##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AgeI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BamHI BbeI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrDI BsrFI
BssHII BstBI BstEII BstPI BstUI
BstXI Cfr10I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 174 CTTAAG C^TTAAG
Alw44I 2921 GTGCAC G^TGCAC
AocI 453 CCTCAGG CC^T[ACGT]AGG
ApaLI 2921 GTGCAC G^TGCAC
AvrII 575 CCTAGG C^CTAGG
BbrPI 780 CACGTG CAC^GTG
BbsI 1331 GTCTTC GAAGAC(2/6)
BbvCI 2244 GCTGAGG CCTCAGC(-5/-2)
BcgI 2406 CGAAGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1497 GGATAC GTATCC(6/5)
BclI 480 TGATCA T^GATCA
BfrI 174 CTTAAG C^TTAAG
BglI 1388 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 575 CCTAGG C^CTAGG
BpuAI 1331 GTCTTC GAAGAC(2/6)
BsaAI 780 CACGTG [CT]AC^GT[GA]
BsaBI 2000 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 777 CTGCAC GTGCAG(16/14)
Bsp1407I 806 TGTACA T^GTACA
BsrBI 1440 CCGCTC CCGCTC(-3/-3)
BsrGI 806 TGTACA T^GTACA
Bst1107I 919 GTATAC GTA^TAC
BstAPI 2737 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 919 GTATAC GTA^TAC
Bsu36I 453 CCTCAGG CC^T[ACGT]AGG
DraIII 848 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 826 GACATACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 453 CCTCAGG CC^T[ACGT]AGG
FbaI 480 TGATCA T^GATCA
HaeII 2487 AGCGCC [GA]GCGC^[CT]
HgaI 1832 GCGTC GACGC(5/10)
HincII 523 GTTGAC GT[CT]^[GA]AC
HindII 523 GTTGAC GT[CT]^[GA]AC
MaeII 781 ACGT A^CGT
MamI 2000 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PmaCI 780 CACGTG CAC^GTG
PmlI 780 CACGTG CAC^GTG
PshAI 179 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2255 GAGCTC GAGCT^C
SmaI 2335 CCCGGG CCC^GGG
SmlI 174 CTTAAG C^T[CT][GA]AG
SspBI 806 TGTACA T^GTACA
TaqI 2011 TCGA T^CGA
Tsp509I 956 AATT ^AATT
TthHB8I 2011 TCGA T^CGA
XbaI 530 TCTAGA T^CTAGA
XmaCI 2335 CCCGGG C^CCGGG
XmaI 2335 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 919 GTATAC GT^[AC][GT]AC
2166 GTCTAC
AhdI 1551 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2506 GACAGCCAGTC
ApaI 1417 GGGCCC GGGCC^C
2561 GGGCCC
AspEI 1551 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2506 GACAGCCAGTC
AvaI 2199 CCCGAG C^[CT]CG[GA]G
2335 CCCGGG
BalI 763 TGGCCA TGG^CCA
2475 TGGCCA
BglII 1188 AGATCT A^GATCT
2621 AGATCT
BsaI 938 GAGACC GGTCTC(1/5)
834 GGTCTC
BsmI 1875 GAATGC GAATGC(1/-1)
2144 GCATTC
BsoBI 2199 CCCGAG C^[CT]CG[GA]G
2335 CCCGGG
BssSI 1575 CACGAG CACGAG(-5/-1)
105 CTCGTG
CfoI 1672 GCGC GCG^C
2488 GCGC
DsaI 1923 CCGTGG C^C[GA][CT]GG
2732 CCACGG
EaeI 763 TGGCCA [CT]^GGCC[GA]
2475 TGGCCA
Eam1105I 1551 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2506 GACAGCCAGTC
EarI 1443 CTCTTC CTCTTC(1/4)
2139 GAAGAG
FauI 1439 CCCGC CCCGC(4/6)
2635 GCGGG
HhaI 1672 GCGC GCG^C
2488 GCGC
HinP1I 1672 GCGC G^CGC
2488 GCGC
HindIII 55 AAGCTT A^AGCTT
93 AAGCTT
Ksp632I 1443 CTCTTC CTCTTC(1/4)
2139 GAAGAG
MluNI 763 TGGCCA TGG^CCA
2475 TGGCCA
MscI 763 TGGCCA TGG^CCA
2475 TGGCCA
MslI 1591 CATGTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2817 CATCACAGTG
MspA1I 1194 CAGCTG C[AC]G^C[GT]G
1284 CAGCTG
NheI 990 GCTAGC G^CTAGC
1460 GCTAGC
PstI 2573 CTGCAG CTGCA^G
2759 CTGCAG
PvuII 1194 CAGCTG CAG^CTG
1284 CAGCTG
SapI 2139 GAAGAGC GCTCTTC(1/4)
1442 GCTCTTC
StuI 1395 AGGCCT AGG^CCT
2545 AGGCCT
XcmI 1039 CCAAGTCTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2492 CCAGGGCAGGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraII EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I TspRI
Van91I XhoII