##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaLI AscI Asp718I AviII
BbrPI BbvCI BciVI BfrI BglII
BsaBI BsaWI BseAI BsiWI BsmI
BspEI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraIII EagI
EclXI Eco52I EcoO65I EcoT22I FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI ScaI SfuI SgrAI SmlI
SnaBI SpeI SphI SspI StuI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AflIII 3000 ACATGT A^C[GA][CT]GT
AhdI 1960 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2582 CCTGAGG CC^T[ACGT]AGG
AseI 1336 ATTAAT AT^TAAT
AsnI 1336 ATTAAT AT^TAAT
Asp700I 1467 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1960 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1846 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3195 CCTAGG C^CTAGG
BalI 2012 TGGCCA TGG^CCA
BamHI 1227 GGATCC G^GATCC
BbeI 1051 GGCGCC GGCGC^C
BclI 1310 TGATCA T^GATCA
BlnI 3195 CCTAGG C^CTAGG
BlpI 1677 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1677 GCTGAGC GC^T[ACGT]AGC
BsaAI 3174 CACGTA [CT]AC^GT[GA]
BsiEI 2409 CGACCG CG[GA][CT]^CG
Bsp1407I 3082 TGTACA T^GTACA
BspDI 2353 ATCGAT AT^CGAT
BspLU11I 3000 ACATGT A^CATGT
BspMI 2447 ACCTGC ACCTGC(4/8)
BsrDI 1292 CATTGC GCAATG(2/0)
BsrGI 3082 TGTACA T^GTACA
BstAPI 2600 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 614 CCACCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2582 CCTGAGG CC^T[ACGT]AGG
CelII 1677 GCTGAGC GC^T[ACGT]AGC
ClaI 2353 ATCGAT AT^CGAT
DraI 2236 TTTAAA TTT^AAA
DrdI 2359 GACCAAACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1960 GACATCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2582 CCTGAGG CC^T[ACGT]AGG
EcoNI 2638 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 127 GAATTC G^AATTC
EcoRV 1534 GATATC GAT^ATC
FbaI 1310 TGATCA T^GATCA
HincII 601 GTCAAC GT[CT]^[GA]AC
HindII 601 GTCAAC GT[CT]^[GA]AC
KasI 1051 GGCGCC G^GCGCC
KspI 2989 CCGCGG CCGC^GG
MluNI 2012 TGGCCA TGG^CCA
MscI 2012 TGGCCA TGG^CCA
NarI 1051 GGCGCC GG^CGCC
PflFI 1846 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1809 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1336 ATTAAT AT^TAAT
PvuII 857 CAGCTG CAG^CTG
SacI 166 GAGCTC GAGCT^C
SacII 2989 CCGCGG CCGC^GG
SapI 155 GCTCTTC GCTCTTC(1/4)
SbfI 1347 CCTGCAGG CCTGCA^GG
SexAI 2387 ACCAGGT A^CC[AT]GGT
SfiI 72 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1051 GGCGCC GGC^GCC
Sse8387I 1347 CCTGCAGG CCTGCA^GG
SspBI 3082 TGTACA T^GTACA
Tth111I 1846 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1467 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Aor51HI 2120 AGCGCT AGC^GCT
2290 AGCGCT
ApaI 1732 GGGCCC GGGCC^C
2587 GGGCCC
BglI 73 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
952 GCCAACGGGGC
BsaI 397 GAGACC GGTCTC(1/5)
469 GAGACC
BsmBI 25 GAGACG CGTCTC(1/5)
66 GAGACG
BspHI 1098 TCATGA T^CATGA
1463 TCATGA
BsrBI 153 CCGCTC CCGCTC(-3/-3)
630 GAGCGG
BsrFI 493 GCCGGC [GA]^CCGG[CT]
1616 GCCGGC
BstYI 371 AGATCC [GA]^GATC[CT]
1227 GGATCC
Cfr10I 493 GCCGGC [GA]^CCGG[CT]
1616 GCCGGC
DraII 1809 GGGTCCC [GA]G^G[ACGT]CC[CT]
2586 AGGGCCC
EarI 156 CTCTTC CTCTTC(1/4)
1669 GAAGAG
Eco47III 2120 AGCGCT AGC^GCT
2290 AGCGCT
EcoO109I 1809 GGGTCCC [GA]G^G[ACGT]CC[CT]
2586 AGGGCCC
Ksp632I 156 CTCTTC CTCTTC(1/4)
1669 GAAGAG
MflI 371 AGATCC [GA]^GATC[CT]
1227 GGATCC
NaeI 493 GCCGGC GCC^GGC
1616 GCCGGC
NgoMIV 493 GCCGGC G^CCGGC
1616 GCCGGC
NspI 1481 ACATGC [GA]CATG^[CT]
3000 ACATGT
PflMI 175 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2151 CCAGAAGCTGG
RcaI 1098 TCATGA T^CATGA
1463 TCATGA
SmaI 933 CCCGGG CCC^GGG
2686 CCCGGG
Van91I 175 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2151 CCAGAAGCTGG
XcmI 1127 CCATGGCGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2520 CCACCTCTCTAGTGG
XhoII 371 AGATCC [GA]^GATC[CT]
1227 GGATCC
XmaCI 933 CCCGGG C^CCGGG
2686 CCCGGG
XmaI 933 CCCGGG C^CCGGG
2686 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BssSI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I