################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Alw44I ApaLI AscI Asp718I AviII BbrPI BbvCI BciVI BfrI BglII BsaBI BsaWI BseAI BsiWI BsmI BspEI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraIII EagI EclXI Eco52I EcoO65I EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AflIII 3000 ACATGT A^C[GA][CT]GT AhdI 1960 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2582 CCTGAGG CC^T[ACGT]AGG AseI 1336 ATTAAT AT^TAAT AsnI 1336 ATTAAT AT^TAAT Asp700I 1467 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1960 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1846 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 3195 CCTAGG C^CTAGG BalI 2012 TGGCCA TGG^CCA BamHI 1227 GGATCC G^GATCC BbeI 1051 GGCGCC GGCGC^C BclI 1310 TGATCA T^GATCA BlnI 3195 CCTAGG C^CTAGG BlpI 1677 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1677 GCTGAGC GC^T[ACGT]AGC BsaAI 3174 CACGTA [CT]AC^GT[GA] BsiEI 2409 CGACCG CG[GA][CT]^CG Bsp1407I 3082 TGTACA T^GTACA BspDI 2353 ATCGAT AT^CGAT BspLU11I 3000 ACATGT A^CATGT BspMI 2447 ACCTGC ACCTGC(4/8) BsrDI 1292 CATTGC GCAATG(2/0) BsrGI 3082 TGTACA T^GTACA BstAPI 2600 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 614 CCACCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2582 CCTGAGG CC^T[ACGT]AGG CelII 1677 GCTGAGC GC^T[ACGT]AGC ClaI 2353 ATCGAT AT^CGAT DraI 2236 TTTAAA TTT^AAA DrdI 2359 GACCAAACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1960 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2582 CCTGAGG CC^T[ACGT]AGG EcoNI 2638 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 127 GAATTC G^AATTC EcoRV 1534 GATATC GAT^ATC FbaI 1310 TGATCA T^GATCA HincII 601 GTCAAC GT[CT]^[GA]AC HindII 601 GTCAAC GT[CT]^[GA]AC KasI 1051 GGCGCC G^GCGCC KspI 2989 CCGCGG CCGC^GG MluNI 2012 TGGCCA TGG^CCA MscI 2012 TGGCCA TGG^CCA NarI 1051 GGCGCC GG^CGCC PflFI 1846 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1809 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 1336 ATTAAT AT^TAAT PvuII 857 CAGCTG CAG^CTG SacI 166 GAGCTC GAGCT^C SacII 2989 CCGCGG CCGC^GG SapI 155 GCTCTTC GCTCTTC(1/4) SbfI 1347 CCTGCAGG CCTGCA^GG SexAI 2387 ACCAGGT A^CC[AT]GGT SfiI 72 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1051 GGCGCC GGC^GCC Sse8387I 1347 CCTGCAGG CCTGCA^GG SspBI 3082 TGTACA T^GTACA Tth111I 1846 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 1467 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Aor51HI 2120 AGCGCT AGC^GCT 2290 AGCGCT ApaI 1732 GGGCCC GGGCC^C 2587 GGGCCC BglI 73 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 952 GCCAACGGGGC BsaI 397 GAGACC GGTCTC(1/5) 469 GAGACC BsmBI 25 GAGACG CGTCTC(1/5) 66 GAGACG BspHI 1098 TCATGA T^CATGA 1463 TCATGA BsrBI 153 CCGCTC CCGCTC(-3/-3) 630 GAGCGG BsrFI 493 GCCGGC [GA]^CCGG[CT] 1616 GCCGGC BstYI 371 AGATCC [GA]^GATC[CT] 1227 GGATCC Cfr10I 493 GCCGGC [GA]^CCGG[CT] 1616 GCCGGC DraII 1809 GGGTCCC [GA]G^G[ACGT]CC[CT] 2586 AGGGCCC EarI 156 CTCTTC CTCTTC(1/4) 1669 GAAGAG Eco47III 2120 AGCGCT AGC^GCT 2290 AGCGCT EcoO109I 1809 GGGTCCC [GA]G^G[ACGT]CC[CT] 2586 AGGGCCC Ksp632I 156 CTCTTC CTCTTC(1/4) 1669 GAAGAG MflI 371 AGATCC [GA]^GATC[CT] 1227 GGATCC NaeI 493 GCCGGC GCC^GGC 1616 GCCGGC NgoMIV 493 GCCGGC G^CCGGC 1616 GCCGGC NspI 1481 ACATGC [GA]CATG^[CT] 3000 ACATGT PflMI 175 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2151 CCAGAAGCTGG RcaI 1098 TCATGA T^CATGA 1463 TCATGA SmaI 933 CCCGGG CCC^GGG 2686 CCCGGG Van91I 175 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2151 CCAGAAGCTGG XcmI 1127 CCATGGCGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2520 CCACCTCTCTAGTGG XhoII 371 AGATCC [GA]^GATC[CT] 1227 GGATCC XmaCI 933 CCCGGG C^CCGGG 2686 CCCGGG XmaI 933 CCCGGG C^CCGGG 2686 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AatII AccI AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I