################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AgeI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII BamHI BbeI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrDI BsrFI BssHII BstBI BstEII BstPI BstUI BstXI Cfr10I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflII 174 CTTAAG C^TTAAG Alw44I 2921 GTGCAC G^TGCAC AocI 453 CCTCAGG CC^T[ACGT]AGG ApaLI 2921 GTGCAC G^TGCAC AvrII 575 CCTAGG C^CTAGG BbrPI 780 CACGTG CAC^GTG BbsI 1331 GTCTTC GAAGAC(2/6) BbvCI 2244 GCTGAGG CCTCAGC(-5/-2) BcgI 2406 CGAAGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1497 GGATAC GTATCC(6/5) BclI 480 TGATCA T^GATCA BfrI 174 CTTAAG C^TTAAG BglI 1388 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 575 CCTAGG C^CTAGG BpuAI 1331 GTCTTC GAAGAC(2/6) BsaAI 780 CACGTG [CT]AC^GT[GA] BsaBI 2000 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 777 CTGCAC GTGCAG(16/14) Bsp1407I 806 TGTACA T^GTACA BsrBI 1440 CCGCTC CCGCTC(-3/-3) BsrGI 806 TGTACA T^GTACA Bst1107I 919 GTATAC GTA^TAC BstAPI 2737 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 919 GTATAC GTA^TAC Bsu36I 453 CCTCAGG CC^T[ACGT]AGG DraIII 848 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 826 GACATACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 453 CCTCAGG CC^T[ACGT]AGG FbaI 480 TGATCA T^GATCA HaeII 2487 AGCGCC [GA]GCGC^[CT] HgaI 1832 GCGTC GACGC(5/10) HincII 523 GTTGAC GT[CT]^[GA]AC HindII 523 GTTGAC GT[CT]^[GA]AC MaeII 781 ACGT A^CGT MamI 2000 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PmaCI 780 CACGTG CAC^GTG PmlI 780 CACGTG CAC^GTG PshAI 179 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2255 GAGCTC GAGCT^C SmaI 2335 CCCGGG CCC^GGG SmlI 174 CTTAAG C^T[CT][GA]AG SspBI 806 TGTACA T^GTACA TaqI 2011 TCGA T^CGA Tsp509I 956 AATT ^AATT TthHB8I 2011 TCGA T^CGA XbaI 530 TCTAGA T^CTAGA XmaCI 2335 CCCGGG C^CCGGG XmaI 2335 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 919 GTATAC GT^[AC][GT]AC 2166 GTCTAC AhdI 1551 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2506 GACAGCCAGTC ApaI 1417 GGGCCC GGGCC^C 2561 GGGCCC AspEI 1551 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2506 GACAGCCAGTC AvaI 2199 CCCGAG C^[CT]CG[GA]G 2335 CCCGGG BalI 763 TGGCCA TGG^CCA 2475 TGGCCA BglII 1188 AGATCT A^GATCT 2621 AGATCT BsaI 938 GAGACC GGTCTC(1/5) 834 GGTCTC BsmI 1875 GAATGC GAATGC(1/-1) 2144 GCATTC BsoBI 2199 CCCGAG C^[CT]CG[GA]G 2335 CCCGGG BssSI 1575 CACGAG CACGAG(-5/-1) 105 CTCGTG CfoI 1672 GCGC GCG^C 2488 GCGC DsaI 1923 CCGTGG C^C[GA][CT]GG 2732 CCACGG EaeI 763 TGGCCA [CT]^GGCC[GA] 2475 TGGCCA Eam1105I 1551 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2506 GACAGCCAGTC EarI 1443 CTCTTC CTCTTC(1/4) 2139 GAAGAG FauI 1439 CCCGC CCCGC(4/6) 2635 GCGGG HhaI 1672 GCGC GCG^C 2488 GCGC HinP1I 1672 GCGC G^CGC 2488 GCGC HindIII 55 AAGCTT A^AGCTT 93 AAGCTT Ksp632I 1443 CTCTTC CTCTTC(1/4) 2139 GAAGAG MluNI 763 TGGCCA TGG^CCA 2475 TGGCCA MscI 763 TGGCCA TGG^CCA 2475 TGGCCA MslI 1591 CATGTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2817 CATCACAGTG MspA1I 1194 CAGCTG C[AC]G^C[GT]G 1284 CAGCTG NheI 990 GCTAGC G^CTAGC 1460 GCTAGC PstI 2573 CTGCAG CTGCA^G 2759 CTGCAG PvuII 1194 CAGCTG CAG^CTG 1284 CAGCTG SapI 2139 GAAGAGC GCTCTTC(1/4) 1442 GCTCTTC StuI 1395 AGGCCT AGG^CCT 2545 AGGCCT XcmI 1039 CCAAGTCTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2492 CCAGGGCAGGCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaII BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraII EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I TspRI Van91I XhoII