##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AspEI
AspI AvaI AviII BamHI BbeI
BbvCI BcgI BciVI BclI BglI
BlpI BpmI Bpu1102I BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KspI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 887 GGTACC G^GTACC
AclI 983 AACGTT AA^CGTT
AflIII 1254 ACGTGT A^C[GA][CT]GT
AseI 2821 ATTAAT AT^TAAT
AsnI 2821 ATTAAT AT^TAAT
Asp700I 2251 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 887 GGTACC G^GTACC
AvrII 1812 CCTAGG C^CTAGG
BalI 114 TGGCCA TGG^CCA
BanII 736 GAGCCC G[GA]GC[CT]^C
BbrPI 1253 CACGTG CAC^GTG
BcnI 225 CCGGG CC^[GC]GG
BlnI 1812 CCTAGG C^CTAGG
BsmBI 2687 GAGACG CGTCTC(1/5)
BsrBI 570 CCGCTC CCGCTC(-3/-3)
BstEII 1336 GGTGACC G^GT[ACGT]ACC
BstPI 1336 GGTGACC G^GT[ACGT]ACC
BstXI 932 CCAAATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1734 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 114 TGGCCA [CT]^GGCC[GA]
EcoO65I 1336 GGTGACC G^GT[ACGT]ACC
EcoRI 616 GAATTC G^AATTC
EcoRV 971 GATATC GAT^ATC
HapII 225 CCGG C^CGG
HincII 1469 GTTGAC GT[CT]^[GA]AC
HindII 1469 GTTGAC GT[CT]^[GA]AC
HpaII 225 CCGG C^CGG
KpnI 887 GGTACC GGTAC^C
MfeI 2152 CAATTG C^AATTG
MluNI 114 TGGCCA TGG^CCA
MscI 114 TGGCCA TGG^CCA
MspA1I 874 CAGCTG C[AC]G^C[GT]G
MspI 225 CCGG C^CGG
MunI 2152 CAATTG C^AATTG
NciI 225 CCGGG CC^[GC]GG
PmaCI 1253 CACGTG CAC^GTG
PmlI 1253 CACGTG CAC^GTG
PshBI 2821 ATTAAT AT^TAAT
Psp1406I 983 AACGTT AA^CGTT
PstI 2092 CTGCAG CTGCA^G
PvuII 874 CAGCTG CAG^CTG
ScaI 1379 AGTACT AGT^ACT
SphI 169 GCATGC GCATG^C
XbaI 1459 TCTAGA T^CTAGA
XmnI 2251 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 570 CCGC CCGC(-3/-1)
885 GCGG
AlwI 328 GATCC GGATC(4/5)
2578 GATCC
AspHI 1412 GTGCTC G[AT]GC[AT]^C
1712 GTGCTC
BanI 359 GGCACC G^G[CT][GA]CC
887 GGTACC
BglII 51 AGATCT A^GATCT
2645 AGATCT
BsaAI 1253 CACGTG [CT]AC^GT[GA]
2638 TACGTG
BsaBI 782 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1656 GATTCCCATC
BsgI 1874 CTGCAC GTGCAG(16/14)
2641 GTGCAG
BsiHKAI 1412 GTGCTC G[AT]GC[AT]^C
1712 GTGCTC
BsrDI 200 GCAATG GCAATG(2/0)
1958 GCAATG
DraII 1437 GGGACCT [GA]G^G[ACGT]CC[CT]
2740 GGGACCT
EcoO109I 1437 GGGACCT [GA]G^G[ACGT]CC[CT]
2740 GGGACCT
EcoT22I 1173 ATGCAT ATGCA^T
1542 ATGCAT
HindIII 2367 AAGCTT A^AGCTT
2679 AAGCTT
MamI 782 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1656 GATTCCCATC
MslI 170 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
256 CACGACAGTG
NcoI 791 CCATGG C^CATGG
1107 CCATGG
NsiI 1173 ATGCAT ATGCA^T
1542 ATGCAT
PpuMI 1437 GGGACCT [GA]G^G[AT]CC[CT]
2740 GGGACCT
TaqI 71 TCGA T^CGA
542 TCGA
TthHB8I 71 TCGA T^CGA
542 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AflII AluI ApoI
AvaII BbsI BbvI BfaI BfrI
BmyI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII