################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AspI BfrI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV HincII HindII HindIII HpaI KpnI KspI MamI MluI MroI MvnI NdeI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Aor51HI 2917 AGCGCT AGC^GCT AviII 546 TGCGCA TGC^GCA AvrII 1129 CCTAGG C^CTAGG BamHI 2543 GGATCC G^GATCC BbeI 1735 GGCGCC GGCGC^C BbrPI 2199 CACGTG CAC^GTG BbvCI 1253 GCTGAGG CCTCAGC(-5/-2) BcgI 225 GCATAGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 565 GGATAC GTATCC(6/5) BlnI 1129 CCTAGG C^CTAGG BsaAI 2199 CACGTG [CT]AC^GT[GA] BsaI 2582 GAGACC GGTCTC(1/5) BsmBI 578 GAGACG CGTCTC(1/5) BspLU11I 2276 ACATGT A^CATGT BsrFI 2035 GCCGGC [GA]^CCGG[CT] BstAPI 2153 GCAGTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 322 GGTCACC G^GT[ACGT]ACC BstPI 322 GGTCACC G^GT[ACGT]ACC Cfr10I 2035 GCCGGC [GA]^CCGG[CT] DrdI 622 GACAAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2917 AGCGCT AGC^GCT EcoNI 2534 CCTGTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 322 GGTCACC G^GT[ACGT]ACC FseI 2034 GGCCGGCC GGCCGG^CC FspI 546 TGCGCA TGC^GCA KasI 1735 GGCGCC G^GCGCC MfeI 1576 CAATTG C^AATTG MseI 3027 TTAA T^TAA MunI 1576 CAATTG C^AATTG NaeI 2035 GCCGGC GCC^GGC NarI 1735 GGCGCC GG^CGCC NgoMIV 2035 GCCGGC G^CCGGC NheI 1750 GCTAGC G^CTAGC PflMI 2852 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2199 CACGTG CAC^GTG PmlI 2199 CACGTG CAC^GTG SacI 532 GAGCTC GAGCT^C SfoI 1735 GGCGCC GGC^GCC SphI 1909 GCATGC GCATG^C Tru9I 3027 TTAA T^TAA Van91I 2852 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1023 CCATCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 500 GTAGAC GT^[AC][GT]AC 689 GTCTAC AcyI 1735 GGCGCC G[GA]^CG[CT]C 2997 GGCGTC Alw44I 2210 GTGCAC G^TGCAC 2288 GTGCAC ApaLI 2210 GTGCAC G^TGCAC 2288 GTGCAC Asp700I 934 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1833 GAAGTGCTTC BalI 1247 TGGCCA TGG^CCA 1864 TGGCCA BbsI 1284 GTCTTC GAAGAC(2/6) 1956 GTCTTC BclI 972 TGATCA T^GATCA 1449 TGATCA BglI 1727 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2797 GCCTGATGGGC BglII 162 AGATCT A^GATCT 1790 AGATCT BpmI 1654 CTGGAG CTGGAG(16/14) 2104 CTGGAG BpuAI 1284 GTCTTC GAAGAC(2/6) 1956 GTCTTC BsaHI 1735 GGCGCC G[GA]^CG[CT]C 2997 GGCGTC Bsp1407I 1163 TGTACA T^GTACA 1969 TGTACA BsrDI 2338 CATTGC GCAATG(2/0) 1074 GCAATG BsrGI 1163 TGTACA T^GTACA 1969 TGTACA BssSI 480 CACGAG CACGAG(-5/-1) 2168 CACGAG BstXI 2616 CCAACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2640 CCAGCCCCTTGG EarI 1585 GAAGAG CTCTTC(1/4) 1599 GAAGAG FauI 6 CCCGC CCCGC(4/6) 1732 GCGGG FbaI 972 TGATCA T^GATCA 1449 TGATCA HgaI 2744 GCGTC GACGC(5/10) 2998 GCGTC Hin1I 1735 GGCGCC G[GA]^CG[CT]C 2997 GGCGTC Ksp632I 1585 GAAGAG CTCTTC(1/4) 1599 GAAGAG MaeII 543 ACGT A^CGT 2200 ACGT MluNI 1247 TGGCCA TGG^CCA 1864 TGGCCA MscI 1247 TGGCCA TGG^CCA 1864 TGGCCA PaeR7I 102 CTCGAG C^TCGAG 2909 CTCGAG PshAI 2197 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2275 GACATGTGTC SspBI 1163 TGTACA T^GTACA 1969 TGTACA XhoI 102 CTCGAG C^TCGAG 2909 CTCGAG XmnI 934 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1833 GAAGTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BlpI BmyI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eco81I EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII