##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI ApaI AscI
AseI AsnI Asp718I AspEI AspI
BfrI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrBI BssHII
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV HincII HindII
HindIII HpaI KpnI KspI MamI
MluI MroI MvnI NdeI NotI
NruI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Aor51HI 2917 AGCGCT AGC^GCT
AviII 546 TGCGCA TGC^GCA
AvrII 1129 CCTAGG C^CTAGG
BamHI 2543 GGATCC G^GATCC
BbeI 1735 GGCGCC GGCGC^C
BbrPI 2199 CACGTG CAC^GTG
BbvCI 1253 GCTGAGG CCTCAGC(-5/-2)
BcgI 225 GCATAGCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 565 GGATAC GTATCC(6/5)
BlnI 1129 CCTAGG C^CTAGG
BsaAI 2199 CACGTG [CT]AC^GT[GA]
BsaI 2582 GAGACC GGTCTC(1/5)
BsmBI 578 GAGACG CGTCTC(1/5)
BspLU11I 2276 ACATGT A^CATGT
BsrFI 2035 GCCGGC [GA]^CCGG[CT]
BstAPI 2153 GCAGTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 322 GGTCACC G^GT[ACGT]ACC
BstPI 322 GGTCACC G^GT[ACGT]ACC
Cfr10I 2035 GCCGGC [GA]^CCGG[CT]
DrdI 622 GACAAGTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2917 AGCGCT AGC^GCT
EcoNI 2534 CCTGTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 322 GGTCACC G^GT[ACGT]ACC
FseI 2034 GGCCGGCC GGCCGG^CC
FspI 546 TGCGCA TGC^GCA
KasI 1735 GGCGCC G^GCGCC
MfeI 1576 CAATTG C^AATTG
MseI 3027 TTAA T^TAA
MunI 1576 CAATTG C^AATTG
NaeI 2035 GCCGGC GCC^GGC
NarI 1735 GGCGCC GG^CGCC
NgoMIV 2035 GCCGGC G^CCGGC
NheI 1750 GCTAGC G^CTAGC
PflMI 2852 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2199 CACGTG CAC^GTG
PmlI 2199 CACGTG CAC^GTG
SacI 532 GAGCTC GAGCT^C
SfoI 1735 GGCGCC GGC^GCC
SphI 1909 GCATGC GCATG^C
Tru9I 3027 TTAA T^TAA
Van91I 2852 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1023 CCATCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 500 GTAGAC GT^[AC][GT]AC
689 GTCTAC
AcyI 1735 GGCGCC G[GA]^CG[CT]C
2997 GGCGTC
Alw44I 2210 GTGCAC G^TGCAC
2288 GTGCAC
ApaLI 2210 GTGCAC G^TGCAC
2288 GTGCAC
Asp700I 934 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1833 GAAGTGCTTC
BalI 1247 TGGCCA TGG^CCA
1864 TGGCCA
BbsI 1284 GTCTTC GAAGAC(2/6)
1956 GTCTTC
BclI 972 TGATCA T^GATCA
1449 TGATCA
BglI 1727 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2797 GCCTGATGGGC
BglII 162 AGATCT A^GATCT
1790 AGATCT
BpmI 1654 CTGGAG CTGGAG(16/14)
2104 CTGGAG
BpuAI 1284 GTCTTC GAAGAC(2/6)
1956 GTCTTC
BsaHI 1735 GGCGCC G[GA]^CG[CT]C
2997 GGCGTC
Bsp1407I 1163 TGTACA T^GTACA
1969 TGTACA
BsrDI 2338 CATTGC GCAATG(2/0)
1074 GCAATG
BsrGI 1163 TGTACA T^GTACA
1969 TGTACA
BssSI 480 CACGAG CACGAG(-5/-1)
2168 CACGAG
BstXI 2616 CCAACCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2640 CCAGCCCCTTGG
EarI 1585 GAAGAG CTCTTC(1/4)
1599 GAAGAG
FauI 6 CCCGC CCCGC(4/6)
1732 GCGGG
FbaI 972 TGATCA T^GATCA
1449 TGATCA
HgaI 2744 GCGTC GACGC(5/10)
2998 GCGTC
Hin1I 1735 GGCGCC G[GA]^CG[CT]C
2997 GGCGTC
Ksp632I 1585 GAAGAG CTCTTC(1/4)
1599 GAAGAG
MaeII 543 ACGT A^CGT
2200 ACGT
MluNI 1247 TGGCCA TGG^CCA
1864 TGGCCA
MscI 1247 TGGCCA TGG^CCA
1864 TGGCCA
PaeR7I 102 CTCGAG C^TCGAG
2909 CTCGAG
PshAI 2197 GACACGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2275 GACATGTGTC
SspBI 1163 TGTACA T^GTACA
1969 TGTACA
XhoI 102 CTCGAG C^TCGAG
2909 CTCGAG
XmnI 934 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1833 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BlpI BmyI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII