##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AclI AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI BamHI BbeI
BbsI BciVI BglII BpuAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I ClaI CpoI
DraI Eco47III EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KasI
KpnI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2323 GACGTC GACGT^C
AccIII 2319 TCCGGA T^CCGGA
AflII 299 CTTAAG C^TTAAG
AocI 469 CCTCAGG CC^T[ACGT]AGG
AvrII 1843 CCTAGG C^CTAGG
BalI 900 TGGCCA TGG^CCA
BbvCI 636 CCTCAGC CCTCAGC(-5/-2)
BcgI 68 CGAAGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 411 TGATCA T^GATCA
BfrI 299 CTTAAG C^TTAAG
BglI 2805 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1843 CCTAGG C^CTAGG
BlpI 2087 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2087 GCTCAGC GC^T[ACGT]AGC
BsaWI 2319 TCCGGA [AT]^CCGG[AT]
BseAI 2319 TCCGGA T^CCGGA
BsiEI 285 CGGCCG CG[GA][CT]^CG
BspEI 2319 TCCGGA T^CCGGA
BspMI 1029 ACCTGC ACCTGC(4/8)
BsrFI 1477 GCCGGT [GA]^CCGG[CT]
Bsu36I 469 CCTCAGG CC^T[ACGT]AGG
CelII 2087 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1477 GCCGGT [GA]^CCGG[CT]
DraIII 2296 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2594 GACCAGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 285 CGGCCG C^GGCCG
EclXI 285 CGGCCG C^GGCCG
Eco52I 285 CGGCCG C^GGCCG
Eco81I 469 CCTCAGG CC^T[ACGT]AGG
EcoNI 2174 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 411 TGATCA T^GATCA
HindIII 536 AAGCTT A^AGCTT
KspI 2288 CCGCGG CCGC^GG
MluNI 900 TGGCCA TGG^CCA
MroI 2319 TCCGGA T^CCGGA
MscI 900 TGGCCA TGG^CCA
NcoI 2055 CCATGG C^CATGG
NspI 125 GCATGT [GA]CATG^[CT]
PflMI 1042 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1052 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2288 CCGCGG CCGC^GG
SapI 1483 GAAGAGC GCTCTTC(1/4)
ScaI 660 AGTACT AGT^ACT
Van91I 1042 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2266 TCTAGA T^CTAGA
XcmI 1207 CCACAACCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1001 AAATTC [GA]^AATT[CT]
1020 AAATTC
AcyI 209 GACGCC G[GA]^CG[CT]C
2323 GACGTC
AhdI 341 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2069 GACCCAGAGTC
ApoI 1001 AAATTC [GA]^AATT[CT]
1020 AAATTC
Asp700I 1483 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2258 GAACTGCTTC
AspEI 341 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2069 GACCCAGAGTC
AviII 744 TGCGCA TGC^GCA
828 TGCGCA
BanI 632 GGTGCC G^G[CT][GA]CC
805 GGCACC
BbrPI 1043 CACGTG CAC^GTG
1303 CACGTG
BsaAI 1043 CACGTG [CT]AC^GT[GA]
1303 CACGTG
BsaBI 166 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1609 GATCACCATC
BsaHI 209 GACGCC G[GA]^CG[CT]C
2323 GACGTC
BsmBI 104 GAGACG CGTCTC(1/5)
1496 GAGACG
Eam1105I 341 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2069 GACCCAGAGTC
FspI 744 TGCGCA TGC^GCA
828 TGCGCA
HaeII 51 GGCGCT [GA]GCGC^[CT]
1405 GGCGCT
Hin1I 209 GACGCC G[GA]^CG[CT]C
2323 GACGTC
MamI 166 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1609 GATCACCATC
MseI 300 TTAA T^TAA
576 TTAA
MslI 1141 CACGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2477 CATATTTATG
PmaCI 1043 CACGTG CAC^GTG
1303 CACGTG
PmlI 1043 CACGTG CAC^GTG
1303 CACGTG
SacI 1798 GAGCTC GAGCT^C
2541 GAGCTC
Tru9I 300 TTAA T^TAA
576 TTAA
Tsp509I 1002 AATT ^AATT
1021 AATT
XmnI 1483 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2258 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII XmaCI
XmaI