##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BalI BbeI BbrPI BcgI BfrI
BglI BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI Bst1107I BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FspI HaeII Hin1I HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI NarI NcoI NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 248 GTCTAC GT^[AC][GT]AC
AhdI 840 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 438 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 840 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2464 GTGCTC G[AT]GC[AT]^C
BamHI 1076 GGATCC G^GATCC
BanI 1025 GGTGCC G^G[CT][GA]CC
BciVI 1635 GTATCC GTATCC(6/5)
BclI 1331 TGATCA T^GATCA
BcnI 126 CCGGG CC^[GC]GG
BglII 500 AGATCT A^GATCT
BsaAI 2361 TACGTG [CT]AC^GT[GA]
BsaWI 1292 TCCGGT [AT]^CCGG[AT]
BsiHKAI 2464 GTGCTC G[AT]GC[AT]^C
BsmI 2888 GAATGC GAATGC(1/-1)
BspHI 1111 TCATGA T^CATGA
BsrFI 144 GCCGGC [GA]^CCGG[CT]
BssSI 1763 CACGAG CACGAG(-5/-1)
BstAPI 2665 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 574 TTCGAA TT^CGAA
Cfr10I 144 GCCGGC [GA]^CCGG[CT]
DraI 2940 TTTAAA TTT^AAA
DsaI 1554 CCACGG C^C[GA][CT]GG
Eam1105I 840 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1067 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 896 GAATTC G^AATTC
EcoT14I 463 CCAAGG C^C[AT][AT]GG
FbaI 1331 TGATCA T^GATCA
FseI 143 GGCCGGCC GGCCGG^CC
HgaI 46 GACGC GACGC(5/10)
HincII 2412 GTCAAC GT[CT]^[GA]AC
HindII 2412 GTCAAC GT[CT]^[GA]AC
HindIII 570 AAGCTT A^AGCTT
MaeII 2362 ACGT A^CGT
MfeI 2339 CAATTG C^AATTG
MunI 2339 CAATTG C^AATTG
NaeI 144 GCCGGC GCC^GGC
NciI 126 CCGGG CC^[GC]GG
NgoMIV 144 GCCGGC G^CCGGC
NspV 574 TTCGAA TT^CGAA
RcaI 1111 TCATGA T^CATGA
ScaI 440 AGTACT AGT^ACT
SfaNI 267 GCATC GCATC(5/9)
SfuI 574 TTCGAA TT^CGAA
SpeI 2645 ACTAGT A^CTAGT
StuI 1282 AGGCCT AGG^CCT
StyI 463 CCAAGG C^C[AT][AT]GG
XbaI 1157 TCTAGA T^CTAGA
XmnI 438 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BanII 229 GGGCTC G[GA]GC[CT]^C
236 GAGCCC
BlpI 23 GCTAAGC GC^T[ACGT]AGC
1785 GCTCAGC
Bpu1102I 23 GCTAAGC GC^T[ACGT]AGC
1785 GCTCAGC
BspLU11I 623 ACATGT A^CATGT
830 ACATGT
BsrDI 660 GCAATG GCAATG(2/0)
1732 GCAATG
BssHII 93 GCGCGC G^CGCGC
108 GCGCGC
CelII 23 GCTAAGC GC^T[ACGT]AGC
1785 GCTCAGC
EaeI 14 TGGCCG [CT]^GGCC[GA]
146 CGGCCA
NdeI 283 CATATG CA^TATG
540 CATATG
PflMI 1686 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2346 CCAGTGAATGG
SmlI 552 CTTGAG C^T[CT][GA]AG
1175 CTCAAG
SspI 1954 AATATT AAT^ATT
2700 AATATT
TaqI 340 TCGA T^CGA
575 TCGA
TthHB8I 340 TCGA T^CGA
575 TCGA
Van91I 1686 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2346 CCAGTGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AvaII
BbsI BbvCI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI SspBI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII