATATG CA^TATG
3303 CATATG
PpuMI 2664 AGGACCT [GA]G^G[AT]CC[CT]
2790 GGGACCC
PstI 839 CTGCAG CTGCA^G
863 CTGCAG
SmaI 138 CCCGGG CCC^GGG
182 CCCGGG
XcmI 1412 CCACTCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1856 CCAATGAAGAGGTGG
XmaCI 138 CCCGGG C^CCGGG
182 CCCGGG
XmaI 138 CCCGGG C^CCGGG
182 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII AvrII BanII BbsI BbvI
BcnI BfaI BlnI BmyI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII